1887

Abstract

Summary

To assess the applicability of a whole-cell ELISA (WCE) with monoclonal antibodies (MAbs) for lipo-oligosaccharide (LOS) immunotyping of 675 meningococcal isolates obtained in 1989 and 1990 in the Netherlands and 57 isolates collected in 1974, of which the immunotype had been determined previously by microprecipitation, were analysed. Despite the lack of specific MAbs for L2 and L4, an algorithm was developed for the assignment of immunotypes on the basis of the reaction patterns of the reference strains and these isolates to a combination of 14 MAbs. The immunotypes found by WCE were in accordance with those obtained by microprecipitation and the results from WCE were reproducible. The distribution of immunotypes among isolates of the various serogroups in the Netherlands in 1989–1990 is presented. Based on the reaction patterns of the isolates, two main categories of related immunotypes could be distinguished among isolates of serogroups B and C: L2/L4 and L3/L1/L8. Some isolates of the latter category were of one immunotype, but many isolates expressed one or two additional immunotypes, either strongly or weakly, indicating that the differences in this category are quantitative rather than qualitative. The results of this study have demonstrated that the WCE method for LOS immunotyping is easily applicable and provides better definition of test strains for in-vitro bactericidal assays and research into pathogenesis.

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1994-10-01
2024-03-29
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References

  1. Frasch CE, Zollinger WD, Poolman JT. Serotype antigens of Neisseria meningitidis and a proposed scheme for designation of serotypes. Rev Infect Dis 1985; 7:504–510
    [Google Scholar]
  2. Tsai C-M, Mocca LF, Frasch CE. Immunotype epitopes of Neisseria meningitidis lipopolysaccharide types 1 through 8. Infect Immun 1987; 55:1652–1656
    [Google Scholar]
  3. Zollinger WD, Mandrell RE. Outer-membrane protein and lipopolysaccharide serotyping of Neisseria meningitidis by inhibition of a solid phase radioimmunoassay. Infect Immun 1977; 18:424–433
    [Google Scholar]
  4. Poolman JT, Hopman CTP, Zanen HC. Problems in the definition of meningococcal serotypes. FEMS Microbiol Lett 1982; 13:339–348
    [Google Scholar]
  5. Kim JJ, Mandrell RE, Zhen H, Westerink MAJ, Poolman JT, Griffiss JM. Electromorphic characterization and description of conserved epitopes of the lipooligosaccharides of group A Neisseria meningitidis. Infect Immun 1988; 56:2631–2638
    [Google Scholar]
  6. Crowe BA, Wall RA, Kusecek B et al. Clonal and variable properties of Neisseria meningitidis isolated from cases and carriers during and after an epidemic in the Gambia, West Africa. J Infect Dis 1989; 159:686–700
    [Google Scholar]
  7. Saukkonen K, Leinonen M, Abdillahi H, Poolman JT. Comparative evaluation of potential components for group B meningococcal vaccine by passive protection in the infant rat and in vitro bactericidal assay. Vaccine 1989; 7:325–328
    [Google Scholar]
  8. Zollinger WD, Mandrell RE. Type-specific antigens of group A Neisseria meningitidis; lipopolysaccharides and heat-modifiable outer membrane proteins. Infect Immun 1980; 28:451–458
    [Google Scholar]
  9. Poolman JT, Hopman CTP, Zanen HC. Colony variants of Neisseria meningitidis strain 2996 (B: 2b: P1.2): Influence of class-5 outer membrane proteins and lipopolysaccharides. J Med Microbiol 1985; 19:203–209
    [Google Scholar]
  10. Mandrell RE, Kim JJ, John CM et al. Endogenous sialylation of the lipooligosaccharides of Neisseria meningitidis. J Bacteriol 1991; 173:2823–2832
    [Google Scholar]
  11. Tsai C-M. Civin Cl. Eight lipooligosaccharides of Neisseria meningitidis react with a monoclonal antibody which binds lacto-N-neotetraose (Galβl -4GlcNacβl-3Galβl-4Glc). Infect Immun 1991; 59:3604–3609
    [Google Scholar]
  12. Mandrell RE, Zollinger WD. Lipopolysaccharide serotyping of Neisseria meningitidis by hemagglutination inhibition. infect Immun 1977; 16:471–475
    [Google Scholar]
  13. Jennings HJ, Johnson KG, Kenne L. The structure of an R-type oligosaccharide core obtained from some lipopolysaccharides of Neisseria meningitidis. Carbohydr Res 1983; 121:1983–241
    [Google Scholar]
  14. Jennings HJ, Beurret M, Gamian A, Michon F. Structure and immunochemistry of meningococcal lipooligosaccharides. Antonie Van Leeuwenhoek 1987; 53:519–522
    [Google Scholar]
  15. Dell A, Azadi P, Tiller P et al. Analysis of oligosaccharide epitopes of meningococcal lipopolysaccharides by fastatom-bombardment mass spectrometry. Carbohydr Res 1990; 200:59–76
    [Google Scholar]
  16. Difabio JL, Michon F, Brisson JR, Jennings HJ. Structures of the L1 and L6 core oligosaccharide epitopes of Neisseria meningitidis. Can J Chem 1990; 68:1029–1034
    [Google Scholar]
  17. Michon F, Beurrett M, Gamian A, Brisson JR, Jennings HJ. Structure of the L5 lipopolysaccharide core oligosaccharide of Neisseria meningitidis. J Biol Chem 1990; 256:1990–7247
    [Google Scholar]
  18. Schneider H, Griffiss JM, Boslego JW, Hitchcock PJ, Zahos KM, Apicella MA. Expression of paragloboside-like lipooligosaccharides may be a necessary component of gonococcal pathogenesis in men. J Exp Med 1991; 174:1601–1605
    [Google Scholar]
  19. Achtman M, Kusecek B, Morelli G et al. A comparison of the variable antigens expressed by clone IV-1 and subgroup III of Neisseria meningitidis serogroup A. J Infect Dis 1992; 165:1992–68
    [Google Scholar]
  20. Salih MAM, Danielsson D, Bäckman A, Caugant DA, Achtman M, Olcén P. Characterization of epidemic and nonepidemic Neisseria meningitidis serogroup A strains from Sudan and Sweden. J Clin Microbiol 1990; 28:1711–1719
    [Google Scholar]
  21. Poolman JT. Polysaccharides and membrane vaccines. Mizrahi A. Bacterial vaccines. Advances in Biotechnological Processes 13 New York: Wiley-Liss; 199057–86
    [Google Scholar]
  22. Slaterus KW. Serological typing of meningococci by means of micro-precipitation. Antonie Van Leeuwenhoek 1961; 27:305–315
    [Google Scholar]
  23. Abdillahi H, Poolman JT. Whole-cell ELISA for typing Neisseria meningitidis with monoclonal antibodies. FEMS Microbiol Lett 1987; 48:367–371
    [Google Scholar]
  24. Zollinger WD, Mandrell RE. Importance of complement source in bactericidal activity of human antibody and murine monoclonal antibody to meningococcal group B polysaccharide. Infect Immun 1983; 40:257–264
    [Google Scholar]
  25. Abdillahi H, Poolman JT. Neisseria meningitidis group B serosubtyping using monoclonal antibodies in whole-cell ELISA. Microb Pathog 1988; 4:27–32
    [Google Scholar]
  26. Verheul AFM, Braat AK, Leenhouts JM et al. Preparation, characterization, and immunogenicity of meningococcal immunotype L2 and L3,7,9 phosphoethanolamine group-containing oligosaccharide-protein conjugates. Infect Immun 1991; 59:1991–851
    [Google Scholar]
  27. Jones DM, Borrow R, Fox AJ, Gray S, Cartwright KA, Poolman JT. The lipooligosaccharide immunotype as a virulence determinant in Neisseria meningitidis. Microb Pathog 1992; 13:1992–224
    [Google Scholar]
  28. Scholten RJPM, Bijlmer HA, Poolman JT et al. Meningococcal disease in the Netherlands, 1958–1990: a steady increase in the incidence since 1982 partially caused by new serotypes and subtypes of Neisseria meningitidis. Clin Infect Dis 1993; 16:1993–246
    [Google Scholar]
  29. Poolman JT, Lind I, Jónsdóttir KE, Frøholm LO, Jones DM, Zanen HC. Meningococcal serotypes and serogroup B disease in north-west Europe. Lancet 1986; 2:555–558
    [Google Scholar]
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