1887

Journal of Medical Microbiology logo

Volume 5, Issue 4

Physiological Properties Of Mutagen-Induced Variants Of Resistant To Polyene Antibiotics Free

Abstract

SUMMARY

Mutants resistant to nystatin have been selected from cultures of a strain of isolated from a patient after it had been treated with N-methyl-N'-nitro-N-nitrosoguanidine. The resistance of the mutants to nystatin, amphotericin B and filipin was respectively 12- to 16-fold, 30- to 40-fold and 3- to 7-fold, more than that of the parent strain. Sensitivity to three non-polyene compounds, clotrimazole, 5-fiuorocytosine and pyrrolnitrin, was not altered. The mutants were identifiable as by standard laboratory tests; two of them seemed to have a decreased ability to form mycelium. There was some loss of acquired resistance after 46 passages . The mutants had more homogeneous patterns of resistance than did a resistant isolate of the same parent strain which had been serially subcultured in the presence of increasing concentrations of nystatin. Mutant cells were found to be of greater eccentricity, and of greater total volume and mass, than those of the parent strain. In shaken culture at 37°C the mutants grew more slowly than the parent; decreased growth rates and prolonged lag phases were also noted for the mutants when grown on solid media at four temperatures. The resistant isolates were agglutinated by similar dilutions of an antiserum to , and the agglutinins were removed by absorption of the antiserum with the parent strain. Possible taxonomic differences arising from loss of mycelium-forming ability of strains are discussed.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1972
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-5-4-425
1972-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/5/4/medmicro-5-4-425.html?itemId=/content/journal/jmm/10.1099/00222615-5-4-425&mimeType=html&fmt=ahah

References

  1. Athar M. A., Winner H. I. 1971; The development of resistance by Candida species to polyene antibiotics in vitro. J. Med. Microbiol 4:505
     [Google Scholar]
  2. Bodenhoff Jessie. 1968; Resistance studies of Candida albicans, with special reference to two patients subjected to prolonged antimycotic treatment. Odont. Tidskr 76:279
     [Google Scholar]
  3. Bodenhoff Jessie. 1969; Development of strains of genus Candida and genus Torulopsis resistant to antimycotics. Acta path, microbiol, scand 75:622
     [Google Scholar]
  4. Borowski E., Zielinski J., Falkowski L., Ziminski T., Golik J., Kolodziejczyk P., Jereczek E., Gdulewicz M., Shenin Y., Kotienko T. 1971; The complete structure of the polyene macrolide antibiotic nystatin Ai. Tetrahedron Lett 685:
     [Google Scholar]
  5. Boudru I. 1969; De la résistance des Candida albicans à la nystatine. J. Pharm. Belg 24:162
     [Google Scholar]
  6. Chu E. H. Y., Malling H. V. 1968; Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro. Proc. Nain. Acad. Sci., U.S.A 61:1306
     [Google Scholar]
  7. Delio V., Hopwood O. A., Friend E. J. 1970; Mutagenesis by N-methyl-N'-nitro- N-nitrosoguanidine (NTG) in Streptomyces coelicolor. Mutat. Res 9:167
     [Google Scholar]
  8. Denny Maire J., Partridge Betty M. 1968; Tetrazolium as an aid in the routine diagnosis of Candida. J. Clin. Path 21:383
     [Google Scholar]
  9. Donovick R., Pansy F. E., Stout H. A., Stauder H., Weinstein M. J., Gold W. 1955 Some in vitro characteristics of nystatin (Mycostatin). In Therapy of fungus diseases: an international symposium edited by Sternberg T. H., Newcomer V. D. Boston: p. 176
     [Google Scholar]
  10. Hejzlar M., Vymola F. 1970; Comparative study of pimaricin and fungicidin in vitro. J. Hyg. Epidem. Microbiol. Immun 14:211
     [Google Scholar]
  11. Hebeka E. K., Solotorovsky M. 1962; Development of strains of Candida albicans resistant to candidin. J. Bad 84:237
     [Google Scholar]
  12. Hebeka E. K., Solotorovosky M. 1965; Development of resistance to polyene antibiotics in Candida albicans. J. Bad 89:1533
     [Google Scholar]
  13. Jund R., Lacroute F. 1970; Genetic and physiological aspects of resistance to 5-fluoropyrimidines in Saccharomyces cerevisiae. J. Bad 102:607
     [Google Scholar]
  14. Lampen J. O. 1969; Amphotericin B and other polyenic antifungal antibiotics. Amer. J. Clin. Path 52:138
     [Google Scholar]
  15. Lampen J. O., Morgan E. R., Slocum Adelaide. 1957; Effect of nystatin on the utilization of substrates by yeast and other fungi. J. Bad 74:297
     [Google Scholar]
  16. Lebeault J. M., Roche B., Duvnjak Z., Azoulay E. 1969; Protoplasts obtained from Candida tropicalis grown on alkanes. J. Bad 100:1218
     [Google Scholar]
  17. Lindegren Gertrude, Hwang Y. L., Oshima Y., Lindegren C. C. 1965; Genetical mutants induced by ethyl methanesulfonate in Saccharomyces. Canad. J. Genet. Cytol 7:491
     [Google Scholar]
  18. Lingens F., Oltmanns O. 1964; Erzeugung und Untersuchung biochemischer Mangelmutanten von Saccharomyces cerevisiae. Z. Naturf 19b:1058
     [Google Scholar]
  19. Littman M. L., Pisano M. A., Lancaster Rosemay. 1958; Induced resistance of Candida species to nystatin and amphotericin B. Antibiotics A 1957-1958 p. 981
     [Google Scholar]
  20. Lones G. W., Peacock C. L. 1959; Alterations in Candida albicans during growth in the presence of amphotericin B. Antibiotics Chemother 9:535
     [Google Scholar]
  21. Mechlinski W., Schaffner C. P., Gants P., Avitabile G. 1970; Structure and absolute configuration of the polyene antibiotic amphotericin B. Tetrahedron Lett3873
     [Google Scholar]
  22. Megnet R. 1965; Screening of auxotrophic mutants of Schizosaccharomyces pombe with 2-deoxyglucose. Mutat. Res 2:328
     [Google Scholar]
  23. Michalska-trenkner Ewa. 1970; Induced resistance of C. albicans strains to sodium salt of N-succinyl perimycin. Chemotherapy 15:19
     [Google Scholar]
  24. Nickerson W. J. 1953; Reduction of inorganic substances by yeasts. I. Extracellular reduction of sulfite by species of Candida. J. Infect. Dis 93:43
     [Google Scholar]
  25. Palumbo S. A., Johnson M. G., Rieck V. T., Witter L. D. 1971; Growth measurements on surface colonies of bacteria. J. Gen. Microbiol 66:137
     [Google Scholar]
  26. Partridge Betty M., Athar M. A., Winner H. I. 1971; Chick embryo inoculation as a pathogenicity test for Candida species. J. Clin. Path 24:645
     [Google Scholar]
  27. Patel P. V., Johnston J. R. 1968; Dominant mutation for nystatin resistance in yeast. Appi. Microbiol 16:164
     [Google Scholar]
  28. Plempel M., Bartmann K., Büchel K. H., Regel E. 1969; Experimental study of a new orally effective broad-spectrum antimycotic agent. Germ. Med. Mon 14:532
     [Google Scholar]
  29. Savage N., Balish E. 1971; Morphology, physiology and virulence of some mutants of Candida albicans. Infect. Immun 3:141
     [Google Scholar]
  30. Sorenson L. J., Mcnall E. G., Sternberg T. H. 1959; The development of strains of Candida albicans and Coccidioides immitis, which are resistant to amphotericin B. Antibiotics A, 1958-1959 p. 920
     [Google Scholar]
  31. Stout H. A., Pagano J. F. 1956; Resistance studies with nystatin. Antibiotics A, 1955-1956 p. 704
     [Google Scholar]
  32. Tsukada Y., Sugimori T. 1971; Induction of auxotrophic mutants from Candida species and their application to L-threonine fermentation. Agrie, biol. Chem 35:1
     [Google Scholar]
  33. Van uden N., Buckley H. 1970; Genus 2. Candida Berkhout. In The yeasts: a taxonomic study, 2nd ed. edited by Lodder J. Amsterdam: p. 893
     [Google Scholar]
  34. Van Uden N., Vidal-leiria M. 1970 Genus 10. Torulopsis Berlese. In The yeasts: a taxonomic study, 2nd ed. edited by Lodder J. Amsterdam: p. 1235
     [Google Scholar]
  35. Van der walt J. P. 1970 Criteria and methods used in classification. In The yeasts: a taxonomic study, 2nd ed. edited by Lodder J. Amsterdam: p. 34
     [Google Scholar]
  36. Wong D. T., Airall Jacqueline M. 1970; The mode of action of antifungal agents: effect of pyrrolnitrin on mitochondrial electron transport. J. Antibiot 23:55
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-5-4-425
Loading
Physiological Properties Of Mutagen-Induced Variants Of Candida Albicans Resistant To Polyene Antibiotics
J. Med. Microbiol. 5, 425 (1972); https://doi.org/10.1099/00222615-5-4-425
/content/journal/jmm/10.1099/00222615-5-4-425
/content/journal/jmm/10.1099/00222615-5-4-425
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error