1887

Abstract

Summary

The cultural characteristics of 112 serovars belonging to subspecies I–VI were examined on Rambach agar and SM-ID medium. Colonies showing the typical red coloration were seen with 100 of 112 serovars assayed on SM-ID, and with 87 of 112 on Rambach agar. Atypical colourless colonies were observed on Rambach agar with ONPG-negative serovars S. Choleraesuis, S. Isangi, S. Typhi S. Worthington and S. Yoff of the subspecies I, S. II 52:d:e, n, x, z of the subspecies II, 5. IV 6, 7: Z , z,:- and S. IV 11:g, z:-of the subspecies IV, and S. 40:z: belonging to 5. bongori (V) species. Atypical blue, blue-green, blue-violet or violet colonies were observed on both media with all the ONPG-positive serovars of the subspecies IIIa (four of four strains) and IIIb (six of six strains) and with one of the two ONPG-positive (out of five) strains of the subspecies VI. Four serovars of showed blue-green colonies on Rambach agar and typical red colonies on SM-ID, although they were all ONPG-positive. These results suggest that SM-ID medium is more sensitive than Rambach agar. However, the relatively low sensitivity of both media makes them suitable for use only in association with a traditional selective medium in both medical and environmental bacteriology, as well as for epidemiological purposes.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-43-1-68
1995-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/43/1/medmicro-43-1-68.html?itemId=/content/journal/jmm/10.1099/00222615-43-1-68&mimeType=html&fmt=ahah

References

  1. Rambach A. New plate medium for facilitated differentiation of Salmonella spp. from Proteus spp. and other enteric bacteria. Appl Environ Microbiol 1990; 56:301–303
    [Google Scholar]
  2. Poupart M. C., Mounier M., Denis F., Sirot J., Couturier C., Villeval F. A new chromogenic ready-to-use medium for Salmonella detection. 5th European Congress of Clinical Microbiology and Infectious Diseases Oslo. Norway: 1991
    [Google Scholar]
  3. Gruenewald R., Henderson R. W., Yappow S. Use of Rambach propylene glycol containing agar for identification of Salmonella spp. J Clin Microbiol 1991; 29:2354–2356
    [Google Scholar]
  4. Freydiere A. M., Gille Y. Detection of salmonellae by using Rambach agar and by a C8 esterase spot test. J Clin Microbiol 1991; 29:2357–2359
    [Google Scholar]
  5. Kühne H., Wonde B., Rabsch W., Reissbrodt R. Evaluation of Rambach agar for detection of Salmonella subspecies I to VI. Appl Environ Microbiol 1994; 60:749–751
    [Google Scholar]
  6. Le Minor L., Grimont P. A. D. Origine et repartition en serovars des souches de Salmonella isolees en France continental au cours des annees 1984 á 1987. Medicine et Maladies Infectieuses 1989; 19:12–17
    [Google Scholar]
  7. Center for Disease Control Salmonella surveillance—annual summary. Centers for Disease Control; Atlanta: 1989
    [Google Scholar]
  8. Dusch H., Altwegg M. Comparison of Rambach agar, SM-ID medium, and Hektoen enteric agar for primary isolation of non-typhi salmonellae from stool samples. J Clin Microbiol 1993; 31:410–412
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-43-1-68
Loading
/content/journal/jmm/10.1099/00222615-43-1-68
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error