Cytotoxic activity in broth-culture filtrates of Campylobacter pylori
Leunk, R. D. and Johnson, P. T. and David, B. C. and Kraft, W. G. and Morgan, D. R.,, 26, 93-99 (1988),
doi = https://doi.org/10.1099/00222615-26-2-93,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= SUMMARY Broth-culture filtrates of Campylobacter pylori induced non-lethal cytopathic effects in vitro in 7 of 9 mammalian cell lines tested. Transmission electronmicroscopy revealed that the response consisted of intracellular vacuolisation. Intestine 407 cells were among the most responsive and were used for routine assay. About 55% of isolates of C. pylori tested, originating from four geographic regions worldwide, produced cytotoxic activity. The activity was neutralisable by specific antisera to broth-culture filtrates or to sonicated bacteria but not by antisera to other bacterial preparations. Cytotoxic activity was heat-labile (70°C for 30 min), was protease-sensitive and ammonium-sulphate precipitable. It did not pass through an ultrafiltration membrane with a nominal mol.-wt limit of 100 × 103. It was concluded that C. pylori can produce a factor that alters cultured cells in vitro. The relevance of this factor to the pathogenesis of gastritis associated with C. pylori remains to be determined.,
language=,
type=