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Volume 30, Issue 1

Humoral Immunity in mice Mediated by Monoclonal Antibodies Against the A and M Antigens of Free

Abstract

Summary

All smooth strains of bear two lipopolysaccharide (LPS) antigens in a ratio that defines the classification of strains in serovars, A (A≥M), M (M>A) and A.M (A = M). Anti-LPS-A monoclonal antibodies (MAb-A) were previously shown to convey protection to mice against (A) strain 544, as shown by lower spleen counts than in controls at days 7 and 21 after challenge. Anti-LPS-M monoclonal antibodies (MAb-M) were obtained and tested for M-specificity with LPS from reference strains by ELISA, by agglutination of LPS-coated latex particles, and by inhibition of this agglutination. Antigens A and M of three strains were quantified by a homologous LPS-latex and MAb agglutination inhibition assay. Protection conferred by MAb-A and MAb-M against three strains, 544 (A), 292 (M) and H38 (M), was tested at equivalent challenge and MAb doses: intravenous challenge was adjusted to give similar infection at day 7;MAb doses were adjusted to the same specific ELISA titre. Under these conditions, MAb-A and MAb-M conferred both early and late protection, as shown at days 7 and 21, against the strains that bore the homologous major antigen, , strain 544 on one hand and strains H38 and 292 on the other. In contrast, MAb directed against the minor antigen of the challenge strain conferred significant protection at day 7 only with strains 544 and H38 and no or inconsistent protection against strain 292, which expressed the lowest amount of minor antigen. Thus, early and late antibody-mediated immune mechanisms depend on amounts of surface LPS antigens accessible to specific antibodies. Therefore, to protect against the various strains of , an LPS-based vaccine should induce high litres of specific antibodies against both A and M antigens.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1989
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1989-09-01
2024-04-18
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References

  1. Baker P. J., Wilson J. B. 1965; Chemical composition and biological properties of the endotoxin of Brucella abortus. Journal of Bacteriology 90:895–902
     [Google Scholar]
  2. Bosseray N., Plommet M., De Rycke J. 1982; Evolution de l’infection de la souris par Brucella abortus, Brucella melitensis et Brucella suis vers l’état chronique et la guérison. Annales de Recherches Véterinaires 13:153–161
     [Google Scholar]
  3. Bosseray N., Plommet A. M., Plommet M. 1984; Theoretical, practical and statistical basis for a general control method of activity for anti-Brucella vaccines. Developments in Biological Standardization 56:257–270
     [Google Scholar]
  4. Dubray G. 1987; Protective antigens in brucellosis. Annales de l’Institut Pasteur/Microbiologie 138:84–87
     [Google Scholar]
  5. Dubray G., Limet J. N. 1987; Evidence of heterogeneity of lipopolysaccharides among Brucella biovars in relation to A and M specificities. Annales de l’Institut Pasteur/Microbiologie 138:27–37
     [Google Scholar]
  6. Limet J. N., Berbinschi A. C., Cloeckaert A., Cambiaso C. L., Masson P. L. 1988; Longitudinal study of brucellosis in mice by immunoassay of lipopolysaccharide-related antigens in blood and urine. Journal of Medical Microbiology 26:37–45
     [Google Scholar]
  7. Limet J. N., Plommet A. M., Dubray G., Plommet M. 1987; Immunity conferred upon mice by anti-LPS monoclonal antibodies in murine brucellosis. Annales de l’Institut Pasteur/Immunologie 138:417–424
     [Google Scholar]
  8. Mackaness G. B. 1964; The immunological basis of acquired cellular resistance. Journal of Experimental Medicine 120:105–120
     [Google Scholar]
  9. Masson P. L., Cambiaso C. L., Collet-Cassart D., Magnusson C. G. M., Richards C. B., Sindic C. J. M. 1981; Particle counting immunoassay (PACIA). Methods in Enzymology 14:106–139
     [Google Scholar]
  10. Montaraz J. A., Winter A. J., Hunter D. M., Sowa B. A., Wu A. M., Adams L. G. 1986; Protection against Brucella abortus in mice with O-polysaccharide-specific monoclonal antibodies. Infection and Immunity 51:961–963
     [Google Scholar]
  11. Pavlov H., Hogarth M., McKenzie I. F. C., Cheers C. 1982; In vivo and in vitro effects of monoclonal antibody to Ly antigens on immunity to infection. Cellular Immunology 71:127–138
     [Google Scholar]
  12. Plommet M., Plommet A. M. 1983; Immune serum-mediated effects on brucellosis evolution in mice. Infection and Immunity 41:97–105
     [Google Scholar]
  13. Plommet M., Plommet A. M. 1987; Anti-Brucella cell-mediated immunity in mice vaccinated with a cell wall fraction. Annales de Recherches Véterinaires 18:429–437
     [Google Scholar]
  14. Ralston D. J., Elberg S. S. 1971; The cell wall as the antigenic site for antibodies stimulating ingestion (MSF) and inhibition (BIF) of Brucella in macrophages from normal and immune animals. British Journal of Experimental Pathology 52:130–137
     [Google Scholar]
  15. Renoux G. 1955; Etudes sur la Brucella ovine et caprine. I. Choix d’une souche infectante de B. melitensis. Archives de llnstitut Pasteur de Tunis 32:51–64
     [Google Scholar]
  16. Shulman M., Wilde C. D., Köhler G. 1978; A better cell line for making hybridomas secreting specific antibodies. Nature 276:269–270
     [Google Scholar]
  17. Stableforth A. W., Jones L. M. 1963; Report of the subcommittee on taxonomy of the genus Brucella. Speciation in the genus Brucella. International Bulletin of Bacteriological Nomenclature and Taxonomy 13:145–158
     [Google Scholar]
  18. Steel R. G. D., Torrie J. H. 1960; Principles and procedures of statistics, with special reference to biological sciences. McGraw-Hill Book Company, Inc; New York: pp 99–160
     [Google Scholar]
  19. Sulitzeanu D., Jones L., Stableforth A. W. 1955; Protective action of monospecific anti-Brucella sera in mice. Nature 175:1040–1041
     [Google Scholar]
  20. Van Snick J. L., Coulie P. 1982; Monoclonal anti-IgG antibodies derived from lipopolysaccharide-activated spleen cells of 129/SV mice. Journal of Experimental Medicine 155:219–230
     [Google Scholar]
  21. Vendrell J. P., Delobbe A., Hughuet M. F., Peraldi F., Serre A., Cannat A. 1987; Biological properties of a panel of murine monoclonal anti-Brucella antibodies. Immunology 61:7–10
     [Google Scholar]
  22. Wilson G. S., Miles A. A. 1932; The serological differentiation of smooth strains of the Brucella group. British Journal of Experimental Pathology 13:1–13
     [Google Scholar]
  23. Winter A. J. 1987; Outer membrane proteins of Brucella. Annales de l’Institut Pasteur/Microbiologie 138:87–89
     [Google Scholar]
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Humoral Immunity in mice Mediated by Monoclonal Antibodies Against the A and M Antigens of Brucella
J. Med. Microbiol. 30, 37 (1989); https://doi.org/10.1099/00222615-30-1-37
/content/journal/jmm/10.1099/00222615-30-1-37
/content/journal/jmm/10.1099/00222615-30-1-37
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