The production of porphyrins from δ-aminolaevulinic acid by Haemophilus parainfluenzae
Luppa, P. and Jacob, K. and Ehret, W.,, 39, 262-267 (1993),
doi = https://doi.org/10.1099/00222615-39-4-262,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= Summary Porphyrin production by the haemin-independent Haemophilus parainfluenzae in the diagnostic porphyrin test, which determines the X-factor requirement in Haemophilus spp., was analysed quantitatively by applying modern high-performance liquid chromatographic (HPLC) methods. Ion-pair reversed-phase HPLC enabled the simultaneous separation of all porphyrin intermediates and their isomers of haem biosynthesis produced by the bacteria. The pH-dependence of porphyrin production and the respective composition of the porphyrins within the bacterial cells and in the supernate were investigated. A pH optimum of 6.9—8.0 for the production of porphyrins was found and there were marked differences in the porphyrin profiles at different pH values.,
language=,
type=