1887

Abstract

The molecular mechanism of resistance to tetracycline involves mutations in the primary binding site of the ribosome. A resistance or reduced susceptibility to tetracycline could be the result of single, double or triple mutations in the 16S gene of . We investigated if the genotype was correlated to tetracycline resistance as determined phenotypically for 96 . isolates in the gastroesophageal mucosa of Venezuelan individual hosts. E-test for antimicrobial susceptibility test and real-time PCR for the detection of 16S gene mutations were performed in 96 . isolates (48 obtained from antrum, and 48 from oesophagus) from eight dyspeptic patients. In the gastric mucosa, 38 isolates were identified sensitive and 10 resistant to tetracycline by E-test, whereas 44 sensitive and 4 resistant isolates were found in the oesophagus. Real-time PCR detection of the 16S gene exhibited mutants with a single base-pair substitution (AGA GA) in six antrum isolates and seven oesophagus isolates, whereas only three harboured a low level of tetracycline resistance . Our results indicate that real-time PCR detection of 16S is a reliable method to classify among tetracycline-resistant genotypes and useful in patients who have experienced a first-line treatment failure with triple therapy.

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2019-09-01
2024-03-29
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