1887

Abstract

Surmmary

The most useful and reliable serological investigations for the diagnosis of current infection, including reinfection, were investigated. Paired sera and respiratory specimens from 115 patients with lower respiratory tract symptoms were examined for evidence of current infection by serological response, as measured by complement-fixation and indirect immunofluorescence tests for specific IgM, IgA and IgG, and also by culture of from respiratory material. Specific IgM was not always detectable in cases where other criteria indicated current or recent infection. On the basis of the present results, it is postulated that primary infection and reinfection may be differentiated by the presence or absence of specific IgM in the presence of elevated specific IgA levels and, therefore, that estimation of both IgM and IgA is necessary for the maximal detection of current infection, including reinfections. Specific IgG levels remained elevated for many weeks and were not useful diagnostically.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-33-4-253
1990-12-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/33/4/medmicro-33-4-253.html?itemId=/content/journal/jmm/10.1099/00222615-33-4-253&mimeType=html&fmt=ahah

References

  1. Ali NJ, Sillis M, Andrews BE, Jenkins PF, Harrison BDW. The clinical spectrum and diagnosis of Mycoplasma pneumoniae infections. Q J Med 1986; 58:227241–251
    [Google Scholar]
  2. Pönkä A, Pönkä T, Sama S, Penttinen K. Questionable specificity of lipid antigen in the Mycoplasma pneumoniae complement-fixation test in patients with extrapulmonary manifestations. J Infect 1981; 3:332–338
    [Google Scholar]
  3. Kleemola M, Kayhty H. Increase in titres of antibodies to Mycoplasma pneumoniae in patients with purulent meningitis. J Infect Dis 1982; 146:284–288
    [Google Scholar]
  4. Svehag SE. Antibodies to biologically active molecules. Proc Fed Eur Biochem Soc, Vienna 1965; 1:301–349
    [Google Scholar]
  5. Wreghitt TG, Sillis M. A μ-capture ELISA technique for detecting Mycoplasma pneumoniae IgM: comparison with indirect immunofluorescence and indirect ELISA. JHyg (Camb) 1985; 94:217–227
    [Google Scholar]
  6. Foy HM, Kenny GE, Sefi R, Ochs HD, Allan ID. Second attacks of pneumonia due to Mycoplasma pneumoniae. J Infect Dis 1977; 135:673–677
    [Google Scholar]
  7. Foy HM, Kenny GE, Cooney MK, Allan ID, van Belle G. Natural acquired immunity to pneumonia due to Mycoplasma pneumoniae. J Infect Dis 1983; 147:967–973
    [Google Scholar]
  8. Cradock-Watson JE, Ridehalgh MKS, Anderson MJ, Pattison JR. Outcome of asymptomatic infection with rubella vims during pregnancy. J Hyg (Camb) 1981; 87:147–154
    [Google Scholar]
  9. Bradstreet CMP, Taylor CED. Technique of complement- fixation test applicable to the diagnosis of virus diseases. Monthly Bull Min Health PHLS 1962; 21:96–104
    [Google Scholar]
  10. Woodhams A, Mead G. A comparison between pancreatin and iV-acetyl-L-cysteine as sputum liquefying agents for the culture of organisms. Tubercle 1965; 46:224–226
    [Google Scholar]
  11. Klieneberger-Nobel E. Pleuropneumonia-like organisms (PPLO). Mycoplasmataceae London: Academic Press; 1962; IV58
    [Google Scholar]
  12. Chamberlain P, Saeed A A. A study of the specific IgM antibody response in Mycoplasma pneumoniae infection in man. J Hyg (Camb) 1983; 90:207–211
    [Google Scholar]
  13. Foy HM, Grayston JT, Kenny GE, Alexander ER, McMahan R. Epidemiology of Mycoplasma pneumoniae infection in families. JAMA 1966; 197:859–866
    [Google Scholar]
  14. Biberfeld G. Antibody responses in Mycoplasma pneumoniae infection in relation to serum immunoglobulins, especially IgM. Acta Pathol Microbiol Scand B 1971; 79:620–634
    [Google Scholar]
  15. Fernald G, Collier A, Clyde WA. Respiratory infections due to Mycoplasma pneumoniae in infants and children. Pediatrics 1975; 55:327–335
    [Google Scholar]
  16. Collier AM, Clyde WA, Denny FW. Biologic effects of Mycoplasma pneumoniae and other mycoplasmas from man on hamster tracheal organ culture. Proc Soc Exp Biol Med 1969; 132:1153–1158
    [Google Scholar]
  17. Skaug K, Eng J, Örstavik I, Haug KW. The diagnostic value of determination of IgM antibodies against Mycoplasma pneumoniae by indirect immunofluorescent antibody test. Acta Pathol Microbiol Scand B 1976; 84:170–176
    [Google Scholar]
  18. Cordero L, Cuadrado R, Hall CB, Horstmann DM. Primary atypical pneumonia: an epidemic caused by Mycoplasma pneumoniae. J Ped 1967; 71:1–12
    [Google Scholar]
  19. Taylor-Robinson D, Soběslavský OJ, Jensen KE, Senterfit LB, Chanock RM. Serologic response to Mycoplasma pneumoniae infection. I. Evaluation of immunofluorescence, complement fixation, indirect hemagglutination, and tetrazolium reduction inhibition tests for the diagnosis of infection. Am J Epidemiol 1966; 83:287–298
    [Google Scholar]
  20. Umetsu M, Ogawa S, Chiba S, Nakao T. Immune responses in Mycoplasma pneumoniae infections. Tohoku J Exp Med 1975; 116:213–218
    [Google Scholar]
  21. Grauballe PC, Hornsleth A, Hjelt K, Krasilnikoff PA. Detection by ELISA of immunoglobulin G subclass—specific antibody response in rotavirus infections in children. J Med Virol 1986; 18:277–281
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-33-4-253
Loading
/content/journal/jmm/10.1099/00222615-33-4-253
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error