1887

Abstract

Summary

A prospective study of the serological response to natural infection with and was performed in an experimental flock of sheep. A preliminary investigation with immunoblotting techniques showed that lambs infected with virulent spp. produced antibodies to several yersinia outer-membrane proteins (yops) encoded by a virulence plasmid (pYV) of or . Thereafter, an enzyme immunoassay (EIA) was developed to measure antibodies to yops. Criteria for interpreting the EIA were established by examining sera from a negative control population of lambs which had not been infected with spp. since birth. Test samples comprised 25 pairs of pre- and post-infection sera from animals with bacteriologically proven infections with spp. The results showed that infection of lambs with pYV-bearing strains of or invariably evoked a significant antibody response to yops, even though all the infections were subclinical. No animal infected with so-called “environmental”, pYV -negative spp. seroconverted to yops. EIA with yops as antigen provided a sensitive and specific means to diagnose subclinical infection of lambs with virulent spp.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-39-4-268
1993-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/39/4/medmicro-39-4-268.html?itemId=/content/journal/jmm/10.1099/00222615-39-4-268&mimeType=html&fmt=ahah

References

  1. Cornelis G, Laroche Y, Balligand G, Sory M-P, Wauters G. Yersinia enterocolitica, a primary model for bacterial invasiveness. Rev Infect Dis 1987; 9:64–87
    [Google Scholar]
  2. Portnoy DA, Martinez RJ. Role of a plasmid in the patho genicity of Yersinia species. Curr Top Microbiol Immunol 1985; 118:29–51
    [Google Scholar]
  3. Bliska JB, Guan K, Dixon JE, Falkow S. Tyrosine phosphate hydrolysis of host proteins by an essential Yersinia virulence determinant. Proc Natl Acad Sci USA 1991; 88:1187–1191
    [Google Scholar]
  4. Kapperud G, Namork E, Skurnik M, Nesbakken T. Plasmid-mediated surface fibrillae of Yersinia pseudotuberculosis and Yersinia enterocolitica: relationship to the outer membrane protein YOP1 and possible importance for pathogenesis. Infect Immun 1987; 55:2247–2254
    [Google Scholar]
  5. Rosqvist R, Forsberg A, Rimpilainen N, Bergman T, Wolf-Watz H. The cytotoxic protein YopE of Yersinia obstructs the primary host defence. Mol Microbiol 1990; 4:657–667
    [Google Scholar]
  6. Bolin I, Forsberg A, Norlander L, Skurnik M, Wolf-Watz H. Identification and mapping of the temperature-inducible plasmid-encoded proteins of Yersinia spp. Infect Immun 1988; 56:343–348
    [Google Scholar]
  7. Heesemann J, Gross U, Schmidt N, Laufs R. Immunochemical analysis of plasmid-encoded proteins released by entero- pathogenic Yersinia sp. grown in calcium-deficient media. Infect Immun 1986; 54:561–567
    [Google Scholar]
  8. Heesemann J, Keller C, Morowa R, Schmidt N, Siemens HJ, Laufs R. Plasmids of human strains of Yersinia enterocolitica : molecular relatedness and possible importance for pathogenesis. J Infect Dis 1983; 147:107–115
    [Google Scholar]
  9. Robins-Browne RM, Miliotis MD, Cianciosi S, Miller VL, Falkow S, Morris JG. Evaluation of DNA colony hybridization and other techniques for detection of virulence in Yersinia species. J Clin Microbiol 1989; 27:644–650
    [Google Scholar]
  10. Michiels T, Wattiau P, Brasseur R, Ruysschaert J-M, Cornelis G. Secretion of yop proteins by yersiniae. Infect Immun 1990; 58:2840–2849
    [Google Scholar]
  11. Heesemann J, Eggers C, Schroder J, Laufs R. Serological diagnosis of yersiniosis by the immunoblot technique using plasmid-encoded antigens of Y. enterocolitica. In: Simon C, Wilkinson P. (eds) Diagnosis of infectious diseases. New aspects Stuttgart: Schattauer; 198679–88
    [Google Scholar]
  12. Hoogkamp-Korstanje JAA, de Koning J, Heesemann J, Festen JJM, Houtman PM, van Oyen PLM. Influence of antibiotics on IgA and IgG response and persistence of Yersinia enterocolitica in patients with Yersinia-associated spon-dylarthropathy. Infection 1992; 20:53–57
    [Google Scholar]
  13. Maki-Ikola O, Heesemann J, Lahesmaa R, Toivanen A, Granfors K. Combined use of released proteins and lipopolysaccharide in enzyme-linked immunosorbent assay for serologic screening of Yersinia infections. J Infect Dis 1991; 163:409–412
    [Google Scholar]
  14. Stahlberg TH, Heesemann J, Granford K, Tovainen A. Immunoblot analysis of IgM, IgG and IgA responses to plasmid encoded released proteins of Yersinia enterocolitica in patients with or without Yersinia-triggered reactive arthritis. Ann Rheum Dis 1989; 48:577–581
    [Google Scholar]
  15. Slee KJ, Skilbeck NW. Epidemiology of Yersinia pseudo tuberculosis and Y. enterocolitica infections in sheep in Australia. J Clin Microbiol 1992; 30:712–715
    [Google Scholar]
  16. Wauters G, Kandolo K, Janssens M. Revised biogrouping scheme of Yersinia enterocolitica. Contrib Microbiol Immunol 1987; 9:14–21
    [Google Scholar]
  17. Prpic JK, Robins-Browne RM, Davey RB. In vitro assessment of virulence in Yersinia enterocolitica and related species. J Clin Microbiol 1985; 22:105–10
    [Google Scholar]
  18. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  19. Bottone EJ, Sheehan DJ. Yersinia enterocolitica'. guidelines for serologic diagnosis of human infections. Rev Infect Dis 1983; 5:898–906
    [Google Scholar]
  20. WHO Expert Committee on Plague WHO Tech Rep Ser 1970; 447:1–25
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-39-4-268
Loading
/content/journal/jmm/10.1099/00222615-39-4-268
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error