1887

Journal of Medical Microbiology logo

Volume 60, Issue 12

Vitamin E improves the efficacy of tigecycline and daptomycin in an animal model of wounds infected with meticillin-resistant Free

Abstract

A relevant bacterial load in cutaneous wounds significantly interferes with the normal process of healing. Vitamin E (VE) is a known immunomodulator and immune enhancer. Here, it was shown that administration of VE before infection was effective at increasing the antimicrobial activity of daptomycin (DAP) or tigecycline (TIG) in a mouse model of wound infection caused by meticillin-resistant (MRSA). A wound was established through the panniculus carnosus of mice and inoculated with MRSA. Mice were assigned to six groups: a VE pre-treated group with no antibiotics given after MRSA challenge; two VE pre-treated groups with DAP or TIG given after MRSA challenge; two groups treated with DAP or TIG only after MRSA challenge; and a control group that did not receive any treatment. Mice receiving each antibiotic alone showed a 3 log decrease in the number of c.f.u. recovered compared with the control group, mice treated with VE plus TIG had a 4 log decrease, whilst mice treated with VE plus DAP had the largest decrease in c.f.u. recovered (5 logs). The increased antimicrobial effect seen from treatment with VE plus antibiotics was associated with increased levels of natural killer cell cytotoxicity, with a more pronounced increase in leukocyte populations in mice treated with VE plus DAP. These data suggest that treatment with VE prior to infection and subsequent antibiotic treatment act in synergy.

  • Received:
  • Accepted:
  • Published Online:
© 2011 SGM
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.032516-0
2011-12-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/60/12/1806.html?itemId=/content/journal/jmm/10.1099/jmm.0.032516-0&mimeType=html&fmt=ahah

References

  1. Arbeit R. D., Maki D., Tally F. P., Campanaro E., Eisenstein B. I. Daptomycin 98-01 and 99-01 Investigators 2004; The safety and efficacy of daptomycin for the treatment of complicated skin and skin-structure infections. Clin Infect Dis 38:1673–1681 [View Article][PubMed]
     [Google Scholar]
  2. Azzi A., Gysin R., Kempná P., Ricciarelli R., Villacorta L., Visarius T., Zingg J.-M. 2003; The role of α-tocopherol in preventing disease: from epidemiology to molecular events. Mol Aspects Med 24:325–336 [View Article][PubMed]
     [Google Scholar]
  3. Babizhayev M. A. 2010; New concept in nutrition for the maintenance of the aging eye redox regulation and therapeutic treatment of cataract disease; synergism of natural antioxidant imidazole-containing amino acid-based compounds, chaperone, and glutathione boosting agents: a systemic perspective on aging and longevity emerged from studies in humans. Am J Ther 17:373–389[PubMed]
     [Google Scholar]
  4. Bouza E. 2009; New therapeutic choices for infections caused by methicillin-resistant Staphylococcus aureus . Clin Microbiol Infect 15:Suppl. 744–52 [View Article][PubMed]
     [Google Scholar]
  5. Breen J. O. 2010; Skin and soft tissue infections in immunocompetent patients. Am Fam Physician 81:893–899[PubMed]
     [Google Scholar]
  6. Brigelius-Flohé R., Kelly F. J., Salonen J. T., Neuzil J., Zingg J. M., Azzi A. 2002; The European perspective on vitamin E: current knowledge and future research. Am J Clin Nutr 76:703–716[PubMed]
     [Google Scholar]
  7. CLSI 2003; Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard. M7-A6. Wayne, PA: Clinical and Laboratory Standards Institute;
  8. Coutinho H. D., Costa J. G., Lima E. O., Falcão-Silva V. S., Siqueira-Júnior J. P. 2009; Potentiating effect of Mentha arvensis and chlorpromazine in the resistance to aminoglycosides of methicillin-resistant Staphylococcus aureus . In Vivo 23:287–289[PubMed]
     [Google Scholar]
  9. De la Fuente M., Victor V. M. 2000; Anti-oxidants as modulators of immune function. Immunol Cell Biol 78:49–54 [View Article][PubMed]
     [Google Scholar]
  10. Elliott T. S., Lambert P. A. 1999; Antibacterial resistance in the intensive care unit: mechanisms and management. Br Med Bull 55:259–276 [View Article][PubMed]
     [Google Scholar]
  11. Elsner P. 2006; Antimicrobials and the skin physiological and pathological flora. Curr Probl Dermatol 33:35–41 [View Article][PubMed]
     [Google Scholar]
  12. Fang C. H., Peck M. D., Alexander J. W., Babcock G. F., Warden G. D. 1990; The effect of free radical scavengers on outcome after infection in burned mice. J Trauma 30:453–456[PubMed] [CrossRef]
     [Google Scholar]
  13. Finch J. M., Turner R. J. 1996; Effects of selenium and vitamin E on the immune responses of domestic animals. Res Vet Sci 60:97–106 [View Article][PubMed]
     [Google Scholar]
  14. Fitzgerald P. A., Lopez C. 1986; Natural killer cells active against viral, bacterial, protozoan, and fungal infections. In Immunobiology of Natural Killer Cells pp. 107–132 Edited by Lotzovà E., Herberman R. B. Boca Raton: CRC Press;
     [Google Scholar]
  15. Han S. N., Wu D., Ha W. K., Beharka A., Smith D. E., Bender B. S., Meydani S. N. 2000; Vitamin E supplementation increases T helper 1 cytokine production in old mice infected with influenza virus. Immunology 100:487–493 [View Article][PubMed]
     [Google Scholar]
  16. Hayek M. G., Taylor S. F., Bender B. S., Han S. N., Meydani M., Smith D. E., Eghtesada S., Meydani S. N. 1997; Vitamin E supplementation decreases lung virus titers in mice infected with influenza. J Infect Dis 176:273–276 [View Article][PubMed]
     [Google Scholar]
  17. Heinzerling R. H., Tengerdy R. P., Wick L. L., Lueker D. C. 1974a; Vitamin E protects mice against Diplococcus pneumoniae type I infection. Infect Immun 10:1292–1295[PubMed]
     [Google Scholar]
  18. Heinzerling R. H., Nockels C. F., Quarles C. L., Tengerdy R. P. 1974b; Protection of chicks against E. coli infection by dietary supplementation with vitamin E. Proc Soc Exp Biol Med 146:279–283[PubMed] [CrossRef]
     [Google Scholar]
  19. Jones R. N. 2010; Microbial etiologies of hospital-acquired bacterial pneumonia and ventilator-associated bacterial pneumonia. Clin Infect Dis 51:Suppl. 1S81–S87 [View Article][PubMed]
     [Google Scholar]
  20. Kaminogawa S., Nanno M. 2004; Modulation of immune functions by foods. Evid Based Complement Alternat Med 1:241–250 [View Article][PubMed]
     [Google Scholar]
  21. Lowy F. D. 2003; Antimicrobial resistance: the example of Staphylococcus aureus . J Clin Invest 111:1265–1273[PubMed] [CrossRef]
     [Google Scholar]
  22. Meydani S. N., Han S. N., Wu D. 2005; Vitamin E and immune response in the aged: molecular mechanisms and clinical implications. Immunol Rev 205:269–284 [View Article][PubMed]
     [Google Scholar]
  23. Moriguchi S., Itoh T. 1997; Vitamin E enhances T cell differentiation through increased epithelial cell function in rat thymus. Nutr Res 17:873–883 [View Article]
     [Google Scholar]
  24. Nichols R. L., Graham D. R., Barriere S. L., Rodgers A., Wilson S. E., Zervos M., Dunn D. L., Kreter B. for the Synercid Skin and Skin Structure Infection Group 1999; Treatment of hospitalized patients with complicated Gram-positive skin and skin structure infections: two randomized, multicentre studies of quinupristin/dalfopristin versus cefazolin, oxacillin or vancomycin. J Antimicrob Chemother 44:263–273 [View Article][PubMed]
     [Google Scholar]
  25. Owens C. D., Stoessel K. 2008; Surgical site infections: epidemiology, microbiology and prevention. J Hosp Infect 70:Suppl. 23–10 [View Article][PubMed]
     [Google Scholar]
  26. Pace J. L., Yang G. 2006; Glycopeptides: update on an old successful antibiotic class. Biochem Pharmacol 71:968–980 [View Article][PubMed]
     [Google Scholar]
  27. Peterson L. R. 2008; A review of tigecycline – the first glycylcycline. Int J Antimicrob Agents 32:Suppl. 4S215–S222 [View Article][PubMed]
     [Google Scholar]
  28. Provinciali M. 2009; Immunosenescence and cancer vaccines. Cancer Immunol Immunother 58:1959–1967 [View Article][PubMed]
     [Google Scholar]
  29. Provinciali M., Di Stefano G., Fabris N. 1992; Optimization of cytotoxic assay by target cell retention of the fluorescent dye carboxyfluorescein diacetate (CFDA) and comparison with conventional 51Cr-release assay. J Immunol Methods 155:19–24 [View Article]
     [Google Scholar]
  30. Sheridan P. A., Beck M. A. 2008; The immune response to herpes simplex virus encephalitis in mice is modulated by dietary vitamin E. J Nutr 138:130–137[PubMed]
     [Google Scholar]
  31. Simões M., Rocha S., Coimbra M. A., Vieira M. J. 2008; Enhancement of Escherichia coli and Staphylococcus aureus antibiotic susceptibility using sesquiterpenoids. Med Chem 4:616–623 [View Article][PubMed]
     [Google Scholar]
  32. Simonetti O., Cirioni O., Ghiselli R., Goteri G., Scalise A., Orlando F., Silvestri C., Riva A., Saba V. et al. other authors 2008; RNAIII-inhibiting peptide enhances healing of wounds infected with methicillin-resistant Staphylococcus aureus . Antimicrob Agents Chemother 52:2205–2211 [View Article][PubMed]
     [Google Scholar]
  33. Steenbergen J. N., Alder J., Thorne G. M., Tally F. P. 2005; Daptomycin: a lipopeptide antibiotic for the treatment of serious Gram-positive infections. J Antimicrob Chemother 55:283–288 [View Article][PubMed]
     [Google Scholar]
  34. Steenbergen J. N., Mohr J. F., Thorne G. M. 2009; Effects of daptomycin in combination with other antimicrobial agents: a review of in vitro and animal model studies. J Antimicrob Chemother 64:1130–1138 [View Article][PubMed]
     [Google Scholar]
  35. Stevens D. L. 2009; Treatments for skin and soft-tissue and surgical site infections due to MDR Gram-positive bacteria. J Infect 59:Suppl. 1S32–S39 [View Article][PubMed]
     [Google Scholar]
  36. Tengerdy R. P. 1990; The role of vitamin E in immune response and disease resistance. Ann N Y Acad Sci 587:24–33[PubMed]
     [Google Scholar]
  37. Tsalie E., Kouzi K., Poutahidis T., Abas Z., Sarris K., Iliadis N., Kaldrymidou E. 2006; Effect of vitamin E nutritional supplementation on the pathological changes induced in the ileum of rabbits by experimental infection with enteropathogenic Escherichia coli . J Comp Pathol 134:308–319 [View Article][PubMed]
     [Google Scholar]
  38. Tvedten H. W., Whitehair C. K., Langham R. F. 1973; Influence of vitamins A and E on gnotobiotic and conventionally maintained rats exposed to Mycoplasma pulmonis . J Am Vet Med Assoc 163:605–612[PubMed]
     [Google Scholar]
  39. Zampieri N., Zuin V., Burro R., Ottolenghi A., Camoglio F. S. 2010; A prospective study in children: pre- and post-surgery use of vitamin E in surgical incisions. J Plast Reconstr Aesthet Surg 63:1474–1478 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.032516-0
Loading
Vitamin E improves the in vivo efficacy of tigecycline and daptomycin in an animal model of wounds infected with meticillin-resistant Staphylococcus aureus
J. Med. Microbiol. 60, 1806 (2011); https://doi.org/10.1099/jmm.0.032516-0
/content/journal/jmm/10.1099/jmm.0.032516-0
/content/journal/jmm/10.1099/jmm.0.032516-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error