1887

Abstract

Summary

Direct plating of faecal specimens on blood agar was compared with the use of enrichment culture for isolation of spp. from faeces during a large epidemiological study. Of enterotoxigenic strains isolated by direct plating, 89% were associated with acute diarrhoea and 7% with an episode of diarrhoea during the month before collection, but 79% of enterotoxigenic strains isolated only after enrichment were not associated with acute diarrhoea. With spp., as with intestinal pathogens, it appears that enrichment allows isolation of the bacteria when in low faecal concentrations likely to be found in convalescent patients, carriers and those with subclinical infection. The routine use of enrichment for isolation of faecal aeromonads, by detecting spp. in low numbers in patients without diarrhoea, is likely to confuse interpretation of epidemiological studies seeking to clarify the relationship between spp. and acute diarrhoea.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-22-4-315
1986-12-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jmm/22/4/medmicro-22-4-315.html?itemId=/content/journal/jmm/10.1099/00222615-22-4-315&mimeType=html&fmt=ahah

References

  1. Burke Gracev M. 1981; Detection of enterotoxins of Aeromonas hydrophila by a suckling mouse test. Journal of Medical Microbiology 14:401–408
    [Google Scholar]
  2. Cowan S. T. 1974; Cowan and Steel’s Manual for identification of medical bacteria. The Cambridge University Press; Cambridge:
    [Google Scholar]
  3. Echeverria P., Blacklow N. R., L B., Cukor G. G. 1981; Traveler’s diarrhea among American peace corps volunteers in rural Thailand. Journal of Infectious Diseases 143:767–771
    [Google Scholar]
  4. Furniss A. L., Lee J. V., Donovan T. J. 1978; The Vibrios. Public Health Laboratory Service Monograph Series No. 11. Her Majesty’s Stationery Office; London:
    [Google Scholar]
  5. Gangarosa E. J., Dewitt W. E., Huq I., Zarifi A. 1968; Laboratory methods in cholera: isolation of Vibrio cholerae (E1 Tor and classical) on TCBS medium in minimally equipped laboratories. Transactions of the Royal Society of Tropical Medicine and Hygiene 62:693–699
    [Google Scholar]
  6. Gracey M., Burke V., Robinson J. 1982; Aeromonas-associated gastroenteritis. Lancet 2:1304–1306
    [Google Scholar]
  7. Kaper J., Seidler R. J., Lockman H., Colwell R. R. 1979; Medium for the presumptive identification of Aeromonas hydrophila and Enterobacteriacae. Applied and Environmental Microbiology 38:1023–1026
    [Google Scholar]
  8. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703
    [Google Scholar]
  9. Millership S. E., Chattopadhyay B. 1984; Methods for the isolation of Aeromonas hydrophila and Plesiomonas shigelloides from faeces. Journal of Hygiene 92:145–152
    [Google Scholar]
  10. Millership S. E., Chattopadhyay B. 1983; Faecal carriage rate of Aeromonas hydrophila. Journal of Clinical Pathology 36:920–923
    [Google Scholar]
  11. Moulsdale M. T. 1983; Isolation of Aeromonas from faeces. Lancet 1:351
    [Google Scholar]
  12. Pitarangsi C. 1982; Enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigelloides: prevalence among individuals with and without diarrhea in Thailand. Infection and Immunity 35:666–673
    [Google Scholar]
  13. Popoff M. 1984; Aeromonas Kluyver and Van Niel. In Krieg N. R., Holt J. G. (eds) Bergey’s Manual of systematic bacteriology 9th edn, vol 1 Williams and Wilkins Co; Baltimore: pp 545–548
    [Google Scholar]
  14. Price E. H., Hunt G. H., Patel U., Smith J. A. 1984; Aeromonas spp. in diarrhoea. British Medical Journal 289:1380
    [Google Scholar]
  15. Rennels M. B., Levine M. M., Daya V., Angle P., Young C. 1980; Selective vs. non selective media and direct plating vs enrichment technique in isolation of Vibrio cholerae: recommendations for clinical laboratories. Journal of Infectious Diseases 142:328–331
    [Google Scholar]
  16. Robinson J., Burke V., Worthy P. J., Beaman J., Wagener L. 1984; Media for isolation of Aeromonas spp. from faeces. Journal of Medical Microbiology 18:405–411
    [Google Scholar]
  17. Rogol M., Sechter I., Grinberg L., Gerichter C. B. 1979; Pril-xylose-ampicillin agar, a new selective medium for the isolation of Aeromonas hydrophila. Journal of Medical Microbiology 12:229–231
    [Google Scholar]
  18. Shread P., Donovan T. J., Lee J. V. 1981; A survey of the incidence of Aeromonas in human faeces. Society for General Microbiology Quarterly 8:184
    [Google Scholar]
  19. VonGraevenitz A., Bucher C. 1983; Evaluation of differential and selective media for isolation of Aeromonas and Plesiomonas spp. from human feces. Journal of Clinical Microbiology 17:16–21
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-22-4-315
Loading
/content/journal/jmm/10.1099/00222615-22-4-315
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error