1887

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Volume 64, Issue 8

Pathological and bacteriological characterization of neonatal porcine diarrhoea of uncertain aetiology Free

Abstract

Neonatal porcine diarrhoea of uncertain aetiology has been reported from a number of countries. This study investigated 50 diarrhoeic and 19 healthy piglets from 10 affected Swedish herds. The piglets were blood-sampled for analysis of serum γ-globulin and necropsied, and the intestines were sampled for histopathology and cultured for , and . isolates ( = 276) were examined by PCR for virulence genes encoding LT, STa, STb, EAST1, VT2e, F4, F5, F6, F18, F41, AIDA-I, intimin, and for the genes and . Selected isolates were analysed for additional virulence genes by a microarray and subjected to O-typing. isolates ( = 152) were examined by PCR for genes encoding major toxins, enterotoxin and beta2-toxin. There was no difference in serum γ-globulin concentration between diarrhoeic and non-diarrhoeic piglets, and pathological lesions in the intestines were generally mild. Porcine enterotoxigenic , a common cause of piglet diarrhoea, was only found in two piglets. Further, the virulence gene profiling did not suggest involvement of other diarrhoeogenic pathotypes of . Growth of did not differ between diarrhoeic and non-diarrhoeic piglets. All isolates were type A, all were negative for enterotoxin, and 151 of 152 isolates were beta2-toxin positive. In pigs ≥ 2 days old, moderate to profuse growth of was more common in the controls. In conclusion, it was not possible to relate , type A and C or to neonatal porcine diarrhoea in any of the investigated herds.

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© 2015 The Authors
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2015-08-01
2024-04-24
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Pathological and bacteriological characterization of neonatal porcine diarrhoea of uncertain aetiology
J. Med. Microbiol. 64, 916 (2015); https://doi.org/10.1099/jmm.0.000108
/content/journal/jmm/10.1099/jmm.0.000108
/content/journal/jmm/10.1099/jmm.0.000108
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