@article{mbs:/content/journal/jmm/10.1099/jmm.0.47347-0, author = "Dias, Cícero A. and Teixeira, Lúcia Martins and Carvalho, Maria da Glória and Beall, Bernard", title = "Sequential multiplex PCR for determining capsular serotypes of pneumococci recovered from Brazilian children", journal= "Journal of Medical Microbiology", year = "2007", volume = "56", number = "9", pages = "1185-1188", doi = "https://doi.org/10.1099/jmm.0.47347-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.47347-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "CDC, Centers for Disease Control and Prevention", abstract = "Capsular serotype surveillance of clinical isolates of Streptococcus pneumoniae is essential for evaluation of the potential impact of introducing multivalent capsular serotype-based vaccines in Latin America. Here, a previously described sequential multiplex PCR method was revised for optimal targeting of prevalent serotypes in Latin America. The revised protocol successfully serotyped 139/147 pneumococci (94.6 %) from Brazilian children, demonstrating a labour-efficient, accurate method requiring only conventional PCR capability.", }