1887

Abstract

Surmmary

Administration of either gentamicin or amikacin induced an increase in the number of amikacin-resistant (AR) isolates of certain Enterobacteriaceae and species in a hospital in Buenos Aires. A total of 127 AR isolates was selected to study the molecular mechanisms of resistance involved. The (′)- gene was found by dot-blot hybridisation in every isolate. A gene different from (′)-(′)- and (′)- encoding the AAC(6′)-I activity was found in a 15-5-kb plasmid in spp. Plasmids from 27 Enterobacteriaceae contained an aac(′)- gene and 26 of these carried sequences related to the Tn1331 transposon, whereas one plasmid showed homology in another fragment of the Tn1331 transposase. Because plasmids bearing the (′)- gene were heterogeneous, dissemination of the (′)- gene may have been due to transposition of Tn1331 rather than the spread of an epidemic plasmid. The rate of AR isolates varied within each species in spite of the presence of Tn1331, and it is likely, therefore, that this transposon may not be the sole factor responsible for the observed variation. The (′)- gene (originally described in spp.) was found with high frequency (80%) in this population. Furthermore, this gene was found also in plasmids from 20% of other gram-negative organisms commonly involved in nosocomial infections in this hospital.

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1995-04-01
2024-03-28
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