1887

Abstract

An imipenem-resistant isolate of ZJ163 (MIC 256 μg ml) isolated from a Chinese hospital was investigated. The ZJ163 isolate exhibited high-level resistance to carbapenems, penicillins, cephalosporins, cefoxitin, aztreonam, quinolones and aminoglycosides. Isoelectric focusing (IEF) demonstrated three -lactamases with pIs of 5.4 (TEM-1), 6.7 (KPC-2) and 7.9 (CTX-M-14). Two different transconjugants (types A and B) were obtained by conjugation studies. The type A transconjugant exhibited reduced susceptibility or resistance to penicillins, cephalosporins and aztreonam, but was susceptible to carbapenems, quinolones and aminoglycosides. The antimicrobial susceptibility patterns of the type B transconjugant were similar to that of type A, except for its significantly reduced carbapenem susceptibility (imipenem MIC 2 μg ml). IEF, specific PCRs and DNA sequence analysis indicated that the type A transconjugant produced CTX-M-14 -lactamase with a pI of 7.9, that the type B transconjugant produced KPC-2 -lactamase with a pI of 6.7 and that the -lactamase with a pI of 5.4 was TEM-1. PCR analysis and sequencing confirmed the presence of the gene in the chromosomal DNA from ZJ163, although no activity of AmpC -lactamase was detected by IEF. Urea/SDS-PAGE analysis of outer-membrane proteins revealed that the levels of the 41 and 38 kDa porins were decreased in ZJ163. It was concluded that production of KPC-2 combined with decreased expression of porins contributes to high-level resistance to carbapenems in ZJ163.

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2008-03-01
2024-03-29
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References

  1. Aoyama H., Fujimaki K., Sato K., Fujii T., Inoue M., Hirai K., Mitsuhashi S. 1988; Clinical isolate of Citrobacter freundii highly resistant to new quinolones. Antimicrob Agents Chemother 32:922–924 [CrossRef]
    [Google Scholar]
  2. Barlow M., Hall B. G. 2002; Origin and evolution of the AmpC β -lactamases of Citrobacter freundii . Antimicrob Agents Chemother 46:1190–1198 [CrossRef]
    [Google Scholar]
  3. Bratu S., Landman D., Alam M., Tolentino E., Quale J. 2005; Detection of KPC carbapenem-hydrolyzing enzymes in Enterobacter spp. from Brooklyn, New York. Antimicrob Agents Chemother 49:776–778 [CrossRef]
    [Google Scholar]
  4. Chen G. X., Zhang R., Zhou H. W. 2006; Heterogeneity of metallo- β -lactamases in clinical isolates of Chryseobacterium meningosepticum from Hangzhou, China. J Antimicrob Chemother 57:750–752 [CrossRef]
    [Google Scholar]
  5. Hernández-Allés S., Albertí S., Alvarez D., Doménech-Sánchez A., Martínez-Martínez L., Gil J., Tomás J. M., Benedí V. J. 1999; Porin expression in clinical isolates of Klebsiella pneumoniae . Microbiology 145:673–679 [CrossRef]
    [Google Scholar]
  6. Hossain A., Ferraro M. J., Pino R. M., Dew R. B. III, Moland E. S., Lockhart T. J., Thomson K. S., Goering R. V., Hanson N. D. 2004; Plasmid mediated carbapenem-hydrolyzing enzyme KPC-2 in an Enterobacter sp. Antimicrob Agents Chemother 48:4438–4440 [CrossRef]
    [Google Scholar]
  7. Koeleman J. G. M., Stoof J., Van Der Bijl M. W., Vandenbroucke-Grauls C. M. J. E., Savelkoul P. H. M. 2001; Identification of epidemic strains of Acinetobacter baumannii by integrase gene PCR. J Clin Microbiol 39:8–13 [CrossRef]
    [Google Scholar]
  8. Lévesque C., Piché L., Larose C., Roy P. H. 1995; PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob Agents Chemother 39:185–191 [CrossRef]
    [Google Scholar]
  9. Mathew A., Harris A. M., Marshall M. J., Ross G. W. 1975; The use of analytical isoelectric focusing for detection and identification of β -lactamase. J Gen Microbiol 88:169–178 [CrossRef]
    [Google Scholar]
  10. Miriagou V., Tzouvelekis L. S., Rossiter S., Tzelepi E., Angulo F. J., Whichard J. M. 2003; Imipenem resistance in a Salmonella clinical strain due to plasmid-mediated class A carbapenemase KPC-2. Antimicrob Agents Chemother 47:1297–1300 [CrossRef]
    [Google Scholar]
  11. Moland E. S., Hanson N. D., Herrera V. L., Black J. A., Lockhart T. J., Hossain A., Johnson J. A., Goering R. V., Thomson K. S. 2003; Plasmid-mediated, carbapenem-hydrolysing β -lactamase, KPC-2, in Klebsiella pneumoniae isolates. J Antimicrob Chemother 51:711–714 [CrossRef]
    [Google Scholar]
  12. Naas T., Nordmann P., Vedel G., Poyart C. 2005; Plasmid-mediated carbapenem-hydrolyzing β -lactamase KPC in a Klebsiella pneumoniae isolate from France. Antimicrob Agents Chemother 49:4423–4424 [CrossRef]
    [Google Scholar]
  13. NCCLS 2006 Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically . Approved standard M7-A7, 7th edn. Wayne, PA: National Committee for Clinical Laboratory Standards;
    [Google Scholar]
  14. Navon-Venezia S., Chmelnitsky I., Leavitt A., Schwaber M. J., Schwartz D., Carmeli Y. 2006; Plasmid-mediated imipenem-hydrolyzing enzyme KPC-2 among multiple carbapenem-resistant Escherichia coli clones in Israel. Antimicrob Agents Chemother 50:3098–3101 [CrossRef]
    [Google Scholar]
  15. Villegas M. V., Lolans K., Correa A., Suarez C. J., Lopez J. A., Vallejo M., Quinn J. P. The Colombian Nosocomial Resistance Study Group 2006; First detection of the plasmid-mediated class A carbapenemase KPC-2 in clinical isolates of Klebsiella pneumoniae from South America. Antimicrob Agents Chemother 50:2880–2882 [CrossRef]
    [Google Scholar]
  16. Villegas M. V., Lolans K., Correa A., Kattan J. N., Lopez J. A., Quinn J. P. The Colombian Nosocomial Resistance Study Group 2007; First identification of Pseudomonas aeruginosa isolates producing a KPC-type carbapenem-hydrolyzing β -lactamase. Antimicrob Agents Chemother 51:1553–1555 [CrossRef]
    [Google Scholar]
  17. Wei Z. Q., Du X. X., Yu Y. S., Shen P., Chen Y. G., Li L. J. 2007; Plasmid-mediated KPC-2 in a Klebsiella pneumoniae isolate from China. Antimicrob Agents Chemother 51:763–765 [CrossRef]
    [Google Scholar]
  18. Woodford N., Tierno P. M., Young K. Jr, Tysall L., Palepou M.-F. I., Ward E., Painter R. E., Suber D. F., Shungu D. other authors 2004; Outbreak of Klebsiella pneumoniae producing a new carbapenem-hydrolyzing class A β -lactamase, KPC-3, in a New York medical center. Antimicrob Agents Chemother 48:4793–4799 [CrossRef]
    [Google Scholar]
  19. Yigit H., Queenan A. M., Anderson G. J., Domenech-Sanchez A., Biddle J. W., Steward C. D., Alberti S., Bush K., Tenover F. C. 2001; Novel carbapenem-hydrolyzing β -lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae . Antimicrob Agents Chemother 45:1151–1161 [CrossRef]
    [Google Scholar]
  20. Yigit H., Queenan A. M., Rasheed J. K., Biddle J. W., Domenech-Sanchez A., Alberti S., Bush K., Tenover F. C. 2003; Carbapenem-resistant strain of Klebsiella oxytoca harboring carbapenem-hydrolyzing β -lactamase KPC-2. Antimicrob Agents Chemother 47:3881–3889 [CrossRef]
    [Google Scholar]
  21. Yu Y., Ji S., Chen Y., Zhou W., Wei Z., Li L., Ma Y. 2007; Resistance of strains producing extended-spectrum β -lactamases and genotype distribution in China. J Infect 54:53–57 [CrossRef]
    [Google Scholar]
  22. Zhang R., Zhou H. W., Cai J. C., Chen G. X. 2007; Plasmid-mediated carbapenem-hydrolysing β -lactamase KPC-2 in carbapenem-resistant Serratia marcescens isolates from Hangzhou, China. J Antimicrob Chemother 59:574–576 [CrossRef]
    [Google Scholar]
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