1887

Journal of Medical Microbiology logo

Volume 57, Issue 7

Emergence of CTX-M-15 type extended-spectrum -lactamase-producing spp. in Kuwait and the United Arab Emirates Free

Abstract

Cephalosporins are major antimicrobials used to treat serious infections. However, their effectiveness is being compromised by the emergence of extended-spectrum -lactamases (ESBLs). The genetic determinants encoding ESBL in spp. isolated from patients in Kuwait and United Arab Emirates (UAE) were studied over a 2 year period. Out of a total of 407 isolates, 116 isolates possessed the resistance phenotypes consistent with possible ESBL production. Of these, 69 (59.5 %) were ESBL positive. PCR and sequencing were used to determine the genetic determinant(s) responsible for ESBL phenotypes. A total of 14 (12.1 %) and 29 (24.6 %) isolates were CTX-M-15 ESBL producers and TEM producers, respectively. Ten CTX-M-15 producers carried the insertion sequence ISI gene. PFGE analysis revealed identical profiles in 4 of the 13 Kuwaiti strains. This study reports the presence of the gene in spp. and serotype Typhi from Kuwait and UAE for what is believed to be the first time. This is of great concern as the gene is also found in association with the ISI gene, which may easily facilitate its spread. These isolates originated mostly from non-Kuwaiti Arabs rather than from people of Asian origin.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Amp, ampicillin , Caz, ceftazidime , Cro, ceftriaxone , Ctx, cefotaxime , Cxm, cefuroxime , ESBL, extended-spectrum β-lactamase and UAE, United Arab Emirates
SGM
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47509-0
2008-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/57/7/881.html?itemId=/content/journal/jmm/10.1099/jmm.0.47509-0&mimeType=html&fmt=ahah

References

  1. Arlet G., Barrett T. J., Butaye P., Cloeckaert A., Mulvey M. R., White D. G. 2006; Salmonella resistance to extended-spectrum cephalosporins: prevalence and epidemiology. Microbes Infect 8:1945–1954 [CrossRef]
     [Google Scholar]
  2. Batchelor M., Hopkins K., Threlfall E. J., Clifton-Hadley F. A., Stallwood A. D., Davies R. H., Liebana E. 2005; bla CTX-M genes in clinical Salmonella isolates recovered from humans in England and Wales from 1992 to 2003. Antimicrob Agents Chemother 49:1319–1322 [CrossRef]
     [Google Scholar]
  3. Bauernfeind A., Stemplinger I., Jungwirth R., Ernst S., Casellas J. M. 1996; Sequences of β -lactamase genes encoding CTX-M-1 (MEN-1) and CTX-M-2 and relationship of their amino acid sequences with those of other β -lactamases. Antimicrob Agents Chemother 40:509–513
     [Google Scholar]
  4. Bonnet R. 2004; Growing group of extended-spectrum β -lactamases: the CTX-M enzymes. Antimicrob Agents Chemother 48:1–14 [CrossRef]
     [Google Scholar]
  5. Bradford P. A. 2001; Extended-spectrum β -lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev 14:933–951 [CrossRef]
     [Google Scholar]
  6. Bradford P. A., Yang Y., Sahm D., Grope I., Gardovska D., Storch G. 1998; CTX-M-5, a novel cefotaxime-hydrolyzing β -lactamase from an outbreak of Salmonella typhimurium in Latvia. Antimicrob Agents Chemother 42:1980–1984
     [Google Scholar]
  7. Cao V., Lambert T., Courvalin P. 2002; ColE1-like plasmid pIP843 of Klebsiella pneumoniae encoding extended-spectrum β -lactamase CTX-M-17. Antimicrob Agents Chemother 46:1212–1217 [CrossRef]
     [Google Scholar]
  8. CDC 2002 Standardized Molecular Subtyping of Foodborne Bacterial Pathogens by Pulsed-Field Gel Electrophoresis Atlanta, GA: Centers for Disease Control and Prevention;
     [Google Scholar]
  9. CLSI 2005 Performance Standard for Antimicrobial Susceptibility Testing , M100-S15 Wayne, PA: Clinical Laboratory Standards Institute;
     [Google Scholar]
  10. Dunne E. F., Fey P. D., Kludt P., Reporter R., Mostashari F., Shillam P., Wicklund J., Miller C., Holland B. other authors 2000; Emergence of domestically acquired ceftriaxone-resistant Salmonella infections associated with AmpC β -lactamases. JAMA 284:3151–3156 [CrossRef]
     [Google Scholar]
  11. Edelstein M., Pimkin M., Dmitrachenko T., Semenov V., Kozlova N., Gladin D., Baraniak A., Stratchounski L. 2004; Multiple outbreaks of salmonellosis in Russia and Belarus caused by a single clone of Salmonella enterica serovar Typhimurium producing an extended-spectrum β -lactamase. Antimicrob Agents Chemother 48:2808–2815 [CrossRef]
     [Google Scholar]
  12. Hammami A., Arlet G., Ben Redjeb S., Grimont F., Ben Hassen A., Rekik A., Philippon A. 1991; Nosocomial outbreak of acute gastroenteritis in a neonatal intensive care unit in Tunisia caused by multiply drug resistant Salmonella wien producing SHV-2 β -lactamase. Eur J Clin Microbiol Infect Dis 10:641–646 [CrossRef]
     [Google Scholar]
  13. Jamal W. Y., Pal T., Rotimi V. O., Chugh T. D. 1998; Serogroups and antimicrobial susceptibility of clinical isolates of Salmonella species from a teaching hospital in Kuwait. J Diarrhoeal Dis Res 16:180–186
     [Google Scholar]
  14. Jin Y., Ling J. M. 2006; CTX-M-producing Salmonella spp. in Hong Kong: an emerging problem. J Med Microbiol 55:1245–1250 [CrossRef]
     [Google Scholar]
  15. Kim S., Hu J., Gautom R., Kim J., Lee B., Boyle D. S. 2007; CTX-M extended-spectrum β -lactamases, Washington State. Emerg Infect Dis 13:513–514 [CrossRef]
     [Google Scholar]
  16. Lartigue M.-F., Poirel L., Nordmann P. 2004; Diversity of genetic environment of bla CTX-M genes. FEMS Microbiol Lett 234:201–207 [CrossRef]
     [Google Scholar]
  17. Li W. C., Huang F. Y., Liu C. P., Weng L. C., Wang N. Y., Chiu N. C., Chiang C. S. 2005; Ceftriaxone resistance of nontyphoidal Salmonella enterica isolates in Northern Taiwan attributable to production of CTX-M14 and CMY-2 β -lactamases. J Clin Microbiol 43:3237–3243 [CrossRef]
     [Google Scholar]
  18. Livermore D. M., Hawkey P. M. 2005; CTX-M: changing the face of ESBLs in the UK. J Antimicrob Chemother 56:451–454 [CrossRef]
     [Google Scholar]
  19. Miriagou V., Tzouvelekis L. S., Rossiter S., Tzelepi E., Angulo F. J., Whichard J. 2003; Imipenem resistance in a Salmonella strain due to plasmid-mediated class A carbapenemase KPC-2. Antimicrob Agents Chemother 47:1297–1300 [CrossRef]
     [Google Scholar]
  20. Miriagou V., Tassios P. T., Legakis N. J., Tzouvelekis L. S. 2004; Expanded-spectrum cephalosporin resistance in non-typhoid Salmonella . Int J Antimicrob Agents 23:547–555 [CrossRef]
     [Google Scholar]
  21. Morris D., Whelan M., Corbett-Feeney G., Cormican M., Hawkey P., Li X., Doran G. 2006; First report of extended-spectrum- β -lactamase-producing Salmonella enterica isolates in Ireland. Antimicrob Agents Chemother 50:1608–1609 [CrossRef]
     [Google Scholar]
  22. Moubareck C., Doucet-Populaire F., Hamez M., Daoud Z., Weill F. X. 2005; First extended-spectrum β -lactamase (CTX-M-15) producing Salmonella enterica serotype Typhimurium isolate identified in Lebanon. Antimicrob Agents Chemother 49:864–865 [CrossRef]
     [Google Scholar]
  23. Orman B. E., Pineiro S. A., Arduino S., Galas M., Melano R., Caffer M. I., Sordelli D. O., Centron D. 2002; Evolution of multiresistance in non-typhoid Salmonella serovars from 1984 to 1998 in Argentina. Antimicrob Agents Chemother 46:3963–3970 [CrossRef]
     [Google Scholar]
  24. Poirel L., Gniadkowski M., Nordmann P. 2002; Biochemical analysis of ceftazidime-hydrolysing extended-spectrum β -lactamase CTX-M-15 and of its structurally related β -lactamase CTX-M-3. J Antimicrob Chemother 50:1031–1034 [CrossRef]
     [Google Scholar]
  25. Poirel L. P., Rotimi V. O., Bernabeu S., Jamal W., Nordmann P. 2007; Explosive emergence of CTX-M-15 extended-spectrum β -lactamase in Enterobacteriaceae in Kuwait. In 17th European Congress of Clinical Microbiology and Infectious Diseases. Munich, Germany, abstract no. P1029 p 114
     [Google Scholar]
  26. Rodriguez M. M., Power P., Radice M., Vay C., Famiglietti A., Galleni M., Ayala A. J., Gutkind G. 2004; Chromosome-encoded CTX-M-3 from Kluyvera ascorbata : a possible origin of plasmid-borne CTX-M-1 derived cefotaximases. Antimicrob Agents Chemother 48:4895–4897 [CrossRef]
     [Google Scholar]
  27. Rossi A., Lopardo H., Woloj M., Picandet A. M., Marino M., Galds M., Radice M., Gutkind G. 1995; Non-typhoidal Salmonella spp. resistant to cefotaxime. J Antimicrob Chemother 36:697–702 [CrossRef]
     [Google Scholar]
  28. Su L. H., Wu T. L., Chia J. H., Chu C., Kuo A. J., Chiu C. H. 2005; Increasing ceftriaxone resistance in Salmonella isolates from a university hospital in Taiwan. J Antimicrob Chemother 55:846–852 [CrossRef]
     [Google Scholar]
  29. Tassios P. T., Gazouli M., Tzelepi E., Milch H., Kozlova N., Sidorenko S., Legakis N. J., Tzouvelekis L. S. 1999; Spread of a Salmonella typhimurium clone resistant to expanded-spectrum cephalosporins in three European countries. J Clin Microbiol 37:3774–3777
     [Google Scholar]
  30. Walther-Rasmussen J., Høiby N. 2004; Cefotaximases (CTX-M-ases), an expanding family of extended-spectrum β -lactamases. Can J Microbiol 50:137–165 [CrossRef]
     [Google Scholar]
  31. Winokur P. L., Canton R., Casellas J. M., Legakis N. J. 2001; Variations in the prevalence of strains expressing an extended-spectrum β -lactamase phenotype and characterization of isolates from Europe, the Americas and the Western Pacific region. Clin Infect Dis 32 (Suppl. 2):S94–S103 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47509-0
Loading
Emergence of CTX-M-15 type extended-spectrum β-lactamase-producing Salmonella spp. in Kuwait and the United Arab Emirates
J. Med. Microbiol. 57, 881 (2008); https://doi.org/10.1099/jmm.0.47509-0
/content/journal/jmm/10.1099/jmm.0.47509-0
/content/journal/jmm/10.1099/jmm.0.47509-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error