1887

Abstract

Summary

Calcium-dependent binding of C-reactive protein (CRP) to was determined by enzyme-linked immunosorbent assay. A homogenate of young hyphae was fractionated by hydrophobic interaction chromatography followed by gel filtration. High CRP-binding activity was found in a fraction of mol. wt 500,000 which was characterised by strong binding to the hydrophobic column. Three fractions of less conspicuous CRP-binding activity were identified ( 500 000, 150 000 and 150 000−50 000 mol. wt respectively). In these four fractions, phosphorylcholine was detected by an anti-phosphorylcholine mouse hybridoma antibody. Some CRP-binding activity in fractions with low affinity for the hydrophobic column did not correspond closely with the presence of phosphorylcholine. It is suggested that C-reactive substance in is heterogeneous. The C-reactive substances did not correspond with fractions containing major antigens (470 000 and 250 000 mol. wt respectively) which elicit a strong immune response in man.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-21-2-173
1986-03-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jmm/21/2/medmicro-21-2-173.html?itemId=/content/journal/jmm/10.1099/00222615-21-2-173&mimeType=html&fmt=ahah

References

  1. Baldo B. A., Fletcher T. C., Pepys J. 1977; Isolation of a peptido-polysaccharide from the dermatophyte Epidermophyton fioccosum and a study of its reaction with human C-reactive protein and a mouse anti-phosphorylcholine myeloma serum. Immunology 32:831–842
    [Google Scholar]
  2. DiCamelli R., Potempa L. A., Siegel J., Suyehira L., Petras K., Gewurz H. 1980; Binding reactivity of C-reactive protein for polycations. Journal of immunology 125:1933–1938
    [Google Scholar]
  3. Eaton N. R. 1962; New Press for disruption of microorganisms. Journal of Bacteriology 83:1359–1360
    [Google Scholar]
  4. Gotschlich E. C., Edelman G. M. 1967; Binding properties and specificity of C-reactive protein. Proceedings of the National Academy of Sciences of the USA 57:706–712
    [Google Scholar]
  5. Gotschlich E. C., Liu T. Y., Oliveira E. 1982; Binding of C-reactive protein to C-carbohydrate and PC-substituted protein. Annals of the New York Academy of Science 389:163–171
    [Google Scholar]
  6. Johnson U., Preliner K. 1977; Purification of C-reactive protein on DEAE-cellulose by a simple twostep procedure utilizing the calcium-dependency of the protein. Biochimica et Biophy-sica Acta 495:349–353
    [Google Scholar]
  7. Kaplan M. H., Volanakis J. E. 1974; Interaction of C-reactive protein complexes with the complement system. I. Consumption of human complement associated with the reaction of C-reactive protein with pneumococcal C-polysac-charide and with the choline phosphatides, lecithin and sphingomyelin. Journal of Immunology 112:2135–2147
    [Google Scholar]
  8. Longbottom J. L., Pepys J. 1964; Pulmonary aspergillosis: Diagnostic and immunological significance of antigens and C-substance in Aspergillusfumigatus. Journal of Pathology and Bacteriology 88:141–151
    [Google Scholar]
  9. Pennington J. E. 1980; Aspergillus lung disease. Medical Clinics of North America. 64:475–490
    [Google Scholar]
  10. Pepys J., Longbottom J. L. 1971; Antigenic and C-substance activities of related glycopeptides from fungal, parasitic and vegetable sources. International Archives of Allergy and Applied Immunology 41:219–221
    [Google Scholar]
  11. Robey F. A., Jones K. D., Tanaka T., Liu T. Y. 1984; Binding of C-reactive protein to chromatin and nucleosome core particles. A possible physiological role of C-reactive protein. Journal of Biological Chemistry 259:7311–7316
    [Google Scholar]
  12. Schonheyder H., Andersen P. 1984a; Fractionation of Aspergillus fumigatus antigens by hydrophobic interaction chromatography and gel filtration. International Archives of Allergy and Applied Immunology 73:231–236
    [Google Scholar]
  13. Schonheyder H., Andersen P. 1984b; IgG antibodies to purified Aspergillus fumigatus antigens determined by enzyme-linked immunosorbent assay. International Archives of Allergy and Applied Immunology 74:262–269
    [Google Scholar]
  14. Sorensen U. B. S., Agger R., Bennedsen J., Henrichsen J. 1984; Phosphorylcholine determinants in six pneumococcal capsular polysaccharides detected by monoclonal antibody. Infection and Immunity 43:876–878
    [Google Scholar]
  15. Volanakis J. E., Kaplan M. H. 1971; Specificity of C-reactive protein for choline phosphate residues of pneumococcal C-polysaccharide. Proceedings of the Society for Experimental Biology and Medicine 136:612–614
    [Google Scholar]
  16. Voiler A., Bidwell D. E., Bartlett A. 1976; Enyzme immunoassays in diagnostic medicine. Theory and practice. Bulletin of the World Health Organization 53:55–65
    [Google Scholar]
  17. Weeke B. 1973; Crossed immunoelectrophoresis. Scandinavian Journal of Immunology Suppl. 1:47–56
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-21-2-173
Loading
/content/journal/jmm/10.1099/00222615-21-2-173
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error