Genotypic assessment of drug-resistant tuberculosis in Baghdad and other Iraqi provinces using low-cost and low-density DNA microarrays Al-Rubaye, Dalal Saleh and Henihan, Grace and Al-Abasly, Ameraa K. Abas and Seagar, Amie-Louise and Al-Attraqchi, Azhar Ab. F. and Schulze, Holger and Hashim, Dhafer Salman and Kamil, Jinan Khalid and Laurenson, Ian F. and Bachmann, Till T.,, 65, 114-122 (2016), doi = https://doi.org/10.1099/jmm.0.000203, publicationName = Microbiology Society, issn = 0022-2615, abstract= We report on a molecular investigation carried out to ascertain the prevalence of drug-resistant tuberculosis (TB) and the specific gene mutations responsible for resistance to rifampicin (RIF) and/or isoniazid (INH) in Iraq. In total, 110 clinical isolates from category II TB cases from Baghdad (58 %) and several Iraqi provinces (42 %) were analysed using colorimetric, low-cost and low-density (LCD) microarrays (MYCO-Direct and MYCO-Resist LCD array kits) to identify the point mutations responsible for resistance in Mycobacterium tuberculosis isolates. We found 76 patients (69.1 %) had resistant strains, of which 40 (36 %) were multidrug-resistant (MDR)-TB. Where mono-resistance was identified, it was found to be predominantly to RIF (83 %). The most common mutations were rpoB S531L (50 %), inhA C15T (25 %) and katG S315T (15 %). The most common MDR-TB genotypes were rpoB S531L with inhA C15T (60 %) and rpoB S531L with katG S315T (20 %). Where phenotypic analysis of clinical isolates was also performed, genotypic data were found to show excellent correlation with phenotypic results. Correlation was found between the MYCO-Resist LCD array and GenoType MTBDRplus for detection of resistance to RIF. Our study shows MDR-TB in 36 % of category II TB cases in Baghdad and surrounding Iraqi provinces, which reflects the World Health Organization findings based on phenotypic studies. Diagnosis of TB and MDR-TB using culture-based tests is a significant impediment to global TB control. The LCD arrays investigated herein are easy to use, sensitive and specific molecular tools for TB resistance profiling in resource-limited laboratory settings., language=, type=