RT Journal Article SR Electronic(1) A1 Sami Kocazeybek, Bekir A1 Caliskan, Reyhan A1 Erdamar Cetin, Sibel A1 Ergin, Sevgi A1 Kuskucu, Mert A1 Kepil, Nuray A1 Oyku Dinc, Harika A1 Ziya Erzin, Yusuf A1 Saribas, Suat A1 Bahar Tokman, Hrisi A1 Kalayci, Fatma A1 Akgul, Ozer A1 Yuksel, Pelin A1 Karakullukcu, Asiye A1 Ziver, Tevhide A1 Sirekbasan, Serhat A1 Caglar, Erkan A1 Bal, KadirYR 2015 T1 Patterns of EPIYA motifs among cagA-positive Helicobacter pylori strains: a case–control study in a Turkish population with Eurasian geographical features JF Journal of Medical Microbiology, VO 64 IS 10 SP 1117 OP 1123 DO https://doi.org/10.1099/jmm.0.000141 PB Microbiology Society, SN 1473-5644, AB Geographical variation in the frequency of various gastroduodenal pathologies was shown to be related to the geographical diversity of H. pylori CagA Glu-Pro-Ile-Tyr-Ala (EPIYA) patterns. We examined the EPIYA patterns of H. pylori and the association of EPIYA patterns with gastric cancer (GC) for the first time, to the best of our knowledge, in Turkey. The patient group (PG) contained 60 patients [38 GC and 22 duodenal ulcer (DU) patients]. The control group (CG) was 110 individuals [94 gastritis patients and 16 persons with a normal gastrointestinal system (NGIS)]. Specific primers were used for the detection of cagA including empty-site-positive and EPIYA-A, -B, -C, -D PCR. Bands of EPIYA-A, -B, -C were confirmed by DNA sequencing. One hundred and forty-two (83.5 %) strains [60 in the PG (38 GC, 22 DU), 82 in the CG (72 gastritis, 10 NGIS)] were positive for the cagA gene. EPIYA-C with multiple repeats was detected in 34 (23.9 %) strains, and 22 (64.7 %) were from GC patients. EPIYA-C with one repeat was detected in 89 (62.7 %) strains, and 54 (60.7 %) were from gastritis patients. EPIYT was detected in 10 strains, and EPIYA-D was not detected. The number of EPIYA-C with multiple repeats was significantly higher for the PG than for the CG (P < 0.0001). In GC patients, the number of EPIYA-C with multiple repeats was significantly higher than one repeat (P < 0.0001). In conclusion, our study showed that multiple EPIYA-C repeats increases the GC risk by 30.6-fold and the DU risk by 8.9-fold versus the CG. This indicates that Western-type H. pylori strains in Turkey have similar EPIYA motifs to those of neighbouring countries and Western populations., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000141