@article{mbs:/content/journal/jmm/10.1099/jmm.0.47663-0, author = "Chu, Chen-Yi and Jiang, Bao-Gui and Liu, Wei and Zhao, Qiu-Min and Wu, Xiao-Ming and Zhang, Pan-He and Zhan, Lin and Yang, Hong and Cao, Wu-Chun", title = "Presence of pathogenic Borrelia burgdorferisensu lato in ticks and rodents in Zhejiang, south-east China", journal= "Journal of Medical Microbiology", year = "2008", volume = "57", number = "8", pages = "980-985", doi = "https://doi.org/10.1099/jmm.0.47663-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.47663-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "LD, Lyme disease", abstract = "A molecular epidemiological survey was conducted to investigate the presence of pathogenic Borrelia burgdorferi sensu lato (s.l.) species in the forest areas of Zhejiang province, south-east China. A total of 182 ticks of 6 species and 200 rodents of 8 species were collected and individually examined for the presence of B. burgdorferi s.l. DNA by nested PCR targeting the 5S–23S rRNA intergenic spacer. Forty-one ticks of four species, Haemaphysalis concinna, Haemaphysalis longicornis, Rhipicephalus microplus and Haemaphysalis warburconi, were infected with B. burgdorferi s.l., with an overall infection rate of 23 %. Sixteen rodents of four species, Nivivener confucianus, Nivivener coxingi, Apodemus sylvaticus and Rattus losea, were positive for B. burgdorferi s.l., with an overall prevalence of 8 %. MseI RFLP analysis and sequence analysis of the positive PCR products showed that Borrelia spirochaetes in specimens consisted of Borrelia garinii, Borrelia afzelii and Borrelia valaisiana-related group. Forty (98 %) of the B. burgdorferi s.l.-positive ticks were infected with B. garinii and one (2 %) was infected with B. afzelii. Twelve (75 %) of the positive rodents were infected with B. garinii and four (25 %) were infected with the Borrelia spirochaete belonging to B. valaisiana-related group.", }