1887

Abstract

Summary

Nitrogen mustard (NM) treatment of rabbits induced neutropenia, and, in ligated ileal loops, it inhibited fluid secretion induced by salmonella or by cholera toxin (CT). Pretreatment of rabbits with indomethacin almost abolished salmonella-induced fluid secretion and significantly reduced that induced by CT. Similar effects of NM and indomethacin on fluid secretion induced by salmonella, but not by CT, have been reported by other workers and used to implicate prostaglandins, from the salmonella-induced inflammation, as mediators of fluid secretion. In contrast, we show that NM treatment, in addition to reducing CT-induced secretion, caused severe morphological alterations to ileal mucosa. Irradiation techniques were developed for inducing neutropenia, but they did not totally inhibit salmonella-induced leucocyte influx into ileal mucosa. We propose an alternative mechanism for the inhibitory effect of NM on salmonella- and CT-induced secretion, based on the known anti-mitotic activity of NM. Also, the anti-secretory effect of indomethacin cannot be attributed uniquely to its anti-inflammatory activity because it depressed CT-induced secretion as well as salmonella-induced secretion. These results support the concept of pathophysiological secretion in infectious diarrhoea, developed previously for rotavirus and extended to bacterial infections.

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1990-01-01
2024-03-28
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References

  1. Clarke G J et al. 1988; Expression of an antigen in strains of Salmonella typhimurium which reacts with antibodies to cholera toxin. Journal of Medical Microbiology 25:139–146
    [Google Scholar]
  2. Collins J et al. 1988; Intestinal enzyme profiles in normal and rotavirus-infected mice. Journal of Pediatric Gastroenterology and Nutrition 7:264–272
    [Google Scholar]
  3. Duebbert I E, Peterson J W. 1985; Enterotoxin-induced fluid accumulation during experimental salmonellosis and cholera: involvement of prostaglandin synthesis by intestinal cells. Toxicon 23:157–172
    [Google Scholar]
  4. mmanuel F X S, Vaughan ATM, Catty D. 1981; Mice treated with Strontium-90: an animal model deficient in NK cells. British Journal of Cancer 44:160–165
    [Google Scholar]
  5. Field M, Smith P L, Bolton J E. 1980; Ion transport across the isolated intestinal mucosae of the winter flounder, Pseudo-pleuronectes americanus II; effects of cyclic AMP. Journal of Membrane Biology 55:157–163
    [Google Scholar]
  6. Giannella R A. 1979; The importance of the intestinal inflammatory reaction in Salmonella-mediated intestinal secretion. Infection and Immunity 23:140–145
    [Google Scholar]
  7. Gots R E, Formal S B, iannella R A. 1974; Indomethacin inhibition of Salmonella typhimurium, Shigella flexneri and cholera-mediated rabbit ileal secretion. Journal of Infectious Diseases 130:280–284
    [Google Scholar]
  8. Kimberg D V, Field M, Gershon E, Schooley R T, Henderson A. 1973; Effects of cycloheximide on the response of the intestinal mucosa to cholera enterotoxin. Journal of Clinical Investigation 52:1376–1383
    [Google Scholar]
  9. O'Mara R E, Cafee J G, Subramanian G. 1969; Rare earth nuclides as potential agents for skeletal imaging. Journal of Nuclear Medicine 10:49–51
    [Google Scholar]
  10. Peterson J W, Berg W D, Ochoa L G. 1988; Indomethacin inhibits cholera toxin-induced cyclic AMP accumulation in Chinese hamster ovary cells. FEMS Microbiology Letters 49:187–192
    [Google Scholar]
  11. Roggin G M, Banwell J G, Yardley J H, Hendrix T R. 1972; Unimpaired response of rabbit jejunum to cholera toxin after selective damage to villus epithelium. Gastroenterology 63:81–989
    [Google Scholar]
  12. Salmon S E. 1980; Cancer Chemotherapy. In: Meyers F H et al. (eds) Review of medical pharmacology, 7th edn.. Lange Medical Publications; Los Altos, CA: pp 477–513
    [Google Scholar]
  13. Spencer A J et al. X-ray microanalysis of rotavirus-infected mouse intestine: a new concept of diarrhoeal secretion.. Journal ofPaediatric Gastroenterology and Nutrition in press
    [Google Scholar]
  14. Stephen J, Osborne M P. 1988; Pathophysiological mechanisms in diarrhoeal disease. In Donachie W. (eds) Bacterial infections of the respiratory and gastrointestinal mucosae SGM Special publication Vol 24 IRL Press; Oxford: pp 149–170
    [Google Scholar]
  15. Stephen J, Pietrowski R A. 1986; Bacterial toxins. , 2nd edn. Van Nostrand Reinhold (UK), Wokingham, UK:
    [Google Scholar]
  16. Vaughan ATM, Anderson P, Dykes P W, Chapman C E, Bradwell A R. 1987; Limitations to the killing of tumours using radiolabeled antibodies. British Journal of Radiology 60:567–572
    [Google Scholar]
  17. Wallis T S et al. 1989; Quantification of the leucocyte influx into the rabbit ileum induced by strains of Salmonella typhimurium of different virulence. Journal of Medical Microbiology 30:49–156
    [Google Scholar]
  18. Wallis T S, Starkey W G, Stephen J, Haddon S J, Osborne M P, Candy D C A. 1986a; Enterotoxin production by Salmonella typhimurium strains of different virulence. Journal ofMedical Microbiology 2:19–23
    [Google Scholar]
  19. Wallis T S, Starkey W G, Stephen J, Haddon S J, Osborne M P, Candy D C A. 1986b; The nature and role of mucosal damage in relation to Salmonella typhimurium-mdxxced fluid secretion in the rabbit ileum. Journal of Medical Microbiology 22:39–49
    [Google Scholar]
  20. Welsh M J, Smith P L, Fromm M, Frizzell R A. 1982; Crypts are the site of intestinal fluid and electrolyte secretion. Science 218:1219–1221
    [Google Scholar]
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