1887

Abstract

Summary

A new DNA probe assay (PACE 2, Gen-probe) was compared with cell culture for the detection of in 909 women attending the Royal Women’s Hospital, Melbourne, Victoria. The DNA probe assay had a sensitivity of 86.2%, a specificity of 99.9% and a positive predictive value of 96.2% in a population with 3.2% prevalence, indicating that it may be a suitable alternative to culture for the detection of in specimens from the genital tract.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-35-3-159
1991-09-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/jmm/35/3/medmicro-35-3-159.html?itemId=/content/journal/jmm/10.1099/00222615-35-3-159&mimeType=html&fmt=ahah

References

  1. Ridgway GL. The laboratory diagnosis of chlamydial infection. Oriel D, Ridgway G, Schachter J. et al.eds Chlamydial infections Cambridge: Cambridge University Press; 1986539–549
    [Google Scholar]
  2. Lipkin ES, Moncada JV, Shafer M-A, Wilson TE, Schachter J. Comparison of monoclonal antibody staining and culture in diagnosing cervical chlamydial infection. J Clin Microbiol 1986; 23:114–117
    [Google Scholar]
  3. Lees MI, Newman DM, Garland SM. Simplified culture procedure for large-scale screening for Chlamydia trachomatis infections. J Clin Microbiol 1988; 26:1428–1430
    [Google Scholar]
  4. Peterson EM, Oda R, Alexander R, Greenwood JR, de la Maza LM. Molecular techniques for the detection of Chlamydia trachomatis. J Clin Microbiol 1989; 27:2359–2363
    [Google Scholar]
  5. Woods GL, Young A, Scott JC, Blair TMH, Johnson AM. Evaluation of a nonisotopic probe for detection of Chlamydia trachomatis in endocervical specimens. J Clin Microbiol 1990; 28:370–372
    [Google Scholar]
  6. LeBar W, Herschman B, Jemal C, Pierzchala J. Comparison of DNA probe, monoclonal antibody enzyme immunoassay, and cell culture for the detection of Chlamydia trachomatis. J Clin Microbiol 1989; 27:826–828
    [Google Scholar]
  7. Pao CC, Lin S-S, Yang T-E, Soong Y-K, Lee P-S, Lin J-Y. Deoxyribonucleic acid hybridization analysis for the detection of urogenital Chlamydia trachomatis infections in women. Am J Obstet Gynecol 1987; 156:195–199
    [Google Scholar]
  8. Horn JE, Quinn T, Hammer M, Palmer L, Falkow S. Use of nucleic acid probes for the detection of sexually transmitted infectious agents. Diagn Microbiol Infect Dis 1986; 4:Suppl 3101S–109S
    [Google Scholar]
  9. Mumtaz G, Mellars BJ, Ridgway GL, Oriel JD. Enzyme immunoassay for the detection of Chlamydia trachomatis antigen in urethral and endocervical swabs. J Clin Pathol 1985; 38:740–742
    [Google Scholar]
  10. Mahoney J, Castriciano S, Sellors J. et al. Diagnosis of Chlamydia trachomatis genital infections by cell culture and two enzyme immunoassays detecting different chlamydial antigens. J Clin Microbiol 1989; 27:1934–1938
    [Google Scholar]
  11. Tilton RC, Judson FN, Barnes RC, Gruninger RP, Ryan RW, Steingrimsson O. Multicenter comparative evaluation of two rapid microscopic methods and culture for detection of Chlamydia trachomatis in patient specimens. J Clin Microbiol 1988; 26:167–170
    [Google Scholar]
  12. Lees M, Newnan D, Garland SM. Detection of Chlamydia trachomatis by direct immunofluorescence and culture in genital specimens of symptomatic and asymptomatic women. Venereology 1990; 3:28–31
    [Google Scholar]
  13. Smith JW, Rogers RE, Katz BP. et al. Diagnosis of chlamydial infection in women attending antenatal and gynecologic clinics. J Clin Microbiol 1987; 25:868–872
    [Google Scholar]
  14. Chernesky MA, Mahoney JB, Castriciano S. et al. Detection of Chlamydia trachomatis antigens by enzyme immunoassay and immunoflurescence in genital specimens from symptomatic and asymptomatic men and women. J Infect Dis 1986; 154:141–148
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-35-3-159
Loading
/content/journal/jmm/10.1099/00222615-35-3-159
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error