1887

Abstract

Examination of strain differences in the susceptibility of mice to experimental respiratory tract infection with penicillin-resistant TUM19 revealed that a fatal infection model could be induced in immunocompetent CBA/J mice, but not in C3H/HeN, C57BL/6 or ICR mice. After intranasal instillation of . 10 cfu of , the bacterial counts in the lungs of CBA/J mice increased from 10 to 10 cfu after 3-5 days, and gradually increased thereafter. The challenge organisms localised mainly in the lungs until 14 days after infection. Mice began to die . 7 days after infection, and by 3 weeks most of the mice had died. Histopathologically, infiltration of neutrophils and lymphocytes around bronchi was observed from 1 day after infection, and fibrin deposition was seen in alveolar and bronchial spaces from 5 days. This model may be useful for investigating therapy of respiratory tract infection caused by penicillin-resistant because its pathological features resemble those observed in the human disease.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-45-5-319
1996-11-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jmm/45/5/medmicro-45-5-319.html?itemId=/content/journal/jmm/10.1099/00222615-45-5-319&mimeType=html&fmt=ahah

References

  1. Woodhead M. A., Macfarlane J. T., McCracken J. S., Rose D. H., Finch R. G. Prospective study of the aetiology and outcome of pneumonia in the community. Lancet 1987; 1:671–674
    [Google Scholar]
  2. Pachon J., Prados M. D., Capote F., Cuello J. A., Gamacho J., Verano A. Severe community-acquired pneumonia. Etiology, prognosis, and treatment. Am Rev Respir Dis 1990; 142:369–373
    [Google Scholar]
  3. Appelbaum P. C. Antimicrobial resistance in Streptococcus pneumoniae: an overview. Clin Infect Dis 1992; 15:77–83
    [Google Scholar]
  4. Baquero F., Martinez-Beltrán J., Loza E. A review of antibiotic resistance patterns of Streptococcus pneumoniae in Europe. J Antimicrob Chemother 1991; 28: Suppl C 31–38
    [Google Scholar]
  5. Liñares J., Alonso T., Pérez J. L. Decreased susceptibility of penicillin-resistant pneumococci to twenty-four β-lactam antibiotics. J Antimicrob Chemother 1992; 30:279–288
    [Google Scholar]
  6. Azoulay-Dupuis E., Vallée E., Veber B., Bédos J.P., Bauchet J., Pocidalo J.-J. In vivo efficacy of a new fluoroquinolone, sparfloxacin, against penicillin-susceptible and -resistant and multiresistant strains of Streptococcus pneumoniae in a mouse model of pneumonia. Antimicrob Agents Chemother 1992; 36:2698–2703
    [Google Scholar]
  7. Moine P., Vallée E., Azoulay-Dupuis E. In vivo efficacy of a broad-spectrum cephalosporin, ceftriaxone, against penicillin-susceptible and -resistant strains of Streptococcus pneumoniae in a mouse pneumonia model. Antimicrob Agents Chemother 1994; 38:1953–1958
    [Google Scholar]
  8. Boulnois G. J. Pneumococcal proteins and the pathogenesis of disease caused by Streptococcus pneumoniae. J Gen Microbiol 1992; 138:249–259
    [Google Scholar]
  9. Briles D. E., Horowitz J., McDaniel L. S. Genetic control of the susceptibility to pneumococcal infection. Curr Top Microbiol Immunol 1986; 124:103–120
    [Google Scholar]
  10. Japanese Society for Chemotherapy Determination of minimum inhibitory concentration (MIC) by the micro-dilution method. Chemotherapy 1990; 38:102–105
    [Google Scholar]
  11. Briles D. E., Crain M. J., Gray B. M., Forman C., Yother J. Strong association between capsular type and virulence for mice among human isolates of Streptococcus pneumoniae. Infect Immun 1992; 60:111–116
    [Google Scholar]
  12. Johnston R. B. Pathogenesis of pneumococcal pneumonia. Rev Infect Dis 1991; 13: Suppl 6S509–S517
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-45-5-319
Loading
/content/journal/jmm/10.1099/00222615-45-5-319
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error