1887

Journal of Medical Microbiology logo

Volume 43, Issue 5

Antibodies to meningococcal class 1 outer-membrane protein and its variable regions in patients with systemic meningococcal disease Free

Abstract

Summary

Antibodies to the meningococcal serosubtype-specific P1. 7, 16 protein and its variable regions (VR) were analysed in 28 convalescent sera drawn 8-36 months after systemic meningococcal disease by immunoblotting and enzyme immunoassay (EIA) methods. EIA antigens were the meningococcal P1. 7, 16 protein, produced in Bacillussubtilis, and peptides covering its VR1 (P1. 7 region) and VR2 (P1. 16 region) inserted into a bacterial penicillinase protein. In the immunoblotting method, three meningococcal reference strains were used; they expressed either the P1. 7, 16 protein, or only its VR1 or VR2 epitopes in their class 1 proteins. Both methods showed a strong IgG response in four sera to P1. 7, 16 and VR2, but not to VR1; 18 sera had no or weak anti-class 1 protein activity. The six remaining sera were positive only on blots. The VR2-specific sera had 30-fold higher bactericidal activity than those with negligible P1. 7, 16 responses. Previous vaccination of the patients with a B: 15: P1. 7, 16 meningococcal vaccine was associated with a strong anti-P1. 7, 16 and anti-VR2 booster response that declined with time. The subtype-specific antibody activity in some sera indicated colonisation after disease by meningococci with class 1 proteins different from the strain that had caused disease.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1995
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-43-5-335
1995-11-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jmm/43/5/medmicro-43-5-335.html?itemId=/content/journal/jmm/10.1099/00222615-43-5-335&mimeType=html&fmt=ahah

References

  1. Hitchcock P. Unified nomenclature for pathogenic Neisseria species. Clin Microbiol Rev 1989; 2:S64–S65
     [Google Scholar]
  2. Frasch C. E., Zollinger W. D., Poolman J. T. Serotype antigens of Neisseria meningitidis and a proposed scheme for designation of serotypes. Rev Infect Dis 1985; 7:504–510
     [Google Scholar]
  3. Barlow A. K., Heckels J. E., Clarke I. N. The class 1 outer membrane protein of Neisseria meningitidis: gene sequence and structural and immunological similarities to gonococcal porins. Mol Microbiol 1989; 3:131–139
     [Google Scholar]
  4. McGuinness B., Barlow A. K., Clark I. N. Deduced aminoacid sequences of class 1 protein (PorA) from three strains of Neisseria meningitidis. Synthetic peptides define the epitopes responsible for serosubtype specificity. J Exp Med 1990; 171:1871–1882
     [Google Scholar]
  5. Maiden M. C. J., Suker J., McKenna A. J., Bygraves J. A., Feavers I. M. Comparison of the class 1 outer membrane proteins of eight serological reference strains of Neisseria meningitidis. Mol Microbiol 1991; 5:727–736
     [Google Scholar]
  6. Feavers I. M., Heath A. B., Bygraves J. A., Maiden M. C. J. Role of horizontal genetic exchange in the antigenic variation of the class 1 outer membrane protein of Neisseria meningitidis. Mol Microbiol 1992; 6:489–495
     [Google Scholar]
  7. McGuinness B. T., Lambden P. R., Heckels J. E. Class 1 outer membrane protein of Neisseria meningitidis: epitope analysis of the antigenic diversity between strains, implications for subtype definition and molecular epidemiology. Mol Microbiol 1993; 7:505–514
     [Google Scholar]
  8. Van der Ley P., Heckels J. E., Virji M., Hoogerhout P., Poolman J. T. Topology of outer membrane porins in pathogenic Neisseria spp. Infect Immun 1991; 59:2963–2971
     [Google Scholar]
  9. Saunders N. B., Zollinger W. D., Rao V. B. A rapid and sensitive PCR strategy for amplification and sequencing of porA from a single colony-forming unit of Neisseria meningitidis. Gene 1993; 137:153–162
     [Google Scholar]
  10. Saukkonen K., Abdillahi H., Poolman J. T., Leinonen M. Protective efficacy of monoclonal antibodies to class 1 and class 3 outer membrane proteins of Neisseria meningitidis B: 15: P1.16 in infant rat infection model: new prospects for vaccine development. Microb Pathog 1987; 3:261–267
     [Google Scholar]
  11. Saukkonen K., Leinonen M., Abdillahi H., Poolman J. T. Comparative evaluation of potential components for group B meningococcal vaccine by passive protection in the infant rat and in vitro bactericidal assay. Vaccine 1989; 7:325–328
     [Google Scholar]
  12. Van der Ley P., Poolman J. T. Construction of a multivalent meningococcal vaccine strain based on the class 1 outer membrane protein. Infect Immun 1992; 60:3156–3161
     [Google Scholar]
  13. Van der Ley P., Van der Biezen J., Hohenstein P., Peeters C., Poolman J. T. Use of transformation to construct antigenic hybrids of the class 1 outer membrane protein in Neisseria meningitidis. Infect Immun 1993; 61:4217–4224
     [Google Scholar]
  14. Christodoulides M., McGuinness B. T., Heckels J. E. Immunization with synthetic peptides containing epitopes of the class 1 outer-membrane protein of Neisseria meningitidis: production of bactericidal antibodies on immunization with a cyclic peptide. J Gen Microbiol 1993; 139:1729–1738
     [Google Scholar]
  15. Wedege E., Frøholm L. O. Human antibody response to a group B serotype 2a meningococcal vaccine determined by immunoblotting. Infect Immun 1986; 51:571–578
     [Google Scholar]
  16. Milagres L. G., Ramos S. R., Sacchi C. T. Immune response of Brazilian children to a Neisseria meningitidis serogroup B outer membrane protein vaccine: comparison with efficacy. Infect Immun 1994; 62:4419–4424
     [Google Scholar]
  17. Wedege E., Bjune G., Frøholm L. O., Høiby E. A., Rosenqvist E. Immunoblotting studies of vaccinee and patient sera from a Norwegian serogroup B meningococcal vaccination trial. NIPH Ann 1991; 14:183–186
     [Google Scholar]
  18. Rosenqvist E., HØiby E. A., Wedege E. A new variant of serosubtype P1.16 in Neisseria meningitidis from Norway, associated with increased resistance to bactericidal antibodies induced by a serogroup B outer membrane protein vaccine. Microb Pathog 1993; 15:197–205
     [Google Scholar]
  19. Høiby E. A., Rosenqvist E., Frøholm L. O. Bactericidal antibodies after vaccination with the Norwegian meningococcal serogroup B outer membrane vesicle vaccine: a brief survey. NIPH Ann 1991; 14:147–156
     [Google Scholar]
  20. Bjune G., Høiby E. A., Grønnesby J. K. Effect of outer membrane vesicle vaccine against group B meningococcal disease in Norway. Lancet 1991; 338:1093–1096
     [Google Scholar]
  21. Nurminen M., Butcher S., Idänpään-Heikkilä I. The class 1 outer membrane protein of Neisseria meningitidis produced in Bacillus subtilis can give rise to protective immunity. Mol Microbiol 1992; 6:2499–2506
     [Google Scholar]
  22. Wedege E., Høiby E. A., Rosenqvist E., Frøholm L. O. Serotyping and subtyping of Neisseria meningitidis isolates by coagglutination, dot-blotting and ELISA. J Med Microbiol 1990; 31:195–201
     [Google Scholar]
  23. Garred P., Michaelsen T. E., Bjune G., Thiel A., Svejgaard A. A low serum concentration of mannan-binding protein is not associated with serogroup B or C meningococcal disease. Scand J Immunol 1993; 37:468–470
     [Google Scholar]
  24. Mandrell R. E., Zollinger W. D. Use of a zwitterionic detergent for the restoration of the antibody-binding capacity of electro-blotted meningococcal outer membrane proteins. J Immunol Methods 1984; 67:1–11
     [Google Scholar]
  25. Wedege E., Bryn K., Frøholm L. O. Restoration of antibody binding to blotted meningococcal outer membrane proteins using various detergents. J Immunol Methods 1988; 113:51–59
     [Google Scholar]
  26. HØiby E. A., Bjune G., Frøholm L. O. The Norwegian meningococcal serogroup B outer membrane vesicle vaccine protection trials: case tracing, meningococcal antigen detection and serological diagnosis. NIPH Ann 1991; 14:107–123
     [Google Scholar]
  27. Idänpään-Heikkilä I., Muttilainen S., WahlstrØm E. The antibody response to a prototype liposome vaccine containing Neisseria meningitidis outer membrane protein P1 produced in Bacillus subtilis. Vaccine 1995 (in press)
    [Google Scholar]
  28. Ambler R. P., Coulson A. F. W., Frère J. M. A standard numbering scheme for the class A β-lactamases. Biochem J 1991; 276:269–270
     [Google Scholar]
  29. Palva I., Sarvas M., Lehtovaara P., Sibakov M., Kääriäinen L. Secretion of Escherichia coli β-lactamase from Bacillus subtilis by the aid of α-amylase signal sequence. Proc Natl Acad Sci USA 1982; 79:5582–5586
     [Google Scholar]
  30. Mocca L. F., Frasch C. E. Sodium dodecyl sulfate-polyacrylamide gel typing system for characterization of Neisseria meningitidis isolates. J Clin Microbiol 1982; 16:240–244
     [Google Scholar]
  31. Rosenqvist E., HØiby E. A., Wedege E., Kusceck B., Achtman M. The 5C protein of Neisseria meningitidis is highly immunogenic in humans and induces bactericidal antibodies. J Infect Dis 1993; 167:1065–1073
     [Google Scholar]
  32. Wedege E., Kolberg J., Delvig A. Emergence of a new virulent clone within the ET-5 complex of serogroup B meningococci in Norway. Clin Diagn Lab Immunol 1995; 2:317–321
     [Google Scholar]
  33. Wedege E., Dalseg R., Caugant D. A., Poolman J. T., Frøholm L. O. Expression of an inaccessible P1.7 subtype epitope on meningococcal class 1 proteins. J Med Microbiol 1993; 38:23–28
     [Google Scholar]
  34. Guttormsen H.-K., Wetzler L. M., Solberg C. O. Humoral immune response to class 1 outer membrane protein during the course of meningococcal disease. Infect Immun 1994; 62:1437–1443
     [Google Scholar]
  35. Zollinger W. D., Mandrell R. E. Studies of the human antibody response to specific meningococcal outer membrane proteins of serotypes 2 and 15. Med Trop 1983; 43:143–147
     [Google Scholar]
  36. Maeland J. A., Wedege E. Serum antibodies to cross-reactive Neisseria outer membrane antigens in healthy persons and patients with meningococcal disease. APMIS 1989; 97:774–780
     [Google Scholar]
  37. Zollinger W. D., Boslego J. W., Moran E. E., Brandt B. L. Effect of vaccination with meningococcal outer membrane protein vaccine on subsequent antibody response to carriage and natural infection. In Conde-Glez C. J., Morse S., Rice P. (eds) Pathobiology and immunology of Neisseriaceae Instituto Nacional de Salud Publica; 1994954–960
     [Google Scholar]
  38. Poolman J. T., Hopman C. T. P., Zanen H. C. Immunogenicity of meningococcal antigens as detected in patient sera. Infect Immun 1983; 40:398–406
     [Google Scholar]
  39. Mandrell R. E., Zollinger W. D. Human immune response to meningococcal outer membrane protein epitopes after natural infection or vaccination. Infect Immun 1989; 57:1590–1598
     [Google Scholar]
  40. Orren A., Warren R. E., Potter P. C., Jones A. M., Lachmann P. J., Poolman J. T. Antibodies to meningococcal class 1 outer membrane proteins in South African complement-deficient and complement-sufficient subjects. Infect Immun 1992; 60:4510–4516
     [Google Scholar]
  41. Peeters C. C. A. M., Schuller M., Westdijk J., Poolman J. T. Specificity of bactericidal antibody responses in volunteers immunized with meningococcal B: 15: P1.7, 16 outer membrane proteins complexed to C polysaccharide and in patients infected with B: 15: P1.7, 16. In Conde-Glez C. J., Morse S., Rice P. (eds) Pathobiology and immunology of Neisseriaceae Instituto Nacional de Salud Publica; 1994880–887
     [Google Scholar]
  42. Caugant D. A., Høiby E. A., Magnus P. Asymptomatic carriage of Neisseria meningitidis in a randomly sampled population. J Clin Microbiol 1994; 32:323–330
     [Google Scholar]
  43. Rosenqvist E., Bjune G., Feiring B. Changes in carrier status of Neisseria meningitidis in teenagers during a group B outer membrane vaccination trial in Norway. In Conde-Glez C. J., Morse S., Rice P. (eds) Pathobiology and immunology of Neisseriaceae Instituto Nacional de Salud Publica; 1994895–901
     [Google Scholar]
  44. Zollinger W. D., Moran E. Meningococcal vaccines—present and future. Trans R Soc Trop Med Hyg 1991; 85: Suppl 137–43
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-43-5-335
Loading
Antibodies to meningococcal class 1 outer-membrane protein and its variable regions in patients with systemic meningococcal disease
J. Med. Microbiol. 43, 335 (1995); https://doi.org/10.1099/00222615-43-5-335
/content/journal/jmm/10.1099/00222615-43-5-335
/content/journal/jmm/10.1099/00222615-43-5-335
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error