Identification and characterization of cfr-positive Staphylococcus aureus isolates from community-onset infectious patients in a county hospital in China
Li, Sujuan and Zhao, Lina and Zheng, Beiwen and Shen, Ping and Ji, Jinru and Lv, Jifang and Li, Lanjuan and Xiao, Yonghong,, 64, 910-915 (2015),
doi = https://doi.org/10.1099/jmm.0.000096,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= The cfr gene was detected in 14 meticillin-susceptible Staphylococcus aureus isolates recovered from outpatients with community-onset infections in a county hospital in China. The MIC of linezolid was 4 μg ml− 1 in eight isolates and 2 μg ml− 1 in six isolates. All isolates were susceptible to vancomycin and teicoplanin, but had elevated MICs for penicillin (0.5–128 μg ml− 1), chloramphenicol (2–32 μg ml− 1), clindamycin (0.5–128 μg ml− 1) and erythromycin (4–128 μg ml− 1). Nine isolates had mutations on domain V of 23S rRNA and/or the ribosomal L proteins that were not located close to the linezolid-binding pocket. Southern blotting experiments demonstrated that the cfr genes in all 14 isolates resided on plasmids. Sequence analysis of the 5.6 kb cfr-carrying plasmid segment revealed 99 % identity to the corresponding sequences in plasmid pSS-01 from animal staphylococci and plasmid pRM-01 from human staphylococci. Five isolates belonged to sequence type (ST)188 and three to ST965; the two ST types were previously reported in isolates of animal origin in some areas of China. These results indicate that the cfr-carrying plasmids in this study are likely of animal origin. The present study shows that cfr-harbouring S. aureus isolates have emerged in some areas of China and that cfr-carrying isolates may be transmitted between animals and humans.,
language=,
type=