1887

Abstract

The aim of this study was to biotype and characterize phospholipase, proteinase and haemolytic activities of oral isolates from 210 Chinese patients with type 2 diabetes mellitus (DM) and 210 age- and sex-matched healthy controls. Seventy-six and 50 isolates were obtained from type 2 DM patients and controls, respectively, using the oral rinse technique. The isolates were characterized with a biotyping system based on enzyme profiles, carbohydrate assimilation patterns and boric acid resistance of the yeasts, and the isolates were further tested for phospholipase, proteinase and haemolytic activities. The major biotypes of isolates from the type 2 DM and control groups were A1R (42.1 %) and J1R (36.0 %), respectively. Significantly higher proteinase and haemolytic activities were found in the isolates from the type 2 DM group (<0.05). Proteinase activity was higher in isolates from patients with ≥10 years of DM history than those with <10 years (<0.05). Haemolytic activity was significantly higher in isolates from female DM patients than in those from male counterparts (<0.05). These data provide evidence of increased extracellular enzyme activity in isolates taken from DM patients.

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2007-10-01
2024-03-29
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References

  1. Alberti K. G., Zimmet P. Z. 1998; Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med 15:539–553 [CrossRef]
    [Google Scholar]
  2. Belazi M., Velegraki A., Fleva A., Gidarakou I., Papanaum L., Baka D., Daniilidou N., Karamitsos D. 2005; Candidal overgrowth in diabetic patients: potential predisposing factors. Mycoses 48:192–196 [CrossRef]
    [Google Scholar]
  3. Collin H. L., Sorsa T., Meurman J. H., Niskanen L., Salo T., Ronka H., Konttinen Y. T., Koivisto A. M., Uusitupa M. 2000; Salivary matrix metalloproteinase (MMP-8) levels and gelatinase (MMP-9) activities in patients with type 2 diabetes mellitus. J Periodontal Res 35:259–265 [CrossRef]
    [Google Scholar]
  4. Darwazeh A. M., MacFarlane T. W., McCuish A., Lamey P. J. 1991; Mixed salivary glucose levels and candidal carriage in patients with diabetes mellitus. J Oral Pathol Med 20:280–283 [CrossRef]
    [Google Scholar]
  5. Kadir T., Pisiriciler R., Akyüz S., Yarat A., Emekli N., Ipbüker A. 2002; Mycological and cytological examination of oral candidal carriage in diabetic patients and non-diabetic control subjects: thorough analysis of local aetiologic and systemic factors. J Oral Rehabil 29:452–457 [CrossRef]
    [Google Scholar]
  6. Kantarcioğlu A. S., Yücel A. 2002; Phospholipase and protease activities in clinical Candida isolates with reference to the sources of strains. Mycoses 45:160–165 [CrossRef]
    [Google Scholar]
  7. Koga-Ito C. Y., Lyon J. P., Vidotto V., Resende M. A. 2006; Virulence factors and antifungal susceptibility of Candida albicans isolates from oral candidiasis patients and control individuals. Mycopathologia 161:219–223 [CrossRef]
    [Google Scholar]
  8. Leung W. K., Dassanayake R. S., Yau J. Y., Jin L. J., Yam W. C., Samaranayake L. P. 2000; Oral colonization, phenotypic and genotypic profiles of Candida species in irradiated, dentate, xerostomic nasopharyngeal carcinoma survivors. J Clin Microbiol 38:2219–2226
    [Google Scholar]
  9. Luo G., Samaranayake L. P., Yau J. Y. Y. 2001; Candida species exhibit differential in vitro hemolytic activities. J Clin Microbiol 39:2971–2974 [CrossRef]
    [Google Scholar]
  10. Luo G., Samaranayake L. P., Cheung B. P. K., Tang G. 2004; Reverse transcriptase polymerase chain reaction (RT-PCR) detection of HLP gene expression in Candida glabrata and its possible role in in vitro haemolysin production. APMIS 112:283–290 [CrossRef]
    [Google Scholar]
  11. Manfredi M., McCullough M. J., Al-Karaawi Z. M., Vescovi P., Porter S. R. 2006; In vitro evaluation of virulence attributes of Candida spp. isolated from patients affected by diabetes mellitus. Oral Microbiol Immunol 21:183–189 [CrossRef]
    [Google Scholar]
  12. Manns J. M., Mosser D. M., Buckley H. R. 1994; Production of a hemolytic factor by Candida albicans . Infect Immun 62:5154–5156
    [Google Scholar]
  13. Naglik J. R., Rodgers C. A., Shirlaw P. J., Dobbie J. L., Fernandes-Naglik L. L., Greenspan D., Agabian N., Challacombe S. J. 2003; Differential expression of Candida albicans secreted aspartyl proteinase and phospholipase B genes in humans correlates with active oral and vaginal infections. J Infect Dis 188:469–479 [CrossRef]
    [Google Scholar]
  14. Odds F. C. 1998 Candida and candidosis: a review and bibliography London: Bailliere Tindall;
    [Google Scholar]
  15. Price M. F., Wilkinson I. D., Gentry L. O. 1982; Plate method for detection of phospholipase activity in Candida albicans . Sabouraudia 20:7–14 [CrossRef]
    [Google Scholar]
  16. Rayfield E. J., Ault M. J., Keusch G. T., Brothers M. J., Nechemias C., Smith H. 1982; Infection and diabetes: the case for glucose control. Am J Med 72:439–450 [CrossRef]
    [Google Scholar]
  17. Samaranayake L. P. 1990; Host factors and oral candidosis. In Oral Candidosis pp 66–103 Edited by Samaranayake L. P., MacFarlane T. W. London: Wright;
    [Google Scholar]
  18. Samaranayake L. P., Raeside J. M., MacFarlane T. W. 1984; Factors affecting the phospholipase activity of Candida species in vitro. Sabouraudia 22:201–207 [CrossRef]
    [Google Scholar]
  19. Samaranayake Y. H., Samaranayake L. P., Yau J. Y. Y., Dassanayake R. S., Li T. K. L., Anil S. 2003; Phenotypic diversity of oral C. albicans isolated on single and sequential visits in an HIV-infected Chinese cohort. APMIS 111:329–337 [CrossRef]
    [Google Scholar]
  20. Samaranayake Y. H., Dassanayake R. S., Cheung B. P. K., Jayatilake J. A., Yeung K. W. S., Yau J. Y. Y., Samaranayake L. P. 2006; Differential phospholipase gene expression by Candida albicans in artificial media and cultured human oral epithelium. APMIS 114:857–866 [CrossRef]
    [Google Scholar]
  21. Schaller M., Borelli C., Korting H. C., Hube B. 2005; Hydrolytic enzymes as virulence factors of Candida albicans . Mycoses 48:365–377 [CrossRef]
    [Google Scholar]
  22. Shimizu M. T., Almeida N. Q., Fantinato V., Unterkircher C. S. 1996; Studies on hyaluronidase, chondroitin sulphatase, proteinase and phospholipase secreted by Candida species. Mycoses 39:161–167 [CrossRef]
    [Google Scholar]
  23. Soysa N. S., Samaranayake L. P., Ellepola A. N. B. 2006; Diabetes mellitus as a contributory factor in oral candidosis. Diabet Med 23:455–459 [CrossRef]
    [Google Scholar]
  24. Staib F. 1965; Serum-proteins as nitrogen source for yeastlike fungi. Sabouraudia 4:187–193
    [Google Scholar]
  25. Stevens D. A., Odds F. C., Scherer S. 1990; Application of DNA typing methods to Candida albicans epidemiology and correlations with phenotype. Rev Infect Dis 12:258–266 [CrossRef]
    [Google Scholar]
  26. Tsang C. S. P., Samaranayake L. P. 2000; Oral yeasts and coliforms in HIV-infected individuals in Hong Kong. Mycoses 43:303–308 [CrossRef]
    [Google Scholar]
  27. Tsang P. C., Samaranayake L. P., Philipsen H. P., McCulloug M., Reichart P. A., Schmidt-Westhausen A., Scully C., Porter S. R. 1995; Biotypes of oral Candida albicans isolates in human immunodeficiency virus-infected patients from diverse geographic locations. J Oral Pathol Med 24:32–36 [CrossRef]
    [Google Scholar]
  28. Weinberg E. D. 1978; Iron and infection. Microbiol Rev 42:45–66
    [Google Scholar]
  29. Williamson M. I., Samaranayake L. P., MacFarlane T. W. 1987; A new simple method for biotyping Candida albicans . Microbios 51:159–167
    [Google Scholar]
  30. Wu T., Samaranayake L. P., Cao B. Y., Wang J. 1996; In-vitro proteinase production by oral Candida albicans isolates from individuals with and without HIV infection and its attenuation by antimycotic agents. J Med Microbiol 44:311–316 [CrossRef]
    [Google Scholar]
  31. Xu Y. Y., Samaranayake L. P. 1995; Oral Candida albicans biotypes in Chinese patients with and without oral candidosis. Arch Oral Biol 40:577–579 [CrossRef]
    [Google Scholar]
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