1887

Abstract

Within the community, 10 % of acquired pneumonia is caused by . -acetyl-cysteine (NAC) is one of the most commonly used mucolytics in respiratory diseases, but its effect on infection has not yet been investigated. In this study, our aim was to investigate whether NAC influences the replication of After determining that NAC does have an effect on replication, the effect of an alternative drug called Ambroxol (Ax) was investigated.

The effect of NAC and Ax was studied on infected A549 and McCoy cells. Furthermore, the influence of NAC and Ax was examined in mice infected intranasally with .

NAC treatment resulted in approximately sixfold more efficient growth in tissue culture compared to the untreated control cells, and this effect was shown to be based on the increased binding of the bacterium to the host cells. The infected mice to which NAC was given had prolonged and more severe infections than the control mice. Ax decreased replication , which was partially associated with the increased expression of indolamine 2,3-dioxygenase. In animals, using the adapted usual human dose, Ax did not alter the number of recoverable .

Based on our results, it might be recommended that a mucolytic agent other than NAC, such as Ax, be used in respiratory diseases suspected to be caused by .

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2018-05-01
2024-03-29
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References

  1. Mokhtari V, Afsharian P, Shahhoseini M, Kalantar SM, Moini A. A review on various uses of N-acetyl cysteine. Cell J 2017; 19:11–17[PubMed]
    [Google Scholar]
  2. Beeh KM, Beier J, Esperester A, Paul LD. Antiinflammatory properties of ambroxol. Eur J Med Res 2008; 13:557–562[PubMed]
    [Google Scholar]
  3. Stetinová V, Herout V, Kvetina J. In vitro and in vivo antioxidant activity of ambroxol. Clin Exp Med 2004; 4:152–158 [View Article][PubMed]
    [Google Scholar]
  4. Choroszy-Król I, Frej-Mądrzak M, Hober M, Sarowska J, Jama-Kmiecik A. Infections caused by Chlamydophila pneumoniae. Adv Clin Exp Med Off Organ Wroclaw Med Univ 2014; 23:123–126 [View Article]
    [Google Scholar]
  5. Abromaitis S, Stephens RS. Attachment and entry of Chlamydia have distinct requirements for host protein disulfide isomerase. PLoS Pathog 2009; 5:e1000357 [View Article][PubMed]
    [Google Scholar]
  6. Moelleken K, Hegemann JH. The Chlamydia outer membrane protein OmcB is required for adhesion and exhibits biovar-specific differences in glycosaminoglycan binding. Mol Microbiol 2008; 67:403–419 [View Article][PubMed]
    [Google Scholar]
  7. Lazarev VN, Borisenko GG, Shkarupeta MM, Demina IA, Serebryakova MV et al. The role of intracellular glutathione in the progression of Chlamydia trachomatis infection. Free Radic Biol Med 2010; 49:1947–1955 [View Article][PubMed]
    [Google Scholar]
  8. Burián K, Hegyesi H, Buzás E, Endrész V, Kis Z et al. Chlamydophila (Chlamydia) pneumoniae induces histidine decarboxylase production in the mouse lung. Immunol Lett 2003; 89:229–236 [View Article][PubMed]
    [Google Scholar]
  9. Caldwell HD, Kromhout J, Schachter J. Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis. Infect Immun 1981; 31:1161–1176[PubMed]
    [Google Scholar]
  10. Yuan JS, Reed A, Chen F, Stewart CN. Statistical analysis of real-time PCR data. BMC Bioinformatics 2006; 7:85 [View Article][PubMed]
    [Google Scholar]
  11. Tattersall AB, Bridgman KM, Huitson A. Acetylcysteine (Fabrol) in chronic bronchitis–a study in general practice. J Int Med Res 1983; 11:279–284 [View Article][PubMed]
    [Google Scholar]
  12. de Flora S, Grassi C, Carati L. Attenuation of influenza-like symptomatology and improvement of cell-mediated immunity with long-term N-acetylcysteine treatment. Eur Respir J 1997; 10:1535–1541 [View Article][PubMed]
    [Google Scholar]
  13. Aslam S, Trautner BW, Ramanathan V, Darouiche RO. Combination of tigecycline and N-acetylcysteine reduces biofilm-embedded bacteria on vascular catheters. Antimicrob Agents Chemother 2007; 51:1556–1558 [View Article][PubMed]
    [Google Scholar]
  14. Zhao T, Liu Y. N-acetylcysteine inhibit biofilms produced by Pseudomonas aeruginosa. BMC Microbiol 2010; 10:140 [View Article][PubMed]
    [Google Scholar]
  15. Olofsson AC, Hermansson M, Elwing H. N-acetyl-L-cysteine affects growth, extracellular polysaccharide production, and bacterial biofilm formation on solid surfaces. Appl Environ Microbiol 2003; 69:4814–4822 [View Article][PubMed]
    [Google Scholar]
  16. Allen M, Bailey C, Cahatol I, Dodge L, Yim J et al. Mechanisms of control of Mycobacterium tuberculosis by NK cells: role of glutathione. Front Immunol 2015; 6:508 [View Article][PubMed]
    [Google Scholar]
  17. Haggerty CL, Gottlieb SL, Taylor BD, Low N, Xu F et al. Risk of sequelae after Chlamydia trachomatis genital infection in women. J Infect Dis 2010; 201:134–155 [View Article][PubMed]
    [Google Scholar]
  18. Yang B, Yao DF, Ohuchi M, Ide M, Yano M et al. Ambroxol suppresses influenza-virus proliferation in the mouse airway by increasing antiviral factor levels. Eur Respir J 2002; 19:952–958 [View Article][PubMed]
    [Google Scholar]
  19. Morice A, Kardos P. Comprehensive evidence-based review on European antitussives. BMJ Open Respir Res 2016; 3:e000137 [View Article][PubMed]
    [Google Scholar]
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