1887

Abstract

SUMMARY

An antiserum-agar technique was evaluated as a method for detecting in faeces. Thirty-one laboratory strains of produced immunoprecipitate haloes during overnight growth on SS agar and blood-agar-base infusion agar (BAB) containing donkey antiserum to a vaccine strain of Other salmonella species sharing O serogroup antigens with also produced haloes when streaked in pure culture on SS-antiserum agar but not on BAB-antiserum agar. One hundred and forty-one consecutive faecal specimens were cultured on SS-antiserum agar. Results with this method were concordant with those of established isolation techniques on specimens from six of seven suspected carriers of

Ten other salmonellas were isolated from the faecal specimens but only like a serogroup-D organism, yielded false-positive haloes on antiserum agar. The antiserum-agar technique offers promise as a means of screening for in faecal cultures.

Penelope Fox provided expert technical assistance and Inelle Reynolds valuable secretarial service. Carl E. Frasch contributed several helpful insights. We are particularly indebted to John C. Feeley, Center for Disease Control, Atlanta, Georgia, for determining salmonella-antibody titres on the strain Ty 2 antiserum.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-13-2-373
1980-05-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/13/2/medmicro-13-2-373.html?itemId=/content/journal/jmm/10.1099/00222615-13-2-373&mimeType=html&fmt=ahah

References

  1. Alexander H. E., Leidy G., MacPherson C. 1946; Production of types a, b, c, d, e, and f H. influenzae antibody for diagnostic and therapeutic purposes. J. Immunol 54:207
    [Google Scholar]
  2. Bradshaw M. W., Schneerson R., Parke J. C., Robbins J. B. 1971; Bacterial antigens cross-reactive with the capsular polysaccharide of Haemophilus influenzae type b. Lancet 1:1095
    [Google Scholar]
  3. Craven D. E., Frasch C. E., Mocca L. F., Rose F. B., Gonzales R. 1979; Rapid serogroup identification of Neisseria meningitidis by using antiserum-agar: prevalence of serotypes in a disease-free military population. J. clin. Microbiol 10:302
    [Google Scholar]
  4. Craven D. E., Frasch C. E., Robbins J. B., Feldman H. A. 1978; Serogroup identification of Neisseria meningitidis: comparison of an antiserum agar method with bacterial slide agglutination. J. clin. Microbiol., 7, 410. Division of Immunology, Walter Reed Army Institute of Research. 1964. Preparation of dried acetone-inactivated and heat-phenol-inactivated typhoid vaccines. Bull. Wld Hlth Org 30:635
    [Google Scholar]
  5. Ewing W. H., Martin W. J. 1974; Enterobacteriaceae. In Manual of clinical microbiology 2nd ed., edited by Lennette E. H. Spaulding E. H. Traunt J. P. American Society for Microbiology; Washington, DC.: p 204
    [Google Scholar]
  6. Nolan C. M., Rosenfeld J. 1977; Antibiotic susceptibility of Salmonella typhi from chronic enteric carriers. Curr. ther. Res 21:736
    [Google Scholar]
  7. Petrie G. F. 1932; A specific precipitin reaction associated with the growth on agar plates of meningococcus, pneumococcus, and B. dysenteriae (Shiga). Br. J. exp. Path 13:380
    [Google Scholar]
  8. Schneerson R., Bradshaw M., Whisnant J. K., Myerowitz R. L., Parke J. C. Jr, Robbins J. B. 1972; An Escherichia coli antigen cross-reactive with the capsular polysaccharide of Haemophilus influenzae type b: occurrence among known serotypes, and immunochemical and biologic properties of E. coli antisera toward H. influenzae type b. J. Immunol 108:1551
    [Google Scholar]
  9. Siegel S. 1956; Non-parametric statistics for the behavioral sciences. McGraw-Hill Book Co.; New York: p 101
    [Google Scholar]
  10. Sivonen A., Renkonen O.-V., Robbins J. B. 1977; Use of antiserum agar plates for serogrouping of meningococci. J. clin. Path 30:834
    [Google Scholar]
  11. Wassen A. 1930; Sur une methode d’enrichissement des Bacilles paratyphiques, basee sur la mobilite et l’agglutination directe des Bacilles dans le milieu. C. r. Seanc. Soc. Biol 104:523
    [Google Scholar]
  12. Wong K. H., Feeley J. C. 1972; Isolation of Vi antigen and a simple method for its measurement. Appl. Microbiol 24:628
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-13-2-373
Loading
/content/journal/jmm/10.1099/00222615-13-2-373
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error