RT Journal Article SR Electronic(1) A1 Yamazaki-Matsune, Wataru A1 Taguchi, Masumi A1 Seto, Kazuko A1 Kawahara, Ryuji A1 Kawatsu, Kentaro A1 Kumeda, Yuko A1 Kitazato, Miyoshi A1 Nukina, Masafumi A1 Misawa, Naoaki A1 Tsukamoto, TeizoYR 2007 T1 Development of a multiplex PCR assay for identification of Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis JF Journal of Medical Microbiology, VO 56 IS 11 SP 1467 OP 1473 DO https://doi.org/10.1099/jmm.0.47363-0 PB Microbiology Society, SN 1473-5644, AB A multiplex PCR assay has been developed for the identification of the six common Campylobacter taxa associated with human gastroenteritis and/or septicaemia, namely Campylobacter coli, Campylobacter fetus, Campylobacter hyointestinalis subsp. hyointestinalis, Campylobacter jejuni, Campylobacter lari and Campylobacter upsaliensis. The assay was developed using a combination of newly designed and published primers. It provided a specific PCR product for each of the five Campylobacter species and the one subspecies, and each of the PCR products was sufficiently distinguished by a difference in size by agarose gel electrophoresis. On evaluation of efficacy with 142 Campylobacter strains, the assay correctly identified all strains as 1 of the 6 Campylobacter taxa. This multiplex PCR assay is a rapid, simple and practical tool for identification of the six Campylobacter taxa commonly associated with gastroenteritis and/or septicaemia in humans, and offers an effective alternative to conventional biochemical-based assays., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.47363-0