1887

Abstract

SUMMARY

A new technique based upon the inhibition of the peroxidase-labelled antibody staining (PLAS) has been used to type (HVH) antibodies in four groups of human sera taken from patients with one or both types of HVH infection and in mixtures of different proportions of type 1 and type 2 antisera. The results were compared with those of the microneutralisa-tion (MN) test and the indirect haemagglutination (IHA) inhibition test. The sensitivity and specificity of the three methods were identical for sera containing only one type of HVH antibody. The MN test was slightly more sensitive than the other tests for detecting small amounts of HVH-1 antibody mixed with large amounts of HVH-2 antibody. Nevertheless, the PLAS inhibition technique was far more rapid and it would seem a satisfactory alternative to the IHA inhibition test for HVH antibody typing.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-10-3-309
1977-08-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/jmm/10/3/medmicro-10-3-309.html?itemId=/content/journal/jmm/10.1099/00222615-10-3-309&mimeType=html&fmt=ahah

References

  1. Adam E., Kaufman R. H., Melnick J. L., Levy A. H., Rawls W. E. 1973; Seroepidemiologic studies of herpesvirus type 2 and carcinoma of the cervix. IV. Dysplasia and carcinoma in situ. Am. J. Epidem 98:77
    [Google Scholar]
  2. Avrameas S., Ternynck T. 1971; Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry 8:1175
    [Google Scholar]
  3. Back A. F., Schmidt N. J. 1974; Typing Herpesvirus hominis antibodies and isolates by inhibition of the indirect hemagglutination reaction. Appl. Microbiol 28:400
    [Google Scholar]
  4. Bernstein M. T., Stewart J. A. 1971; Method for typing antisera to Herpesvirus hominis by indirect hemagglutination inhibition. Appl. Microbiol 21:680
    [Google Scholar]
  5. Dowdle W. R., Nahmias A. J., Harwell R. W., Pauls F. P. 1967; Association of antigenic type of Herpesvirus hominis with site of viral recovery. J. Immun 99:974
    [Google Scholar]
  6. Fazekas de St, Groth S. 1961; Evaluation of quantal neutralization tests. Nature, Lond 191:891
    [Google Scholar]
  7. Forghani B., Schmidt N. J., Lennette E. H. 1975; Solid-phase radioimmunoassay for typing herpes simplex viral antibodies in human sera. J. clin. Microbiol 2:410
    [Google Scholar]
  8. Gerna G., McCloud C. J., Chambers R. W. 1976a; Immunoperoxidase technique for detection of antibodies to human cytomegalovirus. J. clin. Microbiol 3:364
    [Google Scholar]
  9. Gerna G., Vasquez A., McCloud C. J., Chambers R. W. 1976b; The immunoperoxidase technique for rapid human cytomegalovirus identification. Archs Virol 50:311
    [Google Scholar]
  10. Melnick J. L., Courtney R. J., Powell K. L., Schaffer P. A., Benyesh-Melnick M., Dreesman G. R., Anzai T., Adam E. 1976; Studies on herpes simplex virus and cancer. Cancer Res 36:845
    [Google Scholar]
  11. Nahmias A. J., Josey W. E., Naib Z. M., Luce C. F., Duffey A. 1970; Antibodies to Herpesvirus hominis types 1 and 2 in humans. I. Patients with genital herpetic infections. Am. J. Epidem 91:539
    [Google Scholar]
  12. Naib Z. M., Nahmias A. J., Josey W. E., Kramer J. H. 1969; Genital herpetic infection associated with cervical dysplasia and carcinoma. Cancer, Philad 23:940
    [Google Scholar]
  13. Pauls F. P., Dowdle W. R. 1967; A serologic study of Herpesvirus hominis strains by microneutralization tests. J. Immun 98:941
    [Google Scholar]
  14. Rawls W. E., Tompkins W. A. F., Figueroa M. E., Melnick J. L. 1968; Herpes virus type 2: association with carcinoma of the cervix. Science, N. Y 161:1255
    [Google Scholar]
  15. Reed L. J., Muench H. 1938; A simple method of estimating fifty per cent end points. Am. J. Hyg 27:493
    [Google Scholar]
  16. Skinner G. R. B., Hartley C., Whitney J. E. 1976; Detection of type-specific antibody to herpes simplex virus type 1 and 2 in human sera by complement-fixation tests. Archs Virol 50:323
    [Google Scholar]
  17. Smith J. W., Adam E., Melnick J. L., Rawls W. E. 1972a; Use of the 11Cr release test to demonstrate patterns of antibody response in humans to herpes virus types 1 and 2. J. Immun 109:554
    [Google Scholar]
  18. Smith J. W., Lowry S. P., Melnick J. L., Rawls W. E. 1972b; Antibodies to surface antigens of herpesvirus type 1-and type 2-infected cells among women with cervical cancer and control women. Infect. Immun 5:305
    [Google Scholar]
  19. Stalder H., Oxman M. N., Herrmann K. L. 1975; Herpes simplex virus microneutralization: a simplification of the test. J. inf. Dis 131:423
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-10-3-309
Loading
/content/journal/jmm/10.1099/00222615-10-3-309
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error