1887

Journal of Medical Microbiology logo

Volume 39, Issue 1

Serum IgG and sputum IgA antibody to core lipopolysaccharide antigen from in patients with cystic fibrosis Free

Abstract

Summary

The immunological response of cystic fibrosis (CF) patients to lipopolysaccharide (LPS) antigens of was investigated. Enzyme-linked immunosorbent assays (ELISA) with either whole cells or extracted core LPS from a clinical isolate of as antigen were used to measure serum IgG and sputum IgA anti- antibodies. The ELISA with core LPS distinguished nine CF patients colonised by from nine age- and sex-matched non-colonised CF patients. The rate of increase of anti- IgG antibodies after bacteriologically proven colonisation varied in individual patients: In some patients the first isolation of was preceded or accompanied by a two-to-four-fold rise in anti- LPS IgG titres. Absorption studies and immunoblot analysis of serum from patients colonised with demonstrated that a significant component of the anti- core LPS antibodies was specific for and did not react with the core LPS of . Immunoblotting also illustrated that there may be a degree of core heterogeneity between different isolates of . Detection of LPS specific antibodies in serum (IgG) and sputum (IgA) from CF patients is recommended to assist the identification of colonisation in CF patients.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1993
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-39-1-39
1993-07-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jmm/39/1/medmicro-39-1-39.html?itemId=/content/journal/jmm/10.1099/00222615-39-1-39&mimeType=html&fmt=ahah

References

  1. Gilligan PH. Microbiology of airways disease in patients with cystic fibrosis. Clin Microbiol Rev 1991; 4:35–51
     [Google Scholar]
  2. Isles A, Maclusky I, Corey M et al. Pseudomonas cepacia in cystic fibrosis: an emerging problem. J Pediatr 1984; 104:206–210
     [Google Scholar]
  3. Simmonds EJ, Conway SP, Ghoneim ATM, Ross H, Littlewood JM. Pseudomonas cepacia : a new pathogen in patients with cystic fibrosis referred to a large centre in the United Kingdom. Arch Dis Child 1990; 65:874–877
     [Google Scholar]
  4. Govan JRW, Nelson JW. Microbiology of lung infection in cystic fibrosis. Br Med Bull 1992; 48:912–930
     [Google Scholar]
  5. Govan JRW, Nelson JW, Brown PH. Pseudomonas cepacia: an appraisal for CF. CF News 1991 Oct./Nov. 12–14
     [Google Scholar]
  6. Nelson JW, Doherty CJ, Brown PH, Greening AP, Kaufmann ME, Govan JRW. Pseudomonas cepacia in inpatients with cystic fibrosis. Lancet 1991; 338:1525
     [Google Scholar]
  7. Henderson DK, Baptiste R, Parrillo J, Gill VJ. Indolent epidemic Pseudomonas cepacia bacteremia and pseudobacteremia in an intensive care unit traced to a contaminated blood gas analyzer. Am J Med 1988; 84:75–81
     [Google Scholar]
  8. Holmes B. The identification of Pseudomonas cepacia and its occurrence in clinical material. J Appl Bacteriol 1986; 61:299–314
     [Google Scholar]
  9. Goldman DA, Klinger JD. Pseudomonas cepacia: biology, mechanisms of virulence, epidemiology. J Pediatr 1986; 108:806–812
     [Google Scholar]
  10. Parr TR, Moore RA, Moore LV, Hancock REW. Role of porins in intrinsic antibiotic resistance of Pseudomonas cepacia. Antimicrob Agents Chemother 1987; 31:121–123
     [Google Scholar]
  11. Thomassen MJ, Demko CA, Klinger JD, Stern RC. Pseudomonas cepacia colonization among patients with cystic fibrosis: a new opportunist. Am Rev Respir Dis 1985; 131:791–796
     [Google Scholar]
  12. Pedersen SS, Hoiby N, Shand GH, Pressler T. Antibody response to Pseudomonas aeruginosa antigens in cystic fibrosis. In: Hoiby N, Pedersen SS, Shand GH, Doring G, Holder IA. (eds) Pseudomonas aeruginosa infection. Antibiot Chemother 1989; 42:130–153
     [Google Scholar]
  13. Aronoff SC, Quinn FJ, Stern RC. Longitudinal serum IgG response to Pseudomonas cepacia surface antigens in cystic fibrosis. Pediatr Pulmonol 1991; 11:289–293
     [Google Scholar]
  14. Aronoff SC, Stern RC. Serum IgG antibody to outer membrane antigens of Pseudomonas cepacia and Pseudomonas aeruginosa in cystic fibrosis. J Infect Dis 1988; 157:934–940
     [Google Scholar]
  15. Hedit A, Monteil H, Richard C. O and H serotyping of Pseudomonas cepacia. J Clin Microbiol 1983; 18:738–740
     [Google Scholar]
  16. Stull TL, LiPuma JJ, Edlind TD. A broad spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis 1988; 157:280–286
     [Google Scholar]
  17. Galanos C, Luderitz O, Westphal O. A new method for the extraction of R-lipopolysaccharides. Eur J Biochem 1969; 9:245–249
     [Google Scholar]
  18. Qureshi N, Takayama K, Ribi E. Purification and structural determination of non-toxic lipid A obtained from the lipopolysaccharide of Salmonella typhimurium. J BiolChem 1982; 257:11808–11815
     [Google Scholar]
  19. Hancock IC, Poxton IR. Separation and purification of surface components. In: Bacterial cell surface techniques Chichester: John Wiley and Sons; 198891
     [Google Scholar]
  20. Hitchcock PJ, Brown TM. Morphological heterogeneity among salmonella lipopolysaccharide chemotypes in silver stained polyacrylamide gels. J Bacteriol 1983; 154:269–277
     [Google Scholar]
  21. Laemli UK. Cleavage of structural proteins during the assembly of the head protein of bacteriophage T4. Nature 1970; 227:680–685
     [Google Scholar]
  22. Tsai C-M, Frasch CE. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 1982; 119:115–119
     [Google Scholar]
  23. Towbin H, Staehlin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979; 76:4350–4354
     [Google Scholar]
  24. Scott BB, Barclay GR. Endotoxin-polymyxin complexes in an improved enzyme-immunosorbent assay for IgG antibodies in blood donor sera to gram-negative endotoxin core glycolipids. Vox Sang 1987; 52:272–280
     [Google Scholar]
  25. Wilkinson SG. Composition and structure of lipopoly saccharide from Pseudomonas aeruginosa. Rev Infect Dis 1983; 5:Suppl 5S941–S949
     [Google Scholar]
  26. McKevitt AI, Woods DE. Characterization of Pseudomonas cepacia isolates from patients with cystic fibrosis. J Clin Microbiol 1984; 19:291–293
     [Google Scholar]
  27. Nelson JW, Barclay GR, Govan JRW. Diagnosis of chronic Pseudomonas aeruginosa infection in cystic fibrosis by ELISA for anti-Pseudomonas LPS IgG antibodies. Serodiagn Immunother Infect Dis 1990; 4:9–16
     [Google Scholar]
  28. Editorial Pseudomonas cepacia—more than a harmless com mensal?. Lancet 1992:3391385–1386
     [Google Scholar]
  29. Palleroni NJ, Genus I. Pseudomonas. In: Kreig NR, Holt JC. (eds) Bergey’s Manual of systemic bacteriology, vol 1 Baltimore: The Williams and Wilkins Co.; 1984141
     [Google Scholar]
  30. Nelson JW, Barclay GR, Micklem LR, Poxton IR, Govan JRW. Production and characterisation of mouse monoclonal antibodies reactive with the lipopolysaccharide core of Pseudomonas aeruginosa. J Med Microbiol 1992; 36:358–365
     [Google Scholar]
  31. Neal DJ, Wilkinson SG. Lipopolysaccharides from Pseudo monas maltophilia: structural studies of the side-chain, core and lipid-A regions of the lipopolysaccharide from strain NCTC 10257. Eur J Biochem 1982; 128:143–149
     [Google Scholar]
  32. Fick RB, Naegel GP, Squier SU, Wood RE, Gee JBL, Reynolds HY. Proteins of the cystic fibrosis respiratory tract: fragmented immunoglobulin G opsonic antibody causing defective opsonophagocytosis. J Clin Invest 1984; 74:236–248
     [Google Scholar]
  33. Heck LW, Alarcon PG, Kulhauy RM, Morihara K, Russell MW, Mestecky JF. Degradation of IgA proteins by Pseudomonas aeruginosa elastase. J Immunol 1990; 144:2253–2257
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-39-1-39
Loading
Serum IgG and sputum IgA antibody to core lipopolysaccharide antigen from Pseudomonas cepacia in patients with cystic fibrosis
J. Med. Microbiol. 39, 39 (1993); https://doi.org/10.1099/00222615-39-1-39
/content/journal/jmm/10.1099/00222615-39-1-39
/content/journal/jmm/10.1099/00222615-39-1-39
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error