1887

Abstract

PLATE XL

, which is usually associated with mastitis in cattle, has been reported as a cause of sore throat and tonsillitis in man (Jebb, 1948; Saxholm, 1951; Tomlinson, 1966). Subcutaneous injection of this organism into guinea-pigs produces features, such as haemorrhage and oedema at the site of injection and haemorrhagic adrenals, which are characteristic of diphtheria toxin. Diphtheria antitoxin neutralises the lethal effect of toxin from some strains of (Fox and Frost, 1974) but not from others (Souckova and Soucek, 1974). In this study the purification and preliminary characterisation of a toxin from strain 378 is reported.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-13-4-587
1980-11-01
2024-04-18
Loading full text...

Full text loading...

References

  1. Baseman J. B., Pappenheimer A. M. Jr, Gill D. M., Harper A. A. 1970; Action of diphtheria toxin in the guinea-pig. J. exp. Med 132:1138
    [Google Scholar]
  2. Collier R. J., Kandel J. 1971; Structure and activity of diphtheria toxin. I. Thiol-dependent dissociation of a fraction of toxin into enzymically active and inactive fragments. J. biol. Chem 246:1496
    [Google Scholar]
  3. Crowle A. J. 1961 Immunodiffusion Academic Press; New York: p 304
    [Google Scholar]
  4. Fox J. G., Frost W. W. 1974; Corynebacterium ulcerans mastitis in a bonnet macaque (Macaca radiata). Lab. anim. Sci 24:820
    [Google Scholar]
  5. Gill D. M., Dinius L. L. 1971; Observation on the structure of diphtheria toxin. J. biol. Chem 246:1485
    [Google Scholar]
  6. Jebb W. H. H. 1948; Starch-fermenting gelatin liquefying corynebacteria isolated from the human nose and throat. J. Path. Bad 60:403
    [Google Scholar]
  7. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  8. Sargent J. R., George S. G. 1975 Methods in zone electrophoresis 3rd ed BDH Chemical Ltd; Poole: p 109
    [Google Scholar]
  9. Saxholm R. 1951; Toxin-producing diphtheria-like organisms isolated from cases of sore throat. J. Path. Bad 63:303
    [Google Scholar]
  10. Souckova A., Soucek A. 1974; Two distinct toxic proteins in Corynebacterium ulcerans. J. Hyg. Epidem. Microbiol. Immun 18:336
    [Google Scholar]
  11. Tomlinson A. J. H. 1966; Human pathogenic coryneform bacteria: their differentiation and significance in public health today. J. appl. Bad 29:131
    [Google Scholar]
  12. Weber K., Osborn M. 1969; The reliability of molecular weight determination by dodecyl sulphate polyacrylamide-gel electrophoresis. J. biol. Chem 244:4406
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-13-4-587
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error