1887

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Volume 64, Issue 11

Prevalence and molecular types of isolates from faecal specimens of patients in a tertiary care centre Free

Abstract

infection (CDI) leads to considerable morbidity and mortality among hospitalized patients. Faecal specimens from 1110 hospitalized patients suspected for CDI were cultured for isolation of and characterization of virulence genes. PCR was carried out for toxigenic genes , , and and PCR-RFLP for and genes. Of 174 (15.7 %) isolates, 121 (69.5 %) were toxigenic, amongst which 68 (56.2 %) also had both and genes. The remaining 53 (43.8 %) of the isolates also had at least one of the toxin genes. Binary toxin genes ( and ) with only one of the two components were present in 16 (9.2 %) of the 174 isolates. The other virulence genes – and – were present in 100 % of the isolates. The most frequent PCR-RFLP type of gene was type I ( = 101), followed by type VII ( = 49) and type III ( = 24). The gene presented with three combinations of patterns. Characterization of virulence genes in isolates is of extreme importance for epidemiological surveillance and control of outbreaks owing to the capacity of this bacterium to adapt to new environmental circumstances, leading to the emergence of new epidemic strains.

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© 2015 The Authors
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2015-11-01
2024-04-25
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Prevalence and molecular types of Clostridium difficile isolates from faecal specimens of patients in a tertiary care centre
J. Med. Microbiol. 64, 1297 (2015); https://doi.org/10.1099/jmm.0.000169
/content/journal/jmm/10.1099/jmm.0.000169
/content/journal/jmm/10.1099/jmm.0.000169
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