1887

Abstract

There is great interest in the use of antimicrobial agents for the prevention and treatment of plaque-related oral diseases and many publications have reported the results of studies in which the minimum inhibitory concentrations of agents for cariogenic and periodontopathogenic bacteria have been determined. However, such data are relevant only to situations where the organisms of interest are in aqueous suspension, whereas in caries and the inflammatory periodontal diseases the target organisms are in the form of biofilms. On the basis of studies with medically important bacteria, it has been established that bacteria in biofilms are invariably less susceptible to antimicrobial agents than their planktonic counterparts. Therefore, in the laboratory assessment of agents which may be suitable for treating plaque-related diseases, the target organisms should be in the form of biofilms. While laboratory evaluation of chemical agents for the prevention of plaque formation has usually employed biofilm-based models, the search for antimicrobial agents effective in the treatment of plaque-related diseases has not. Therefore, there are few data available regarding those characteristics of antimicrobial agents (e.g., their biofilm eliminating concentrations or biofilm killing concentrations) that could be used to judge their suitability for treating plaque-related diseases. In this review the limited information available concerning the antimicrobial susceptibility of oral bacteria in biofilms is presented.

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1996-02-01
2024-04-20
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References

  1. McCabe R. M., Keyes P. H., Howell A. An in vitro method for assessing the plaque forming ability of oral bacteria. Arch Oral Biol 1967; 12:1653–1656
    [Google Scholar]
  2. Rundegren J., Simonsson T., Petersson L., Hansson E. Effect of delmopinol on the cohesion of glucan-containing plaque formed by Streptococcus mutans in a flow cell system. J Dent Res 1992; 71:1792–1796
    [Google Scholar]
  3. Herles S., Olsen S., Afflitto J., Gaffar A. Chemostat flow cell system: an in vitro model for the evaluation of antiplaque agents. J Dent Res 1994; 73:1748–1755
    [Google Scholar]
  4. Coulter W. A., Russell C. Effect of chlorhexidine on plaque development in an artificial mouth. Microbios 1976; 16:21–28
    [Google Scholar]
  5. Nichols W. W. Susceptibility of biofilms to toxic compounds. In Characklis W. G., Wilderer P. A. (eds) Structure and function of biofilms Chichester: John Wiley and Sons; 1989321–331
    [Google Scholar]
  6. Anwar H., Dasgupta M. K., Costerton J. W. Testing the susceptibility of bacteria in biofilms to antibacterial agents. Antimicrob Agents Chemother 1990; 34:2043–2046
    [Google Scholar]
  7. Costerton J. W., Cheng N. J., Geesey G. G Bacterial biofilms in nature and disease. Annu Rev Microbiol 1987; 41:435–464
    [Google Scholar]
  8. Wilcox M. H. Treating medical device associated infections. In Wimpenny J., Nichols W., Stickler D., Lappin-Scott H. (eds) Bacterial biofilms and their control in medicine and industry CardiF: Bioline; 1994155–158
    [Google Scholar]
  9. Elliott T. S. J. Intravascular-device infections. J Med Microbiol 1988; 27:161–167
    [Google Scholar]
  10. Dickinson G. M., Bisno A. L. Infections associated with indwelling devices: infections related to extravascular devices. Antimicrob Agents Chemother 1989; 33:602–607
    [Google Scholar]
  11. Lambert P. A., Giwercman B., Hoiby N. Chemotherapy of Pseudomonas infections in cystic fibrosis. In Wimpenny J., Nichols W., Stickler D., Lappin-Scott H. (eds) Bacterial biofilms and their control in medicine and industry Cardiff: Bioline; 1994151–153
    [Google Scholar]
  12. Anwar H., Costerton J. W. Enhanced activity of combinations of tobramycin and piperacillin for eradication of sessile biofilm cells of Pseudomonas aeruginosa . Antimicrob Agents Chemother 1990; 34:1666–1671
    [Google Scholar]
  13. Costerton J. W., Khoury A. E., Ward K. H., Anwar H. Practical measures to control device-related bacterial infections. Int J Artif Organs 1993; 16:765–770
    [Google Scholar]
  14. Nichols W. W. Biofilm permeability to antimicrobial agents. In Wimpenny J., Nichols W., Stickler D., Lappin-Scott H. (eds) Bacterial biofilms and their control in medicine and industry Cardiff: Bioline; 1994141–149
    [Google Scholar]
  15. Kurian S., Lorian V. Discrepancies between results obtained by agar and broth techniques in testing of drug combinations. J Clin Microbiol 198011527–529
    [Google Scholar]
  16. Al-Hiti M. M. A., Gilbert P. A note on inoculum reproducibility: solid culture versus liquid culture. J Appl Bacteriol 1983; 55:173–176
    [Google Scholar]
  17. Dobson J., Wilson M. Sensitisation of oral bacteria in biofilms to killing by light from a low-power laser. Arch Oral Biol 1992; 37:883–887
    [Google Scholar]
  18. Bums T., Wilson M., Pearson G. Sensitisation of cariogenic bacteria in biofilms to killing by low power laser light. Proceedings of the 4th International Conference on Lasers Dentistry in 199466
    [Google Scholar]
  19. Caufield P. W., Allen D. N, Childers N. K. In vitro susceptibilities of suspected periodontopathogenic anaerobes as determined by membrane transfer assay. Antimicrob Agents Chemother 1987; 31:1989–1993
    [Google Scholar]
  20. Millward T. A., Wilson M. The effect of chlorhexidine on Streptococcus sanguis biofilms. Microbios 1989; 58:155–164
    [Google Scholar]
  21. Nichols W. W., Evans M. J., Slack M. P. E., Walmsley H. L. The penetration of antibiotics into aggregates of mucoid and nonmucoid Pseudomonas aeruginosa . J Gen Microbiol 1989; 135:1291–1303
    [Google Scholar]
  22. Stewart P. S., Griebe T., Srinivasan R. Comparison of respiratory activity and culturability during monochloramine disinfection of binary population biofilms. Appl Environ Microbiol 1994; 60:1690–1692
    [Google Scholar]
  23. Bradshaw D. J., McKee A. S., Marsh P. D. Prevention of population shifts in oral microbial communities in vitro by low fluoride concentrations. J Dent Res 1990; 69:436–441
    [Google Scholar]
  24. Marsh P. D., Bradshaw D. J. Microbiological effects of new agents in dentifrices for plaque control. Int Dent J 1993; 43:399–406
    [Google Scholar]
  25. Nichols W. W. Sensitivity of bacteria in biofilms to antibacterial agents. In Denyer S.P., Gorman S.P., Sussman M. (eds) Microbial biofilms: formation and control Society for Applied Bacteriology Technical Series no. 30 Oxford: Blackwell Scientific Publications Ltd; 1993187–200
    [Google Scholar]
  26. Brown M. R. W., Gilbert P. Sensitivity of biofilms to antimicrobial agents. In Quesnel L. B., Gilbert P., Handley P. S. (eds) Microbial cell envelopes: interactions and biofilms Oxford: Blackwell Scientific Publications; 199387–97
    [Google Scholar]
  27. Lappin-Scott H. M., Jass J., Costerton J. W. Microbial biofilm formation and characterization. In Denyer S. P., Gorman S. P., Sussman M. (eds) Microbial biofilms: formation and control Society for Applied Bacteriology Technical Series no. 30 Oxford: Blackwell Scientific Publications Ltd; 19931–12
    [Google Scholar]
  28. Wimpenny J. W. T., Peters A., Scourfield M. A. Modeling spatial gradients. In Characklis W. G., Wilderer P. A. (eds) Structure and function of biofilms Chichester: John Wiley and Sons; 1989111–127
    [Google Scholar]
  29. Wilson M., Kpendema H., Noar J. H., Hunt N., Mordan N. J. Corrosion of intra-oral magnets in the presence and absence of biofilms of Streptococcus sanguis . Biomaterials 1995; 16:721–725
    [Google Scholar]
  30. Wilson M., Patel H., Fletcher J. Susceptibility of biofilms of Streptococcus sanguis to chlorhexidine gluconate and cetylpyridinium chloride. Oral Microbiol Immunol 1995 (in press)
    [Google Scholar]
  31. Wilson M. Lethal photosensitisation of Streptococcus sanguis biofilms. In Wimpenny J., Handley P., Gilbert P., Lappin-Scott H. (eds) Life and death in microbial biofilm Cardiff: Bioline; 1995143–147
    [Google Scholar]
  32. Le Magrex E., Jacquelin L. F., Carquin J., Brisset L., Choisy C. Antiseptic activity of some antidental plaque chemicals on Streptococcus mutans biofilms. Pathol Biol 1993; 41:364–368
    [Google Scholar]
  33. Rogers J., Lee J. V., Dennis P. J., Keevil C. W. Continuous culture biofilm model for the survival and growth of Legionella pneumophila and associated protozoa in potable water systems. In Morris R., Alexander P., Wynn-Jones P., Sellwood J. (eds) Health-related water microbiology London: IAWPRC; 1991192–200
    [Google Scholar]
  34. Caldwell D. E., Korber D. R., Lawrence J. R. Confocal laser microscopy and computer image analysis in microbial ecology. Adv Microb Ecol 1992; 12:1–68
    [Google Scholar]
  35. Caldwell D. E., Korber D. R., Lawrence J. R. Analysis of biofilm formation using 2D vs 3D digital imaging. J Appl Bacteriol 1993; 74:52S–66S
    [Google Scholar]
  36. Kinniment S. L. Cryosectioning of biofilm. In Wimpenny J., Nichols W., Stickler D., Lappin-Scott H. (eds) Bacterial biofilms and their control in medicine and industry Cardiff: Bioline; 199453–56
    [Google Scholar]
  37. Characklis W. G., Cooksey K. E. Biofilms and microbial fouling. Adv Appl Microbiol 1983; 29:93–138
    [Google Scholar]
  38. Hoyle B. D., Jass J., Costerton J. W. The biofilm glycocalyx as a resistance factor. J Antimicrob Chemother 1990; 26:1–5
    [Google Scholar]
  39. Nichols W. W. Biofilms, antibiotics and penetration. Rev Med Microbiol 1991; 2:177–181
    [Google Scholar]
  40. Nichols W. W., Dorrington S. M., Slack M. P. E., Walmsley H. L. Inhibition of tobramycin diffusion by binding to alginate. Antimicrob Agents Chemother 1988; 32:518–523
    [Google Scholar]
  41. Brown M. R. W., Allison D. G., Gilbert P. Resistance of bacterial biofilms to antibiotics: a growth-rate related effect?. J Antimicrob Chemother 1988; 22:777–780
    [Google Scholar]
  42. Evans R. C., Holmes C. J. Effect of vancomycin hydrochloride on Staphylococcus epidermidis biofilm associated with silicone elastomer. Antimicrob Agents Chemother 1987; 31:889–894
    [Google Scholar]
  43. Prosser B. L. T., Taylor D., Dix B. A., Cleeland R. Method of evaluating effects of antibiotics upon bacterial biofilm. Antimicrob Agents Chemother 1987; 31:1502–1506
    [Google Scholar]
  44. Brown M. R. W. Nutrient depletion and antibiotic susceptibility. J Antimicrob Chemother 1977; 3:198–201
    [Google Scholar]
  45. Brown M. R. W., Williams P. Influence of substrate limitation and growth phase on sensitivity to antimicrobial agents. J Antimicrob Chemother 1985; 15: Suppl A 7–14
    [Google Scholar]
  46. Ellwood D. C., Tempest D. W. Effects of environment on bacterial cell wall content and composition. Adv Microb Physiol 1972; 7:83–117
    [Google Scholar]
  47. Brown M. R. W., Williams P. The influence of environment on envelope properties affecting survival of bacteria in infections. Annu Rev Microbiol 1985; 39:527–556
    [Google Scholar]
  48. Costerton J. W. Structure and plasticity at various organization levels in the bacterial cell. Can J Microbiol 1988; 34:513–521
    [Google Scholar]
  49. Davies D. G., Chakrabarty A. M., Geesey G.G. Exopolysaccharide production in biofilms: substratum activation of alginate gene expression by Pseudomonas aeruginosa . Appl Environ Microbiol 1993; 59:1181–1186
    [Google Scholar]
  50. Williams P., Stewart G. S. A. B. Cell density dependent control of gene expression in bacteria—implications for biofilm development and control. In Wimpenny J., Nichols W., Stickler D., Lappin-Scott H. (eds) Bacterial biofilms and their control in medicine and industry Cardiff: Bioline; 19949–12
    [Google Scholar]
  51. Donoghue H. D., Perrons C. J. Establishment of defined mixed bacterial plaques on teeth in a laboratory microcosm (model mouth). Microb Ecol Health Dis 1988; 1:193–200
    [Google Scholar]
  52. Gilbert P., Allison D. G., Evans D. J., Handley P. S., Brown M. R. W. Growth rate control of adherent bacterial populations. Appl Environ Microbiol 1989; 55:1308–1311
    [Google Scholar]
  53. Wilson M. Laboratory assessment of antimicrobial agents for the treatment of chronic periodontitis. Microb Ecol Health Dis 1993; 6:143–145
    [Google Scholar]
  54. ten Cate J. M., Marsh P. D. Procedures for establishing efficacy of antimicrobial agents for chemotherapeutic caries prevention. J Dent Res 1994; 73:695–703
    [Google Scholar]
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