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Volume 68, Issue 2

sp. nov., isolated from a pigeon in Ma’anshan, China Free

Abstract

A Gram-negative, facultatively anaerobic bacillus, strain 08MAS2615, was isolated from the flesh of a pigeon specimen collected in Ma’anshan, Anhui province, China. Phylogenetic analysis of 16S rRNA gene sequences confirmed that strain 08MAS2615 belonged to the genus , and formed an independent branch which was clearly separated from the other six known species of Strain 08MAS2615 was more closely related to ATCC 29905 and NCTC 12737 than other species. Similar independent phylogenetic results were obtained using gene sequence analysis, whereas strain 08MAS2615 clustered near the species of JS9 and N5/687. Furthermore, the genome-wide core-single nucleotide polymorphism-based phylogenetic tree confirmed that strain 08MAS2615 formed a monophyletic and robust clade. Based on whole-genome sequences, the range of DNA–DNA hybridization and average nucleotide identity between strain 08MAS2615 and the six species were 25.5–48.8 % and 82.8–92.9 %, respectively, less than the proposed cutoff level for species delineation, i.e. 70 and 95 %. In addition, the major cellular fatty acid profile of strain 08MAS2615 was C (12.4 %), C (23.8 %), Ccyclo (14.4 %), summed feature 2 (Ciso I/C 3-OH) (11.0 %), summed feature 3 (Cω7/ω6) (18.5 %) and summed feature 8 (Cω6) (18.6 %). On the basis of these results, strain 08MAS2615 represents a novel species of the genus , for which the name sp. nov. is proposed with strain 08MAS2615 (=DSM 104686=CGMCC 1.15982) designated as the species type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): average nucleotide identity , in silico DDH , Proteus and rpoB
© 2018 IUMS
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2018-02-01
2024-04-20
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Proteus columbae sp. nov., isolated from a pigeon in Ma’anshan, China
Int J Syst Evol Microbiol 68, 552 (2018); https://doi.org/10.1099/ijsem.0.002541
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