%0 Journal Article %A Li, Sujuan %A Zhao, Lina %A Zheng, Beiwen %A Shen, Ping %A Ji, Jinru %A Lv, Jifang %A Li, Lanjuan %A Xiao, Yonghong %T Identification and characterization of cfr-positive Staphylococcus aureus isolates from community-onset infectious patients in a county hospital in China %D 2015 %J Journal of Medical Microbiology, %V 64 %N 8 %P 910-915 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.000096 %I Microbiology Society, %X The cfr gene was detected in 14 meticillin-susceptible Staphylococcus aureus isolates recovered from outpatients with community-onset infections in a county hospital in China. The MIC of linezolid was 4 μg ml− 1 in eight isolates and 2 μg ml− 1 in six isolates. All isolates were susceptible to vancomycin and teicoplanin, but had elevated MICs for penicillin (0.5–128 μg ml− 1), chloramphenicol (2–32 μg ml− 1), clindamycin (0.5–128 μg ml− 1) and erythromycin (4–128 μg ml− 1). Nine isolates had mutations on domain V of 23S rRNA and/or the ribosomal L proteins that were not located close to the linezolid-binding pocket. Southern blotting experiments demonstrated that the cfr genes in all 14 isolates resided on plasmids. Sequence analysis of the 5.6 kb cfr-carrying plasmid segment revealed 99 % identity to the corresponding sequences in plasmid pSS-01 from animal staphylococci and plasmid pRM-01 from human staphylococci. Five isolates belonged to sequence type (ST)188 and three to ST965; the two ST types were previously reported in isolates of animal origin in some areas of China. These results indicate that the cfr-carrying plasmids in this study are likely of animal origin. The present study shows that cfr-harbouring S. aureus isolates have emerged in some areas of China and that cfr-carrying isolates may be transmitted between animals and humans. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000096