1887

Abstract

causes dental caries and infective endocarditis. The aim of this study was to determine genomic diversity among serotype laboratory and clinical strains and to characterize the genetic events involved. A genome-based approach using PFGE coupled with Southern hybridization was employed to examine a total of 58 serotype oral and blood isolates and seven laboratory strains and to compare them with UA159. No significant differences were found in the phenotypic characteristics of the strains tested, except that some of the strains exhibited smooth rather than rough colony morphology. In contrast, PFGE profiles of clinical isolates, from either diseased or healthy subjects, exhibited diverse patterns, suggesting that recombination or point mutations occurred frequently . Diverse PFGE patterns, with various lengths of insertions and deletions, could be detected even within a localized chromosomal region between rRNA operons. Comparative analysis using Southern hybridization with specific markers revealed that a large chromosomal inversion had also occurred between operons in 25 strains.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47428-0
2008-02-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jmm/57/2/198.html?itemId=/content/journal/jmm/10.1099/jmm.0.47428-0&mimeType=html&fmt=ahah

References

  1. Ajdic D., McShan W. M., McLaughlin R. E., Savic G., Chang J., Carson M. B., Primeaux C., Tian R., Kenton S. other authors 2002; Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. Proc Natl Acad Sci U S A 99:14434–14439 [CrossRef]
    [Google Scholar]
  2. Aspiras M. B., Kazmerzak K. M., Kolenbrander P. E., McNab R., Hardegen N., Jenkinson H. F. 2000; Expression of green fluorescent protein in Streptococcus gordonii DL1 and its use as a species-specific marker in coadhesion with Streptococcus oralis 34 in saliva-conditioned biofilms in vitro . Appl Environ Microbiol 66:4074–4083 [CrossRef]
    [Google Scholar]
  3. Banas J. A., Vickerman M. M. 2003; Glucan-binding proteins of the oral streptococci. Crit Rev Oral Biol Med 14:89–99 [CrossRef]
    [Google Scholar]
  4. Beighton D., Russell R. R. B., Whiley R. A. 1991; A simple biochemical scheme for the differentiation of Streptococcus mutans and Streptococcus sobrinus . Caries Res 25:174–178 [CrossRef]
    [Google Scholar]
  5. Cappiello M. G., Hantman M. J., Zuccon F. M., Peruzzi F., Amjad M., Piggot P. J., Daneo-Moore L. 1999; Physical and genetic map of Streptococcus mutans GS-5 and localization of five rRNA operons. Oral Microbiol Immunol 14:225–232 [CrossRef]
    [Google Scholar]
  6. Chia J. S., Hsu T. Y., Teng L. J., Chen J. Y., Hahn L. J., Yang C. S. 1991; Glucosyltransferase gene polymorphism among Streptococcus mutans strains. Infect Immun 59:1656–1660
    [Google Scholar]
  7. Crowley P. J., Brady L. J., Michalek S. M., Bleiweis A. S. 1999; Virulence of a spaP mutant of Streptococcus mutans in a gnotobiotic rat model. Infect Immun 67:1201–1206
    [Google Scholar]
  8. Davey M. E., O'Toole G. A. 2000; Microbial biofilms: from ecology to molecular genetics. Microbiol Mol Biol Rev 64:847–867 [CrossRef]
    [Google Scholar]
  9. Gronroos L., Alaluusua S. 2000; Site-specific oral colonization of mutans streptococci detected by arbitrarily primed PCR fingerprinting. Caries Res 34:474–480 [CrossRef]
    [Google Scholar]
  10. Hamada S., Tai S., Slade H. D. 1976; Selective adsorption of heterophile polyglycerophosphate antigen from antigen extracts of Streptococcus mutans and other gram-positive bacteria. Infect Immun 14:903–910
    [Google Scholar]
  11. Helm R. A., Lee A. G., Christman H. D., Maloy S. 2003; Genomic rearrangements at rrn operons in Salmonella . Genetics 165:951–959
    [Google Scholar]
  12. Hillman J. D., Johnson K. P., Yaphe B. I. 1984; Isolation of a Streptococcus mutans strain producing a novel bacteriocin. Infect Immun 44:141–144
    [Google Scholar]
  13. Holden M. T., Scott A., Cherevach I., Chillingworth T., Churcher C., Cronin A., Dowd L., Feltwell T., Hamlin N. other authors 2007; Complete genome of acute rheumatic fever-associated serotype M5 Streptococcus pyogenes strain Manfredo. J Bacteriol 189:1473–1477 [CrossRef]
    [Google Scholar]
  14. Koga T., Asakawa H., Okahashi N., Takahashi I. 1989; Effect of subculturing on expression of a cell-surface protein antigen by Streptococcus mutans . J Gen Microbiol 135:3199–3207
    [Google Scholar]
  15. Kuramitsu H. K. 1975; Characterization of extracellular glucosyltransferase activity of Steptococcus mutans . Infect Immun 12:738–749
    [Google Scholar]
  16. Loesche W. J. 1986; Role of Streptococcus mutans in human dental decay. Microbiol Rev 50:353–380
    [Google Scholar]
  17. Macrina F. L., Jones K. R., Laloi P. 1996; Characterization of IS 199 from Streptococcus mutans V403. Plasmid 36:9–18 [CrossRef]
    [Google Scholar]
  18. Martin J. M., Wald E. R., Green M. 1998; Field inversion gel electrophoresis as a typing system for group A streptococcus. J Infect Dis 177:504–507 [CrossRef]
    [Google Scholar]
  19. Mylonakis E., Calderwood S. B. 2001; Infective endocarditis in adults. N Engl J Med 345:1318–1330 [CrossRef]
    [Google Scholar]
  20. Nakagawa I., Kurokawa K., Yamashita A., Nakata M., Tomiyasu Y., Okahashi N., Kawabata S., Yamazaki K., Shiba T. other authors 2003; Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution. Genome Res 13:1042–1055 [CrossRef]
    [Google Scholar]
  21. Nomura R., Nakano K., Ooshima T. 2005; Molecular analysis of the genes involved in the biosynthesis of serotype specific polysaccharide in the novel serotype k strains of Streptococcus mutans . Oral Microbiol Immunol 20:303–309 [CrossRef]
    [Google Scholar]
  22. O'Toole G. A., Kolter R. 1998; Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signaling pathways: a genetic analysis. Mol Microbiol 28:449–461 [CrossRef]
    [Google Scholar]
  23. Okahashi N., Asakawa H., Koga T., Masuda N., Hamada S. 1984; Clinical isolates of Streptococcus mutans serotype c with altered colony morphology due to fructan synthesis. Infect Immun 44:617–622
    [Google Scholar]
  24. Okahashi N., Sasakawa C., Okada N., Yamada M., Yoshikawa M., Tokuda M., Takahashi I., Koga T. 1990; Construction of Not I restriction map of the Streptococcus mutans genome. J Gen Microbiol 136:2217–2223 [CrossRef]
    [Google Scholar]
  25. Pebay M., Colmin C., Guedon G., De Gasperi C., Decaris B., Simonet J. M. 1992; Detection of intraspecific DNA polymorphism in Streptococcus salivarius subsp. thermophilus by a homologous rDNA probe. Res Microbiol 143:37–46 [CrossRef]
    [Google Scholar]
  26. Perry D., Kuramitsu H. K. 1981; Genetic transformation of Streptococcus mutans . Infect Immun 32:1295–1297
    [Google Scholar]
  27. Roussel Y., Bourgoin F., Guedon G., Pebay M., Decaris B. 1997; Analysis of the genetic polymorphism between three Streptococcus thermophilus strains by comparing their physical and genetic organization. Microbiology 143:1335–1343 [CrossRef]
    [Google Scholar]
  28. Tao L., MacAlister T. J., Tanzer J. M. 1993; Transformation efficiency of EMS-induced mutants of Streptococcus mutans of altered cell shape. J Dent Res 72:1032–1039 [CrossRef]
    [Google Scholar]
  29. Tillier E. R., Collins R. A. 2000; Genome rearrangement by replication-directed translocation. Nat Genet 26:195–197 [CrossRef]
    [Google Scholar]
  30. Waterhouse J. C., Russell R. R. B. 2006; Dispensable genes and foreign DNA in Streptococcus mutans . Microbiology 152:1777–1788 [CrossRef]
    [Google Scholar]
  31. Waterhouse J. C., Swan D. C., Russell R. R. B. 2007; Comparative genome hybridization of Streptococcus mutans strains. Oral Microbiol Immunol 22:103–110 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47428-0
Loading
/content/journal/jmm/10.1099/jmm.0.47428-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error