1887

Abstract

The aim of this study was to estimate differences in the prevalence of oral streptococcal species in the subgingival biofilm of patients with aggressive periodontitis and of healthy controls. Thirty-three patients with clinical and radiological proof of aggressive periodontitis and 20 healthy subjects were enrolled in this study. Clinical indices were recorded in a six-point measurement per tooth. Samples of the subgingival biofilm were taken with paper points from four teeth of each individual. Alpha- and non-haemolytic, small and catalase-negative colonies were biochemically identified using a rapid ID 32 STREP system and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A total of 118 strains of oral streptococci (11 species) were identified and was found significantly more often in healthy subjects (=0.001). Conversely, the absence of was associated with high values of clinical indices (=0.001–0.002). Aggressive periodontitis seems to be associated with a loss of colonization of . Whether or not offers protection against aggressive periodontitis needs to be determined. Otherwise, there were no significant differences in the distribution of oral streptococcal species in patients and healthy subjects.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47649-0
2008-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/57/4/495.html?itemId=/content/journal/jmm/10.1099/jmm.0.47649-0&mimeType=html&fmt=ahah

References

  1. Caufield P. W., Dasanayake A. P., Li Y., Pan Y., Hsu Y., Hardin M. 2000; Natural history of Streptococcus sanguinis in the oral cavity of infants: evidence for a discrete window of infectivity. Infect Immun 68:4018–4023 [CrossRef]
    [Google Scholar]
  2. Daep C. A., James D. M., Lamont R. J., Demuth D. R. 2006; Structural characterization of peptide-mediated inhibition of Porphyromonas gingivalis biofilm formation. Infect Immun 74:5756–5762 [CrossRef]
    [Google Scholar]
  3. Dowsett S. A., Kowolik M. J., Archila L. A., Eckert G. J., LeBlanc D. J. 2002; Subgingival microbiota of indigenous Indians of Central America. J Clin Periodontol 29:159–167 [CrossRef]
    [Google Scholar]
  4. Frandsen E. V., Pedrezzoli V., Killian M. 1991; Ecology of viridans streptococci in the oral cavity and pharynx. Oral Microbiol Immunol 6:129–133 [CrossRef]
    [Google Scholar]
  5. Friedrichs C., Rodloff A. C., Chhatwal S., Schellenberger W., Eschrich K. 2007; Rapid identification of viridans streptococci by mass spectrometric discrimination. J Clin Microbiol 45:2392–2397 [CrossRef]
    [Google Scholar]
  6. Haffajee A. D., Cugini M. A., Tanner A., Pollack R. P., Smith C., Kent R. L., Socransky S. S. 1998; Subgingival microbiota in healthy, well-maintained elder and periodontitis subjects. J Clin Periodontol 25:346–353 [CrossRef]
    [Google Scholar]
  7. Haffajee A. D., Teles R. P., Socransky S. S. 2006; Association of Eubacterium nodatum and Treponema denticola with human periodontitis lesions. Oral Microbiol Immunol 21:269–282 [CrossRef]
    [Google Scholar]
  8. Hammond B. F., Stevens R. H., Bonner P., Lillard S. E. 1984; Toxicity of Actinobacillus actinomycetemcomitans extracts for crevicular bacteria. J Dent Res 63:263 (abstract)
    [Google Scholar]
  9. Hillman J. D., Socransky S. S. 1982; Bacteria interference in the oral ecology of Actinobacillus actinomycetemcomitans and its relationship to human periodontosis. Arch Oral Biol 27:75–77 [CrossRef]
    [Google Scholar]
  10. Hillman J. D., Socransky S. S., Shivers M. 1985; The relationships between streptococcal species and periodontopathic bacteria in human dental plaque. Arch Oral Biol 30:791–795 [CrossRef]
    [Google Scholar]
  11. Hintao J., Teanpaisan R., Chonqsuvivatwong V., Ratarasan C., Dahlen G. 2007; The microbiological profiles of saliva, supragingival and subgingival plaque and dental caries in adults with and without type 2 diabetes mellitus. Oral Microbiol Immunol 22:175–181 [CrossRef]
    [Google Scholar]
  12. Kreth J., Merritt J., Shi W., Qi F. 2005; Competition and coexistence between Streptococcus mutans and Streptococcus sanguinis in the dental biofilm. J Bacteriol 187:7193–7203 [CrossRef]
    [Google Scholar]
  13. Lamont R. J., El-Sabaeny A., Park Y., Cook G. S., Costerton J. W., Demuth D. R. 2002; Role of the Streptococcus gordonii SspB protein in the development of Porphyromonas gingivalis biofilms on streptococcal substrates. Microbiology 148:1627–1636
    [Google Scholar]
  14. Maestre J. R., Bascones A., Sánchez P., Matesanz P., Aguilar L., Giménez M. J., Pérez-Balcabao I., Granizo J. J., Prieto J. 2007; Odontogenic bacteria in periodontal disease and resistance patterns to common antibiotics used as treatment and prophylaxis in odontology in Spain. Rev Esp Quimioter 20:61–67
    [Google Scholar]
  15. Marsh P. D. 1991; Sugar, fluoride, pH and microbial homeostasis in dental plaque. Proc Finn Dent Soc 87:515–525
    [Google Scholar]
  16. Marsh P. D. 2004; Dental plaque as a microbial biofilm. Caries Res 38:204–211 [CrossRef]
    [Google Scholar]
  17. Marsh P. D., Bradshaw D. J. 1999; Microbial community aspects of dental plaque. In Dental Plaque Revisited: Oral Biofilms in Health and Disease pp 237–253 Edited by Newman H. N., Wilson M. Cardiff: BioLine Publications;
    [Google Scholar]
  18. Quirynen M., DeSoete M., Dierickx K., van Steenberghe D. 2001; The intra-oral translocation of periodontal pathogens jeopardises the outcome of periodontal therapy. A review of the literature. J Clin Periodontol 28:499–507 [CrossRef]
    [Google Scholar]
  19. Rupf S., Breitung K., Schellenberger W., Merte K., Kneist S., Eschrich K. 2005; Differentiation of mutans streptococci by intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Oral Microbiol Immunol 20:267–273 [CrossRef]
    [Google Scholar]
  20. Smole S. C., King L. A., Leopold P. E., Arbeit R. D. 2002; Sample preparation of Gram positive bacteria for identification by matrix assisted laser desorption/ionization time-of-flight. J Microbiol Methods 48:107–115 [CrossRef]
    [Google Scholar]
  21. Socransky S. S., Haffajee A. D., Cugini M. A. 1998; Microbial complexes in subgingival plaque. J Clin Periodontol 25:134–144 [CrossRef]
    [Google Scholar]
  22. Teughels W., Haake K. S., Sliepen I., Pauwels M., van Eldere J., Cassiman J. J., Quirynen M. 2007; Bacteria interfere with A. actinomycetemcomitans colonization. J Dent Res 86:611–617 [CrossRef]
    [Google Scholar]
  23. Theilade E., Wright W., Borglum Jensen S., Loe H. 1966; Experimental gingivitis in man. II. A longitudinal clinical and microbiological investigation. J Periodont Res 1:1–13 [CrossRef]
    [Google Scholar]
  24. Van der Reijden W. A., Dellemijn-Kippuw N., Stijne-van Ness A. M., de Soet J. J., van Winkelhoff A. J. 2001; Mutans streptococci in subgingival plaque of treated and untreated patients with periodontitis. J Clin Periodontol 28:686–691 [CrossRef]
    [Google Scholar]
  25. Vapnik V. N. 1995 The Nature of Statistical Learning Theory Berlin: Springer-Verlag;
    [Google Scholar]
  26. Wiebe C. B., Putnins E. E. 2000; The periodontal disease classification system of the American Academy of Periodontology – an update. J Can Dent Assoc 66:594–597
    [Google Scholar]
  27. Zhang J. C., Zhou C., Wu B., Zhang Y. H. 2000; Investigation on interaction between Streptococcus sanguis and Porphyromonas gingivalis in specific pathogen-free rats. Chin J Dent Res 3:5–9
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47649-0
Loading
/content/journal/jmm/10.1099/jmm.0.47649-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error