1887

Abstract

SUMMARY

We used selective media together with aerobic and anaerobic incubation for the quantitation of common pathogens in liquefied sputum from children with cystic fibrosis. The accuracy of the technique was verified by reconstruction studies in which laboratory strains with antibiotic-resistance markers were added to sputum from cystic fibrosis patients. Comparison of the numbers of bacteria found on quantitative culture of clinical specimens with the “predominant” organism found on routine culture yielded a poor correlation. When was the most prevalent on routine culture, it was present in the highest numbers on quantitative culture (mean count = 10 cfu/g). However, large numbers of (mean count = 10 cfu/g), (mean count = 2 x 10 cfu/g), and streptococci (mean count = 2 x 10 cfu/g) were also present in these cultures. When was the predominant organism, and were also present in similar numbers ( 10 cfu/g). When was the predominant species on routine culture, the mean count was 7 x 10 cfu/g and was often completely absent. We conclude that the selective technique permits reliable enumeration of sputum bacteria, and offers a more accurate assessment of the microbial flora of sputum in cystic fibrosis than does simple plating of unhomogenised sputum.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-17-2-113
1984-04-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jmm/17/2/medmicro-17-2-113.html?itemId=/content/journal/jmm/10.1099/00222615-17-2-113&mimeType=html&fmt=ahah

References

  1. Bartlett J. G., Finegold S. M. 1978; Bacteriology of expectorated sputum with quantitative culture and wash technique compared to trans-tracheal aspirates. American Review of Respiratory Disease 117:1019–1027
    [Google Scholar]
  2. Feigelson J., Pecau Y. 1967; Bacteriological studies of bronchial secretions in 19 cases of cystic fibrosis. Modern Problems in Pediatrics 10:214–226
    [Google Scholar]
  3. George R. H., Gillett A. P., Roycroft C. A. 1980; Haemophilus infections in cystic fibrosis. Lancet 2:262
    [Google Scholar]
  4. Hammerschlag M. R., Harding L., Macone A., Smith A. L., Goldmann D. A. 1980; Bacteriology of sputum in cystic fibrosis: Evaluation of dithiothreitol as a mucolytic agent. Journal of Clinical Microbiology 11:552–557
    [Google Scholar]
  5. Huang N. N., Van Loon E. L., Sheng K. T. 1961; The flora of the respiratory tract of patients with cystic fibrosis of the pancreas. Journal of Pediatrics 59:512–521
    [Google Scholar]
  6. Iacocca V. F., Sibinga M., Barbero G. J. 1963; Respiratory tract bacteriology in cystic fibrosis. American Journal of Diseases of Children 106:315–324
    [Google Scholar]
  7. Kilbourn J. P., Campbell R. A., Grach J. L., Willis M. D. 1968; Quantitative bacteriology of sputum. American Review of Respiratory Disease 98:810–818
    [Google Scholar]
  8. Lapinski E. M., Flakas E. D., Taylor B. C. 1964; An evaluation of some methods for culturing sputum from patients with bronchitis and emphysema. American Review of Respiratory Disease 89:760–763
    [Google Scholar]
  9. Lederberg J., Lederberg E. M. 1952; Replica plating and indirect selection of bacterial mutants. Journal of Bacteriology 63:399–406
    [Google Scholar]
  10. Lennette E. H., Balows A., Hausler W. J., Truant J. P. 1980 Manual of clinical microbiology 3rd edn American Society for Microbiology; Washington DC:
    [Google Scholar]
  11. Louria D. B. 1962; Uses of quantitative analyses of bacterial populations in sputum. Journal of the American Medical Association 182:1082–1086
    [Google Scholar]
  12. May J. R. 1953; The bacteriology of chronic bronchitis. Lancet 2:534–537
    [Google Scholar]
  13. May J. R., Herrick N. C., Thompson D. 1972; Bacterial infection in cystic fibrosis. Archives of Disease in Childhood 47:908–913
    [Google Scholar]
  14. Monroe P. W., Muchmore H. G., Felton F. G., Pirtle J. K. 1969; Quantitation of microorganisms in sputum. Applied Microbiology 18:214–220
    [Google Scholar]
  15. Pecora D. V. 1959; A method of securing uncontaminated tracheal secretions for bacterial examination. Journal of Thoracic Surgery 37:653–654
    [Google Scholar]
  16. Roberts D. E., Cole P. 1980; Use of selective media in bacteriological investigation of patients with chronic suppurative respiratory infection. Lancet 1:796–797
    [Google Scholar]
  17. Sondag J. E., Morgens R. K., Hoppe J. E., Marr J. J. 1977; Detection of pneumococci in respiratory secretions: Clinical evaluation of gentamicin blood agar. Journal of Clinical Microbiology 5:397–400
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-17-2-113
Loading
/content/journal/jmm/10.1099/00222615-17-2-113
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error