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f Escherichia coli strain Nissle 1917 ameliorates experimental colitis by modulating intestinal permeability, the inflammatory response and clinical signs in a faecal transplantation model
- Authors: Éricka L. Souza1 , Samir D. Elian1 , Laís M. Paula1 , Cristiana C. Garcia2 , Angélica T. Vieira1 , Mauro M. Teixeira3 , Rosa M. Arantes4 , Jacques R. Nicoli1 , Flaviano S. Martins1
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1 1Department of Microbiology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil 2 2Fiocruz, Instituto Oswaldo Cruz, Laboratório de vírus Respiratórios e Sarampo, Rio de Janeiro, RJ, Brazil 3 3Department of Biochemistry and Immunology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil 4 4Department of General Pathology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil
- Correspondence Flaviano S. Martins [email protected]
- First Published Online: 01 March 2016, Journal of Medical Microbiology 65: 201-210, doi: 10.1099/jmm.0.000222
- Subject: Pathogenicity and Virulence/Host Response
- Received:
- Accepted:
- Cover date:




Escherichia coli strain Nissle 1917 ameliorates experimental colitis by modulating intestinal permeability, the inflammatory response and clinical signs in a faecal transplantation model, Page 1 of 1
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Inflammatory bowel diseases (IBDs) are a group of inflammatory conditions of the gut that include ulcerative colitis and Crohn's disease. Probiotics are live micro-organisms that may be used as adjuvant therapy for patients with IBD. The aim of this study was to evaluate the effect of prophylactic ingestion of Escherichia coli strain Nissle 1917 (EcN) in a murine model of colitis. For induction of colitis, mice were given a 3.5 % dextran sodium sulfate (DSS) solution for 7 days in drinking water. EcN administration to mice subjected to DSS-induced colitis resulted in significant reduction in clinical and histopathological signs of disease and preservation of intestinal permeability. We observed reduced inflammation, as assessed by reduced levels of neutrophils, eosinophils, chemokines and cytokines. We observed an increase in the number of regulatory T-cells in Peyer's patches. Germ-free mice received faecal content from control or EcN-treated mice and were then subjected to DSS-induced colitis. We observed protection from colitis in animals that were colonized with faecal content from EcN-treated mice. These results suggest that preventative oral administration of EcN or faecal microbiota transplantation with EcN-containing microbiota ameliorates DSS-induced colitis by modifying inflammatory responsiveness to DSS.
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Abbreviations: CD Crohn's disease CV conventional CVZ conventionalized DAI disease activity index DSS dextran sodium sulfate EcN Escherichia coli strain Nissle 1917 EPO eosinophil peroxidase FMT faecal microbiota transplantation GF germ free IBD inflammatory bowel disease MPO myeloperoxidase PE phycoerythrin PP Peyer's patch UC ulcerative colitis
© 2016 The Authors | Published by the Microbiology Society
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