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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 69, Issue 6

Pseudomonas urumqiensis sp. nov., isolated from rhizosphere soil of Alhagi sparsifolia Free

Abstract

A motile, Gram-stain-negative, fusiform-shaped bacterium, designated strain T3, was isolated from rhizosphere soil of Alhagi sparsifolia, collected from Xinjiang, PR China. Strain T3 grew at 15–42 °C, pH 4–9 and 1–6 % (w/v) NaCl concentrations. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain T3 belonged to the genus Pseudomonas and showed highest similarity of 98.6 % to Pseudomonas azotifigens JCM 12708, followed by Pseudomonas balearica DSM 6083 (97.8 %), Pseudomonas matsuisoli JCM 30078 (97.7 %), Pseudomonas furukawaii KF707 (97.7 %), Pseudomonas tarimensis CCTCC AB 2013065 (97.3 %) and Pseudomonas indica DSM 14015 (97.1 %). Analysis based on concatenated gene sequences of 16S rRNA, rpoB and gyrB further confirmed the phylogenetic assignment of strain T3. The Genome-to-Genome Distance Calculator results for P. azotifigens JCM12708 and P. balearica DSM 6083 were 28.7±4.4% and 24.1±2.4 %, and the average nucleotide identity scores were 81.3 and 78.1 %. The major polar lipids of strain T3 were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The predominant quinone was Q-9. The major fatty acids comprised summed feature 8 (C18 : 1 ω6c/C18 : 1 ω7c; 37.7 %), summed feature 3 (C16 : 1ω6c/C16 : 1ω7c; 28.2 %), C16 : 0 (15.6 %), C12 : 0 (7.8 %), C10 : 03-OH (3.0 %) and C12 : 03-OH (2.6 %). The G+C content of the genomic DNA of the type strain was 65.3 mol%. It is obvious from the phylogenetic, phenotypic and chemotaxonomic data that strain T3 represents a novel species of the genus Pseudomonas , for which the name Pseudomonas urumqiensis sp. nov., is proposed. The type strain is T3 (=ACCC 60124=JCM 32830).

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Keyword(s): Alhagi sparsifolia , bacterium , Pseudomonas urumqiensis and rhizosphere soil
© 2019 IUMS
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2019-04-05
2024-04-20
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Pseudomonas urumqiensis sp. nov., isolated from rhizosphere soil of Alhagi sparsifolia
Int J Syst Evol Microbiol 69, 1760 (2019); https://doi.org/10.1099/ijsem.0.003390
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