1887

Abstract

The objective of this study was to determine the distribution of staphylococcal cassette chromosome (SCC) elements in meticillin-resistant coagulase-negative staphylococci (MR-CoNS) isolated from a tertiary-care hospital in Mexico and to examine the relationship to drug resistance. Fifty selected MR-CoNS isolates collected from catheters (=15), blood (=15), bone (=9), bronchial lavage (=2) and urine (=2) and one isolate each from an abscess, cerebrospinal fluid, eye, pleural effusion, synovial fluid, tracheal aspirate and wound secretion were examined. Susceptibility testing was performed by the broth microdilution method. SCC types were determined by multiplex PCR and PFGE was carried out as described previously for . Among the MR-CoNS strains studied, the most frequently isolated species were (=26) and (=13). (=5), (=3), (=1), (=1) and the recently described species (=1) were also identified. The most frequent MR-CoNS genotype identified was SCC type IVa in isolates, which also showed a high diversity in their PFGE patterns. A clone was found that amplified both SCC III and V elements in five isolates examined. The single MR isolate harboured SCC type IVd and the single MR isolate harboured SCC type I. The carriage of SCC type III was associated with resistance or intermediate resistance to meropenem ( <0.05). These results confirm the high prevalence of SCC IVa and the high genetic diversity among MR-CoNS strains. As far as is known, this is the first report describing the newly identified possessing SCC IVd and harbouring SCC type I. MR-CoNS harbouring SCC type III were found to be more resistant to meropenem.

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2010-03-01
2024-03-28
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