1887

Abstract

A serial multiplex PCR approach was reformulated for pneumococcal serotyping to test 153 clinical isolates from children in Mozambique. This approach identified serotypes in 139 (90.8 %) of 153 isolates; 126 (82.4 %) were identified within two reactions. This approach in developing countries would require minimal training and could provide useful serotype information without requiring transport of specimens.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47346-0
2007-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/56/9/1181.html?itemId=/content/journal/jmm/10.1099/jmm.0.47346-0&mimeType=html&fmt=ahah

References

  1. Berkley J. A., Lowe I., Mwangi B. S., Williams T., Bauni E., Mwarumba S., Ngetsa C., Slack M. P., Njenga S. other authors 2005; Bacteremia among children admitted to a rural hospital in Kenya. N Engl J Med 352:39–47 [CrossRef]
    [Google Scholar]
  2. Dias C. A., Teixeira L. M., Carvalho Mda. G., Beall B. 2007; Sequential multiplex PCR for determining capsular serotypes of pneumococci recovered from Brazilian children. J Med Microbiol 56:1185–1188 [CrossRef]
    [Google Scholar]
  3. Hausdorff W. P., Bryant J., Paradiso P. R., Siber G. R. 2000; Which pneumococcal serogroups cause the most invasive disease: implications for conjugate vaccine formulation and use, part I. Clin Infect Dis 30:100–121 [CrossRef]
    [Google Scholar]
  4. Pai R., Gertz R. E., Beall B. 2006; Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates. J Clin Microbiol 44:124–131 [CrossRef]
    [Google Scholar]
  5. Roca A., Sigauque B., Quinto L., Mandomando I., Valles X., Espasa M., Abacassamo F., Sacarlal J., Macete E. other authors 2006; Invasive pneumococcal disease in children <5 years of age in rural Mozambique. Trop Med Int Health 11:1422–1431 [CrossRef]
    [Google Scholar]
  6. Valles X., Flannery B., Roca A., Mandomando I., Sigauque B., Sanz S., Schuchat A., Levine M., Soriano-Gabarro M., Alonso P. 2006; Serotype distribution and antibiotic susceptibility of invasive and nasopharyngeal isolates of Streptococcus pneumoniae among children in rural Mozambique. Trop Med Int Health 11:358–366 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47346-0
Loading
/content/journal/jmm/10.1099/jmm.0.47346-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error