1887

Abstract

Nine hundred and twenty-five isolates from cases of diarrhoea in the United Kingdom and belonging to enteropathogenic (EPEC) O serogroups were examined for virulence properties. The tests included adhesion to HEp-2 cells, the fluorescence actin staining (FAS) test (which correlates with the ability to cause attaching and effacing lesions) and DNA hybridisations with probes to detect sequences for A ( attaching and effacing factor), EAF (EPEC adherence factor), verocytotoxins VT1 and VT2, enteroaggregative and diffusely adherent The O serogroups examined were 18, 26, 44, 55, 86, 111, 114, 119, 125, 126, 127, 128 and 142. Six hundred and sixty strains (71.4%) hybridised with at least one of the DNA probes. Over 80% of strains in O serogroups 26, 55, 119, 125, 127 and 142 and 41% of strains of serogroups 86, 111, 114, 126 and 128 hybridised with the probe and most showed localised attachment and were FAS-positive. However, <10% of these probepositive strains hybridised with the EAF probe. Eighty-four of 232 strains in O serogroups 44, 86, 111, and 126 were enteroaggregative. VT genes were detected in 57 of 402 strains in O serogroups 26, 55, 111 and 128. Identification of EPEC by serogrouping was shown to be an effective method of identifying strains with pathogenic potential, although the organisms were diverse in their properties.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-44-6-438
1996-06-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/44/6/medmicro-44-6-438.html?itemId=/content/journal/jmm/10.1099/00222615-44-6-438&mimeType=html&fmt=ahah

References

  1. Taylor J. Host specificity and enteropathogenicity of Escherichia coli. J Appl Bacteriol 1961; 24:316–325
    [Google Scholar]
  2. Gross R. J. Escherichia coli diarrhoea. In Smith G. R., Easmon C. S. F. (eds) Topley and Wilson’s Principles of bacteriology, virology and immunity 8th edn, Vol 3 London: Edward Arnold; 1990469–187
    [Google Scholar]
  3. Levine M. M. Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. J Infect Dis 1987; 155:377–389
    [Google Scholar]
  4. Law D. Adhesion and its role in the virulence of enteropathogenic Escherichia coli. Clin Microbiol Rev 1994; 7:152–173
    [Google Scholar]
  5. Cravioto A., Gross R. J., Scotland S. M., Rowe B. An adhesive factor found in strains of Escherichia coli belonging to the traditional infantile enteropathogenic serotypes. Curr Microbiol 1979; 3:95–99
    [Google Scholar]
  6. Knutton S., Baldwin T., Williams P. H., McNeish A. S. Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli. Infect Immun 1989; 57:1290–1298
    [Google Scholar]
  7. Baldini M. M., Nataro J. P., Kaper J. B. Localization of a determinant for HEp-2 adherence by enteropathogenic Escherichia coli. Infect Immun 1986; 52:334–336
    [Google Scholar]
  8. Jerse A. E., Yu J., Tall B. D., Kaper J. B. A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Natl Acad Sci USA 1990; 87:7839–7843
    [Google Scholar]
  9. Gross R. J., Rowe B. Serotyping of Escherichia coli. In Sussman M. (ed) The virulence of Escherichia coli. Reviews and methods London: Academic Press; 1985345–363
    [Google Scholar]
  10. Smith H. R., Scotland S. M., Stokes N., Rowe B. Examination of strains belonging to enteropathogenic Escherichia coli serogroups for genes encoding EPEC adherence factor and verocytotoxins. J Med Microbiol 1990; 31:235–240
    [Google Scholar]
  11. Scotland S. M., Smith H. R., Said B., Willshaw G. A., Cheasty T., Rowe B. Identification of enteropathogenic Escherichia coli isolated in Britain as enteroaggregative or as members of a subclass of attaching-and-effacing E. coli not hybridising with the EPEC adherence-factor probe. J Med Microbiol 1991; 35:278–283
    [Google Scholar]
  12. Scotland S. M., Willshaw G. A., Smith H. R., Said B., Stokes N., Rowe B. Virulence properties of Escherichia coli strains belonging to serogroups O26, O55, O111 and O128 isolated in the United Kingdom in 1991 from patients with diarrhoea. Epidemiol Infect 1993; 111:429–438
    [Google Scholar]
  13. Willshaw G. A., Smith H. R., Scotland S.M., Rowe B. Cloning of genes determining the production of Vero cytotoxin by Escherichia coli. J Gen Microbiol 1985; 131:3047–3053
    [Google Scholar]
  14. Willshaw G. A., Smith H. R., Scotland S. M., Field A.M., Rowe B. Heterogeneity of Escherichia coli phages encoding Vero cytotoxins: comparison of cloned sequences determining VT1 and VT2 and development of specific gene probes. J Gen Microbiol 1987; 133:1309–1317
    [Google Scholar]
  15. Thomas A., Smith H. R., Willshaw G. A., Rowe B. Non-radio-actively labelled polynucleotide and oligonucleotide DNA probes, for selectively detecting Escherichia coli strains producing Vero cytotoxins VT1, VT2 and VT2 variant. Mol Cell Probes 1991; 5:129–135
    [Google Scholar]
  16. Willshaw G. A., Thirlwell J., Jones A. P., Parry S., Salmon R. L., Hickey M. Vero cytotoxin-producing Escherichia coli O157 in beefburgers linked to an outbreak of diarrhoea, haemorrhagic colitis and haemolytic uraemic syndrome in Britain. Lett Appl Microbiol 1994; 19:304–307
    [Google Scholar]
  17. Baudry B., Savarino S. J., Vial P., Kaper J. B., Levine M. M. A sensitive and specific DNA probe to identify enteroaggregative Escherichia coli, a recently discovered diarrheal pathogen. J Infect Dis 1990; 161:1249–1251
    [Google Scholar]
  18. Bilge S. S., Clausen C. R., Lau W., Moseley S. L. Molecular characterization of a fimbrial adhesin, F1845, mediating diffuse adherence of diarrhea-associated Escherichia coli to HEp-2 cells. J Bacteriol 1989; 171:4281–4289
    [Google Scholar]
  19. Scotland S. M., Willshaw G. A., Smith H. R., Gross R. J., Rowe B. Adhesion to cells in culture and plasmid profiles of enteropathogenic Escherichia coli isolated from outbreaks and sporadic cases of infant diarrhoea. J Infect 1989; 19:237–249
    [Google Scholar]
  20. Smith H. R., Scotland S. M., Willshaw G. A., Rowe B., Cravioto A., Eslava C. Isolates of Escherichia coli O44:H18 of diverse origin are enteroaggregative. J Infect Dis 1994; 170:1610–1613
    [Google Scholar]
  21. Scotland S. M., Willshaw G.A., Smith H. R., Rowe B. Properties of strains of Escherichia coli O26:H11 in relation to their enteropathogenic or enterohemorrhagic classification. J Infect Dis 1990; 162:1069–1074
    [Google Scholar]
  22. Donnenberg M. S., Kaper J. B. Enteropathogenic Escherichia coli. Infect Immun 1992; 60:3953–3961
    [Google Scholar]
  23. Girón J. A., Ho A. S. Y., Schoolnik G. K. An inducible bundle-forming pilus of enteropathogenic Escherichia coli. Science 1991; 254:710–713
    [Google Scholar]
  24. Girón J. A., Donnenberg M. S., Martin W. C., Jarvis K. G., Kaper J. B. Distribution of the bundle-forming pilus structural gene (bfpA) among enteropathogenic Escherichia coli. J Infect Dis 1993; 168:1037–1041
    [Google Scholar]
  25. Vial P. A., Robins-Browne R., Lior H. Characterization of enteroadherent-aggregative Escherichia coli, a putative agent of diarrheal disease. J Infect Dis 1988; 158:70–79
    [Google Scholar]
  26. Nataro J. P., Deng Y., Maneval D. R., German A. L., Martin W. C., Levine M. M. Aggregative adherence fimbriae I of enteroag-gregative Escherichia coli mediate adherence to HEp-2 cells and hemagglutination of human erythrocytes. Infect Immun 1992; 60:2297–2304
    [Google Scholar]
  27. Morris K. J., Rao G. G. Conventional screening for enteropathogenic Escherichia coli in the UK. Is it appropriate or necessary?. J Hosp Infect 1992; 21:163–167
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-44-6-438
Loading
/content/journal/jmm/10.1099/00222615-44-6-438
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error