1887

Abstract

Summary.

The production of toxic shock syndrome toxin-1 (TSST-1) was studied in batch and continuous culture of strain 1169 in a carbohydrate-free chemically defined medium (CDM). In continuous culture oxygen- and arginine-limitation were required for steady-state TSST-1 synthesis. Aeration suppressed toxin synthesis. The amount of TSST-1 per mg dry weight (specific toxin) at dilution rates from 0·05 to 0·15 h was inversely proportional to the dilution rate. Protease activity increased with increasing dilution rates. In batch culture, TSST-1 began to accumulate in the medium towards the end of the exponential phase of growth, after a critical cell mass was attained. Maximum specific toxin production was observed in medium with an initial H between 6·5 and 7·0. Growth and toxin synthesis took place in anaerobic conditions when CDM was supplemented with pyruvate and uracil. The Mg concentration had no effect on the specific toxin in anaerobic conditions. In aerobic conditions, specific toxin increased . 23-fold as the Mg concentrations increased to 0·4 m. Further increases in the Mg concentration resulted in a reduction in specific toxin.

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1987-08-01
2024-04-19
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References

  1. Arko R J, Rasheed J K, Broome C V, Chandler F W, Paris A L. 1984; A rabbit model of toxic shock syndrome: clinicopath-ological features. Journal of Infection 8:205–211
    [Google Scholar]
  2. Beller F K, Schweppe K W. 1979; Review on the biology of menstrual blood. Beller F K, Schumacher G F B. The biology of the fluids of the female genital tract Elsevier; North-Holland, New York:231–245
    [Google Scholar]
  3. Blake M S, Johnston K H, Russell-Jones G J, Gotschlich E C. 1984; A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Analytical Biochemistry 136:175–179
    [Google Scholar]
  4. Carpenter D F, Silverman G J. 1974; Staphylococcal enterotoxin B and nuclease production under controlled dissolved oxygen conditions. Applied Microbiology 28:628–637
    [Google Scholar]
  5. Catlin B W. 1973; Nutritional profiles of Neisseria gonorrhoeae, Neisseria meningitidis and Neisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing. Journal of Infectious Diseases 128:178–194
    [Google Scholar]
  6. Crass B A, Bergdoll M S. 1986; Toxin involvement in toxic shock syndrome. Journal of Infectious Diseases 153:918–926
    [Google Scholar]
  7. Engels W, Kamps M A F, Van Boven C P A. 1980; Continuous culture studies on the production of staphylocoagulase by Staphylococcus aureus . Antonie van Leeuwenhoek 46:533–550
    [Google Scholar]
  8. Engels W, Kamps M A F. 1982; The nature of the competitive ability of spontaneous staphylocoagulase-negative mutants of Staphylococcus aureus with respect to growth of the parent strains in continuous culture. Antonie van Leeuwenhoek 48:67–83
    [Google Scholar]
  9. Friedemann T E. 1939; The carbohydrate metabolism of Staphylococcus aureus. Journal of Biological Chemistry 130:61–65
    [Google Scholar]
  10. Jarvis A W, Lawrence R C, Pritchard G G. 1973; Production of staphylococcal enterotoxins A, B and C under conditions of controlled pH and aeration. Infection and Immunity 7:847–854
    [Google Scholar]
  11. Jarvis A W, Lawrence R C, Pritchard G G. 1975; Glucose repression of enterotoxins A, B and C and other extracellular proteins in staphylococci in batch and continuous culture. Journal of General Microbiology 86:75–87
    [Google Scholar]
  12. Laemmli U K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  13. Lowry O H, Rosebrough N J, Farr A L, Randall R J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  14. Mancini G, Carbonara A O, Heremans J F. 1965; Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2:235–254
    [Google Scholar]
  15. Maw J, Meynell G G. 1968; The true division and death rates of Salmonella typhimurium in the mouse spleen determined with superinfecting phage P22. British Journal of Experimental Pathology 49:597–613
    [Google Scholar]
  16. Mietzner T A, Luginbuhl G H, Sandstrom E, Morse S A. 1984; Identification of an iron-regulated 37,000-dalton protein in the cell envelope of Neisseria gonorrhoeae. Infection and Immunity 45:410–416
    [Google Scholar]
  17. Mills J T, Parsonnet J, Tsai Y-C, Kendrick M, Hickman R K, Kass E H. 1985; Control of production of toxic-shock-syndrome toxin-1 (TSST-1) by magnesium ion. Journal of Infectious Diseases 151:1158–1161
    [Google Scholar]
  18. Mills J T, Parsonnet J, Kass E H. 1986; Production of toxic-shock-syndrome toxin-1: effect of magnesium ion. Journal of Infectious Diseases 153:993–994
    [Google Scholar]
  19. Morse S A, Baldwin J N. 1973; Factors affecting the regulation of staphylococcal enterotoxin B. Infection and Immunity 7:839–846
    [Google Scholar]
  20. Morse S A, Stein S, Hines J. 1974; Glucose metabolism in Neisseria gonorrhoeae. Journal of Bacteriology 120:702–714
    [Google Scholar]
  21. Polk H C, Miles A A. 1973; The decisive period in the primary infection of muscle by Escherichia coli. British Journal of Experimental Pathology 54:99–109
    [Google Scholar]
  22. Rakoff A E, Feo L G, Goldstein L. 1944; The biologic characteristics of the normal vagina. American Journal of Obstetrics and Gynecology 47:467–494
    [Google Scholar]
  23. Reeves M W, Pine L, Feeley J C, Wells D E. 1984; Presence of toxic shock toxin in toxic shock and other clinical strains of Staphylococcus aureus. Infection and Immunity 46:590–597
    [Google Scholar]
  24. Richardson G M. 1936; The nutrition of Staphylococcus aureus. Necessity for uracil in anaerobic growth. Biochemical Journal 30:2184–2190
    [Google Scholar]
  25. Rinderknecht H, Geokas M C, Silverman P, Haverback B J. 1968; A new ultrasensitive method for the determination of proteolytic activity. Clinica Chimica Acta 21:197–203
    [Google Scholar]
  26. Schlievert P M, Blomster D A. 1983; Production of staphylococcal pyrogenic exotoxin Type C: influence of physical and chemical factors. Journal of Infectious Diseases 147:236–242
    [Google Scholar]
  27. Schlievert P M. 1985; Effect of magnesium on production of toxic-shock-syndrome toxin-1 by Staphylococcus aureus. Journal of Infectious Diseases 152:618–620
    [Google Scholar]
  28. Scott D F, Kling J M, Kirkland J J, Best G K. 1983; Characterization of Staphylococcus aureus isolates from patients with toxic shock syndrome, using polyethylene infection chambers in rabbits. Infection and Immunity 39:383–387
    [Google Scholar]
  29. Spackman D H, Stein W H, Moore S. 1958; Automatic recording apparatus for use in the chromatography of amino acids. Analytical Chemistry 30:1190–1206
    [Google Scholar]
  30. Todd J, Fishaut M, Kapral F, Welch T. 1978; Toxic-shock syndrome associated with phage-group-I staphylococci. Lancet 2:1116–1118
    [Google Scholar]
  31. Towbin H, Staehelin T, Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the USA 76:4350–4354
    [Google Scholar]
  32. Wagner G, Bohr L, Wagner P, Petersen L N. 1984; Tampon-induced changes in vaginal oxygen and carbon dioxide tensions. American Journal of Obstetrics and Gynecology 148:147–150
    [Google Scholar]
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