%0 Journal Article %A Baldo, Aline %A Tabart, Jérémy %A Vermout, Sandy %A Mathy, Anne %A Collard, Alfred %A Losson, Bertrand %A Mignon, Bernard %T Secreted subtilisins of Microsporum canis are involved in adherence of arthroconidia to feline corneocytes %D 2008 %J Journal of Medical Microbiology, %V 57 %N 9 %P 1152-1156 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.47827-0 %K RFE, reconstructed interfollicular feline epidermis %K AAPF-pNa, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide %I Microbiology Society, %X Microsporum canis is a pathogenic fungus that causes a superficial cutaneous infection called dermatophytosis, mainly in cats and humans. The mechanisms involved in adherence of M. canis to epidermis have never been investigated. Here, a model was developed to study the adherence of M. canis to feline corneocytes through the use of a reconstructed interfollicular feline epidermis (RFE). In this model, adherence of arthroconidia to RFE was found to be time-dependent, starting at 2 h post-inoculation and still increasing at 6 h. Chymostatin, a serine protease inhibitor, inhibited M. canis adherence to RFE by 53 %. Moreover, two mAbs against the keratinolytic protease subtilisin 3 (Sub3) inhibited M. canis adherence to RFE by 23 %, suggesting that subtilisins, and Sub3 in particular, are involved in the adherence process. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.47827-0