1887

Abstract

The effect of biotin on toxin production by was examined in a defined medium. When toxin production by strain KZ 1647, which was isolated from a healthy adult, was examined in relation to its biotin requirement, it was found that with decreasing concentrations of biotin, bacterial growth was decreased, but production of both toxins A and B were remarkably increased, particularly with 0.05 nM biotin. The time course of production of both toxins in biotin-limited conditions was similar to that in biotin-enriched conditions. The biotin effect on toxin production was also observed in 15 other strains, suggesting that the effect occurs frequently amongst toxigenic strains. The biotin effect is discussed in relation to the pathogenesis of colitis.

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1996-02-01
2024-04-19
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References

  1. George W. L., Sutter V. L., Goldstein E. J. C., Ludwig S. L., Finegold S. M. Aetiology of antimicrobial-agent-associated colitis. Lancet 1978; 1:802–803
    [Google Scholar]
  2. Larson H. E., Price A. B., Honour P., Borriello S. P. Clostridium difficile and the aetiology of pseudomembranous colitis. Lancet 1978; 1:1063–1066
    [Google Scholar]
  3. Bartlett J. G., Chang Y. W., Gurwith M., Gorbach S. L., Onderdonk A. B. Antibiotic-associated pseudomembranous colitis due to toxin-producing clostridia. N Engl J Med 1978; 298:531–534
    [Google Scholar]
  4. Lyerly D. M., Krivan H. C., Wilkins T. D. Clostridium difficile: its disease and toxins. Clin Microbiol Rev 1988; 1:1–18
    [Google Scholar]
  5. Sullivan N. M., Pellett S., Wilkins T. D. Purification and characterization of toxins A and B of Clostridium difficile . Infect Immun 1982; 35:1032–1040
    [Google Scholar]
  6. Banno Y., Kobayashi T., Kono H., Watanabe K., Ueno K., Nozawa Y. Biochemical characterization and biologic actions of two toxins (D-l and D-2) from Clostridium difficile . Rev Infect Dis 1984; 6: Suppl 1S11–S20
    [Google Scholar]
  7. Haslam S. C., Ketley J. M., Mitchell T. J., Stephen J., Burdon D. W., Candy D. C. A. Growth of Clostridium difficile and production of toxins A and B in complex and defined media. J Med Microbiol 1986; 21:293–297
    [Google Scholar]
  8. Yamakawa K., Kamiya S., Meng X. Q., Karasawa T., Nakamura S. Toxin production by Clostridium difficile in a defined medium with limited amino acids. J Med Microbiol 1994; 41:319–323
    [Google Scholar]
  9. Nakamura S., Mikawa M., Nakashio S. Isolation of Clostridium difficile from the feces and the antibody in sera of young and elderly adults. Microbiol Immunol 1981; 25:345–351
    [Google Scholar]
  10. Karasawa T., Ikoma S., Yamakawa K., Nakamura S. A defined growth medium for Clostridium difficile . Microbiology 1995; 141:371–375
    [Google Scholar]
  11. Redmond S. C., Ketley J. M., Mitchell T. J., Stephen J., Burdon,D. W., Candy D. C. Detection of Clostridium difficile enterotoxin (toxin A) by ELISA and other techniques. In Collins C. H., Grange J. M. (eds) Isolation and identification of microorganisms of medical and veterinary importance Society of Applied Bacteriology, Technical series No. 21 London: Academic Press; 1985237–250
    [Google Scholar]
  12. Kamiya S., Reed P. J., Borriello S. P. Purification and characterisation of Clostridium difficile toxin A by bovine thyroglobulin affinity chromatography and dissociation in denaturing conditions with or without reduction. J Med Microbiol 1989; 30:69–77
    [Google Scholar]
  13. Mekalanos J. J. Environmental signals controlling expression of virulence determinants in bacteria. J Bacteriol 1992; 174:1–7
    [Google Scholar]
  14. Kamiya S., Ogura H., Meng X. Q., Nakamura S. Correlation between cytotoxin production and sporulation in Clostridium difficile . J Med Microbiol 1992; 37:206–210
    [Google Scholar]
  15. George R. H., Johnson M., Youngs D., Burdon D. W. Induction of Clostridium difficile toxin by antibiotics. Proceedings of the 11th International Congress of Chemotherapy and 19th Interscience Conference on Antimicrobial Agent and Chemotherapy 1979955–956
    [Google Scholar]
  16. Honda T., Hemadez I., Katoh T., Miwatani T. Stimulation of enterotoxin production of Clostridium difficile by antibiotics. Lancet 1983; 1:655
    [Google Scholar]
  17. Nakamura S., Mikawa M., Tanabe N., Yamakawa K., Nishida S. Effect of clindamycin on cytotoxin production by Clostridium difficile . Microbiol Immunol 1982; 26:985–992
    [Google Scholar]
  18. Onderdonk A. B., Lowe B. R., Bartlett J. G. Effect of environmental stress of Clostridium difficile toxin levels during continuous cultivation. Appl Environ Microbiol 1979; 38:637–641
    [Google Scholar]
  19. Gottschalk G. Bacterial metabolism. 2nd edn New York: Springer-Verlag; 1986
    [Google Scholar]
  20. Dove C. H., Wang S.-Z. Price SB Molecular characterization of the Clostridium difficile toxin A gene. Infect Immun 1990; 58:480–488
    [Google Scholar]
  21. Johnson J. L., Phelps C., Barroso L., Roberts M. D., Lyerly D. M., Wilkins T. D. Cloning and expression of the toxin B gene of Clostridium difficile . Curr Microbiol 1990; 20:397–401
    [Google Scholar]
  22. Mickelsen O. Intestinal synthesis of vitamins in the non-ruminant. Vitam Horm 1956; 14:1–95
    [Google Scholar]
  23. Ohsugi M., Imanishi Y., Teraoka T., Nishimura K., Nakao S. Biosynthesis of biotin-vitamers by family Enterobacteriaceae. J Nutr Sci Vitaminol 1990; 36:447–56
    [Google Scholar]
  24. Bowden T. A., Mansberger A. R., Lykins L. E. Pseudomembranous enterocolitis: mechanism of restoring floral homeostasis. Am Surg 1981; 47:178–83
    [Google Scholar]
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