1887

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Volume 56, Issue 10

Genotypic analyses of uropathogenic based on single nucleotide polymorphisms (SNPs) Free

Abstract

The application of genotyping techniques for subtyping uropathogenic has contributed to better understanding of the epidemiology of community-acquired urinary tract infection (UTI). However, the current techniques are hampered by limited reproducibility, poor discriminatory power, labour-intensive performance or high cost. A screening test that is sequence-based would provide an inexpensive, reproducible way to subtype isolates. Such a test, if also discriminatory, would be highly useful for epidemiological studies. The discriminatory ability of 12 putative virulence genes (, , , , , , , , , , , ) was evaluated based on single nucleotide polymorphisms (SNPs) in nine uropathogenic isolates, all previously found to belong to a single multilocus sequence type (MLST) complex (ST69). An additional 25 epidemiologically well-characterized isolates belonging to 12 distinct MLST clonal complexes were analysed for SNP. None of the 12 genes except were able to further discriminate the nine ST69-complex strains. Isolates belonging to the 12 non-ST69 MLST groups were separated into 10 SNP subgroups. While SNP analysis may not be an appropriate phylogenetic method, it offers discriminatory power similar to that of MLST and could be used as a simple, inexpensive screening test for epidemiological studies of uropathogenic .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ERIC-PCR, Enterobacterial Repetitive Intergenic Consensus-PCR , ExPEC, extraintestinal pathogenic Escherichia coli , MLST, multilocus sequence type , SNP, single nucleotide polymorphism and UTI, urinary tract infection
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2007-10-01
2024-04-18
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Genotypic analyses of uropathogenic Escherichia coli based on fimH single nucleotide polymorphisms (SNPs)
J. Med. Microbiol. 56, 1363 (2007); https://doi.org/10.1099/jmm.0.47262-0
/content/journal/jmm/10.1099/jmm.0.47262-0
/content/journal/jmm/10.1099/jmm.0.47262-0
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