@article{mbs:/content/journal/jmm/10.1099/jmm.0.47363-0, author = "Yamazaki-Matsune, Wataru and Taguchi, Masumi and Seto, Kazuko and Kawahara, Ryuji and Kawatsu, Kentaro and Kumeda, Yuko and Kitazato, Miyoshi and Nukina, Masafumi and Misawa, Naoaki and Tsukamoto, Teizo", title = "Development of a multiplex PCR assay for identification of Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis", journal= "Journal of Medical Microbiology", year = "2007", volume = "56", number = "11", pages = "1467-1473", doi = "https://doi.org/10.1099/jmm.0.47363-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.47363-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "A multiplex PCR assay has been developed for the identification of the six common Campylobacter taxa associated with human gastroenteritis and/or septicaemia, namely Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis. The assay was developed using a combination of newly designed and published primers. It provided a specific PCR product for each of the five Campylobacter species and the one subspecies, and each of the PCR products was sufficiently distinguished by a difference in size by agarose gel electrophoresis. On evaluation of efficacy with 142 Campylobacter strains, the assay correctly identified all strains as 1 of the 6 Campylobacter taxa. This multiplex PCR assay is a rapid, simple and practical tool for identification of the six Campylobacter taxa commonly associated with gastroenteritis and/or septicaemia in humans, and offers an effective alternative to conventional biochemical-based assays.", }