@article{mbs:/content/journal/jmm/10.1099/00222615-40-1-31, author = "Said, Bengü and Scotland, Sylvia M. and Rowe, B.", title = "The use of gene probes, immunoassays and tissue culture for the detection of toxin in Vibrio cholerae non-O1", journal= "Journal of Medical Microbiology", year = "1994", volume = "40", number = "1", pages = "31-36", doi = "https://doi.org/10.1099/00222615-40-1-31", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-40-1-31", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "Summary. Vibrio cholerae non-O1 strains were screened for the presence of cholera enterotoxin (CT) genes by means of digoxigenin-labelled polynucleotide CTA and CTB probes. In-vitro production of CT was investigated by the Y1 mouse adrenal cell assay, enzyme-linked immunosorbent assay (ELISA) and a commercial, reversed passive latex agglutination (RPLA) kit. Only two (0·25%) of 790 strains tested gave positive results with the CTA and CTB probes. The production of other bacterial cytotoxin(s) made it impossible to use the characteristic cell-rounding effect on Y1 cells for the detection of CT. CT production by the probe-positive strains was confirmed by the immunoassays. Two hundred and fifty-two of the 788 probe-negative strains were tested by both cell assay and immunoassays. Of these, 90% produced cytotoxin(s) in the cell assay. In addition, 37% gave positive results in CT-ELISA, but negative results with LT-ELISA and VET-RPLA. These results indicate the presumed presence of a toxin in V. cholerae non-O1 that is able to bind GM1 and react with antisera to CT, but which is not identical to CT.", }